Grouping of Mangifera indica L. Cultivars of Subang West Java by Leaves Morphology and Anatomy Characteristics

Tri Cahyanto(1), Ahmad Sopian(2), Muhammad Efendi(3), Ida Kinasih(4),


(1) Departement of Biology, Faculty of Science and Technology, Islamic State University Sunan Gunung Djati Bandung, Indonesia
(2) Departement of Biology, Faculty of Science and Technology, Islamic State University Sunan Gunung Djati Bandung, Indonesia
(3) Cibodas Botanic Gardens, Indonesian Institute of Sciences, Indonesia
(4) Departement of Biology, Faculty of Science and Technology, Islamic State University Sunan Gunung Djati Bandung, Indonesia

Abstract

The wide distribution and high species diversity produce large variation in morphology, anatomy and genetics of local mangoes. Until now, most mango classification system is based on the generative character while the vegetative character is neglected. Inventory of mango cultivars in Subang rarely studied, therefore the purpose of this study is to development foundation of identification methods basedthe anatomy and morphology of leