The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

Rudy Agung Nugroho(1), Hetty Manurung(2), Dewi Saraswati(3), Deasy Ladyescha(4), Firman Muhammad Nur(5),


(1) Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda
(3) Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda
(4) Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda
(5) Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda

Abstract

This research aimed to determine the phytochemicals content of Terminalia catappa leaves extract (TCL) and its effects on the survival and blood profiles of ornamental fish (Betta sp) Ninety fish were randomly assigned into six triplicates groups and reared in various concentration of TCL: 0 (control), 125, 250, 375, 500, 625 ppm for 30 days. Temperature, Dissolve oxygen (DO), and pH were monitored during the trial. After 30 days, survival, Red Blood Cells (RBC), White Blood Cells (WBC), haemoglobin (Hb), lymphocyte, and total protein serum (TPS) were analyzed. Based on the phytochemicals test, saponin, triterpenoid, quinon, phenolic, tannin, and flavonoid were detected on the TCL. Temperature and DO were not affected by any concentration of TCL. The lowest pH (5.05) was found in fish medium immersed with 625 ppm of TCL. Adding TCL above 375 ppm resulted in significantly higher survival, RBC, and Hb. The highest WBC was found in fish immersed with 625 ppm whereas the lowest lymphocyte was found in fish immersed with 375 of TCL. However, immersing any various concentration of TCL did not affect on the TPS. In summary, immersing TCL above 375 ppm is beneficial to enhance survival, RBC, WBC, and Hb of Betta sp.

How to Cite

Nugroho, R. A., Manurung, H., Saraswati, D., Ladyescha, D. & Nur, F. M. (2016). The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured. Biosaintifika: Journal of Biology & Biology Education, 8(2), 240-247.

Keywords

Terminalia catappa L.; Betta sp; phytochemical; Survival; haematological profile

Full Text:

PDF

References

Ahilan. B, Nithiyapriyatharshini, A., & Ravaneshwaran, K. (2010). Influence of certain herbal additives on the growth, survival and disease resistance of goldfish, Carassius auratus (Linneaus) Tamilnadu. Journal of Veterinary and Animal Sciences, 6(1), 5-11.

Akah, P., Okpi, O., & Okoli, C. (2008). Evaluation of the anti-inflammatory, analgesic and antimicrobial activities of bark of Afzelia africana. Nigerian Journal of Natural Products and Medicine, 11(1), 48-52.

Akinwande, A., Dada, A., & Moody, F. (2011). Effect of dietary administration of the phytochemical “Genistein”(3, 5, 7, 3, 4 Pentahydroxyflavone) on masculine tilapia, Oreochromis niloticus.

Asgary, S., Naderi, G. H., & Askari, N. M. S. (2005). Protective effect of flavonoids against red blood cell hemolysis by free radicals. Experimental Cardiology, 10(2), 88-90.

Ashraf, M., & Bengtson, D. A. (2007). Effect of tannic acid on feed intake, survival and growth of striped bass (Morone saxatilis) larvae. International Journal of Agriculture and Biology, 9, 751-754.

Ayini, U., B., S. H., & Dewi, T. C. (2014). Efek antibakteri ekstrak daun mimba (Azadirachta indica A. Juss) terhadap bakteri Vibrio algynoliticus secara In vitro. Biosaintifika: Journal of Biology & Biology Education, 6(1), 67-75.

Balachandran, N., & Tissera, K. (2013). A study on the effect of using mangrove leaf extracts as a feed additive in the progress of bacterial infections in marine ornamental fish. Journal of Coastal Life Medicine, 1(3), 217-224.

Ballarin, L., Dall’Oro, M., Bertotto, D., Libertini, A., Francescon, A., & Barbaro, A. (2004). Haematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): a comparison between diploid and triploid specimens. Comparative Biochemical Physiology Part A: Molecular and Integrative Physiology, 138(1), 45-51.

Blaxhall, B. R., & Rao, K. S. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5(6), 771-781.

Chakraborty, S. B., & Hancz, C. (2011). Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in finfish culture. Reviews in Aquaculture, 3(3), 103-119.

Chakraborty, S. B., Horn, P., & Hancz, C. (2014). Application of phytochemicals as growth-promoters and endocrine modulators in fish culture. Reviews in Aquaculture, 6(1), 1-19.

Chakraborty, S. B., Molnár, T., & Hancz, C. (2012). Effects of methyltestosterone, tamoxifen, genistein and Basella alba extract on masculinization of guppy (Poecilia reticulata). Journal of Applied Pharmaceutical Science, 2(12), 48-52.

Chandel, A. K., Lakshmi Narasu, M., Chandrasekhar, G., Manikyam, A., & Venkateswar Rao, L. (2009). Use of Saccharum spontaneum (wild sugarcane) as biomaterial for cell immobilization and modulated ethanol production by thermotolerant Saccharomyces cerevisiae VS3. Bioresource Technology, 100(8), 2404-2410.

Chansue, N., & Assawawongkasem, N. (2008). The in vitro antibacterial activity and ornamental fish toxicity of the water extract of Indian Almond leaves (Terminalia catappa Linn.). KKU Veterinary Journal, 18(1), 36-45.

Chen, P. S., & Li, J. H. (2006). Chemopreventive effect of punicalagin, a novel tannin component isolated from Terminalia catappa, on H-ras-transformed NIH3T3 cells. Toxicology letters, 163(1), 44-53.

Chitmanat, C., Tongdonmuan, K., Khanom, P., Pachontis, P., & Nunsong, W. (2003). Antiparasitic, antibacterial, and antifungal activities derived from a Terminalia catappa solution against some tilapia (Oreochromis niloticus) pathogens. In: III WOCMAP Congress on Medicinal and Aromatic Plants-Volume 4: Targeted Screening of Medicinal and Aromatic Plants, Economics 678. pp. 179-182.

Citarasu, T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquaculture International, 18(3), 403-414.

Clotfelter, E. D., & Rodriguez, A. C. (2006). Behavioral changes in fish exposed to phytoestrogens. Environmental Pollution, 144(3), 833-839.

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical microbiology reviews, 12(4), 564-582.

Ekman, A., Wallberg, O., Joelsson, E., & Börjesson, P. (2013). Possibilities for sustainable biorefineries based on agricultural residues – A case study of potential straw-based ethanol production in Sweden. Applied Energy, 102, 299-308.

Francis, G., Kerem, Z., Makkar, H. P., & Becker, K. (2002). The biological action of saponins in animal systems: a review. British Journal of Nutrition, 88(6), 587-605.

Helal, R., & Melzig, M. F. (2011). In vitro effects of selected saponins on the production and release of lysozyme activity of human monocytic and epithelial cell lines. Scientia Pharmaceutica, 79(2), 337-349.

Hyttel, P., Sinowatz, F., & Vejlsted, M. (2010). Essentials of domestic animal embryology. UK: Elsevier Health Sciences.

Hyttel, P., Sinowatz, F., Vejlsted, M., & Betteridge, K. (2009). Essentials of domestic animal embryology. UK: Elsevier Health Sciences.

Inama, L., Diré, S., Carturan, G., & Cavazza, A. (1993). Entrapment of viable microorganisms by SiO2 sol-gel layers on glass surfaces: Trapping, catalytic performance and immobilization durability of Saccharomyces cerevisiae. Journal of Biotechnology, 30(2), 197-210.

Ji, S. C., Takaoka, O., Jeong, G.-S., Lee, S.-W., Ishimaru, K., Seoka, M., & Takii, K. (2007). Dietary medicinal herbs improve growth and some non-specific immunity of red sea bream Pagrus major. Fisheries Science, 73(1), 63-69.

Kaleeswaran, B., Ilavenil, S., & Ravikumar, S. (2011). Growth response, feed conversion ratio and antiprotease activity of Cynodon dactylon (L.) mixed diet in Catla catla (Ham.). Journal of Animal and Veterinary Advances, 10(4), 511-517.

Karimi, S. H., Kochinian, P., & Salati, A. P. (2013). Short Paper: The effect of sexuality on some haematological parameters of the yellowfin seabream, Acanthopagrus latus in Persian Gulf. Iranian Journal of Veterinary Research, 14(1), 65-68.

Kiparissis, Y. (2001). Effects of flavonoids and other phytochemicals on fish Cypia monooxygenases, embryonic and reproductive development, Trent University, Canada.

Kitagawa, S., Fujisawa, H., & Sakurai, H. (1992). Scavenging effects of dihydric and polyhydric phenols on superoxide anion radicals, studied by electron spin resonance spectrometry. Chemical and Pharmaceutical Bulletin, 40(2), 304-307.

Kori-Siakpere, O., Ake, J. E. G., & Idoge, E. (2005). Haematological characteristics of the African snakehead, Parachanna obscura. African Journal of Biotechnology, 4(6), 527-530.

Lestari, R. D., & Susanti, R. (2015). Effectivity of Pedada Fruit (Sonneratia caseolaris) Extract to The Level of Sgot and Sgpt in Rat Treated by Paracetamol Induction. Biosaintifika: Journal of Biology & Biology Education, 7(1), 29-36.

Lyu, S. Y., & Park, W. B. (2005). Production of cytokine and NO by RAW 264.7 macrophages and PBMC in vitro incubation with flavonoids. Archives of Pharmacal Research, 28(5), 573-581.

Madhuri, S., Mandloi, A. K., Govind, P., & Sahni, Y. P. (2013). Antimicrobial activity of some medicinal plants againts fish pathogen. International Research Journal of Pharmacy, 3, 28-30.

Meena, K., & Raja, T. K. (2006). Immobilization of Saccharomyces cerevisiae cells by gel entrapment using various metal alginates. World Journal of Microbiology and Biotechnolog, 22(6), 651-653.

Metin, K., Koca, Y. B., Kiral, F. K., Koca, S., & Türkozan, O. (2008). Blood cell morphology and plasma biochemistry of captive Mauremys caspica (Gmelin, 1774) and Mauremys rivulata (Valenciennes, 1833). Acta Veterinaria Brno, 77(2), 163-174.

Miksen, C., & Media, D. (2016). Aquariums: How does tannic acid affect aquariums? http://pets.thenest.com/tannic-acid-affect-aquariums-12501.html Accessed 02 June 2016 2016.

Nagesh, T. S., Jayabalan, N., Mohan, C. V., Annappaswamy, T. S., & Anil, T. M. (1999). Survival and histological alterations in juvenile tiger shrimps exposed to saponin. Aquaculture International, 7(3), 159-167.

Nair, M. P. N., Kandaswami, C., Mahajan, S., Chadha, K. C., Chawda, R., Nair, H., Kumar, N., Nair, R. E., & Schwartz, S. A. (2002). The flavonoid, quercetin, differentially regulates Th-1 (IFNγ) and Th-2 (IL4) cytokine gene expression by normal peripheral blood mononuclear cells. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1593(1), 29-36.

Oda, K., Matsuda, H., Murakami, T., Katayama, S., Ohgitani, T., & Yoshikawa, M. (2003). Relationship between adjuvant activity and amphipathic structure of soyasaponins. Vaccine, 21(17), 2145-2151.

Pandey, G. (2013). Some Medicinal Plants to Treat Fish Ectoparasitic Infections. International Journal of Pharmaceutical and Research Sciences, 2(2), 532-538.

Rajput, Z. Q., Hu, S. H., Xiao, C. W., & Arijo, A. G. (2007). Adjuvant effects of saponins on animal immune responses. Journal of Zhejiang University. Science. B, 8(3), 153-161.

Rattanachaikunsopon, P., & Phumkhachorn, P. (2007). Bacteriostatic effect of flavonoids isolated from leaves of Psidium guajava on fish pathogens. Fitoterapia, 78(6), 434-436.

Roy, P. K., & Munshi, J. P. (1989). Effect of saponin extracts on oxygen uptake and hematology of an-breathing climbing perch, Anabas testudineus (Bloch). Journal of Freshwater Biologi, 1(2), 167-172.

Schütt, D. A., Lehmann, J., Goerlich, R., & Hamers, R. (1997). Haematology of swordtail, Xiphophorus helleri. I: blood parameters and light microscopy of blood cells. Journal of Applied Ichthyology, 13(2), 83-89.

Shadu, S. K., Firoj, A., Takashi, O., & Masami, I. (2006). Flavonoids from Sonneratia caseolaris. Journal of Natural Medicines, 60(3), 264-265.

Shatoor, A. S. (2011). Acute and sub-acute toxicity of Crataegus aronia syn. azarolus (L.) whole plant aqueous extract in wistar rats. American Journal of Pharmacology and Toxicology, 6(2), 37-45.

Sivasankar, P., Anix Vivek Santhiya, A., & Kanaga, V. (2015). A review on plants and herbal extracts against viral diseases in aquaculture. Journal of Medicinal Plants Studies, 3(2), 75-79.

Stadlander, T. (2012). Saponin fractions from fenugreek (Trigonella foenum-graecum L.) as dietary supplements for Nile tilapia (Oreochromis niloticus L.) and common carp (Cyprinus carpio L.), Universität Hohenheim, Bremen.

Su, B. K., & Chen, J. C. (2008). Effect of saponin immersion on enhancement of the immune response of white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish & Shellfish Immunology, 24(1), 74-81.

Suely, A., Zabed, H., Ahmed, A. B. A., Mohamad, J., Nasiruddin, M., Sahu, J. N., & Ganesan, P. (2016). Toxicological and hematological effect of Terminalia arjuna bark extract on a freshwater catfish, Heteropneustes fossilis. Fish physiology and biochemistry, 42(2), 431-444.

Syahidah, A., Saad, C. R., Daud, H. M., & Abdelhadi, Y. M. (2015). Status and potential of herbal applications in aquaculture: A review. Iranian Journal of Fisheries Sciences, 14(1), 27-44.

Tanaka, T., Nonaka, G.-I., & Nishioka, I. (1986). Tannins and related compounds. XLII. : Isolation and characterization of four new hydrolyzable tannins, terflavins A and B, tergallagin and tercatain from the leaves of Terminalia catappa L. Chemical & Pharmaceutical Bulletin, 34(3), 1039-1049.

Uboh, F. E., Okon, I. E., & Ekong, M. B. (2010). Effect of aqueous extract of Psidium guajava leaves on liver enzymes, histological integrity and hematological indices in rats. Gastroenterology Research, 3(1), 32-38.

Van–Sumere, C. F., Albrecht, J., Dedonder, A., Depooter, H., & Pe, I. (1975). Plant proteins and phenolics. In: Annual Proceedings of phytochemistry Society,. pp. 211–264.

Vargeshe, J. K., Belzik, N., Nisha, A. R., Resmi, S., & Silvipriya, K. S. (2010). Pharmacognostical and phytochemical studies of a mangrove (Sonneratia caseolaris) from Kochi of Kerala State in India. Journal of Pharmacy research, 3(11), 2625-2627.

Viswaranjan, S., Beena, S., & Palavesam, A. (1988). Effect of tannic acid on the protein, carbohydrate and lipid levels in the tissues of the fish Oreochromis mossambicus. Environmental Ecology, 6(2), 289-292.

Vučurović, V. M., & Razmovski, R. N. (2012). Sugar beet pulp as support for Saccharomyces cerivisiae immobilization in bioethanol production. Industrial Crops and Products, 39, 128-134.

Xiong, S. L., Hou, D. B., Huang, N., & Li, A. (2012). Preparation and biological activity of saponin from Ophiopogon japonicus. African Journal of Pharmacy and Pharmacology, 6(26), 1964-1970.

Zhao, G., Jaiteh, A. A., Wang, W., & Stevens Jr, S. E. (1999). Effects of selected water quality variables on the persistence of tannic acid and related compounds under simulated aquaculture conditions. North American journal of aquaculture, 61(4), 304-309.

Zhou, Z. D., Li, G. Y., & Li, Y. J. (2010). Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate–chitosan beads. International Journal of Biological Macromolecules, 47(1), 21-26.

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.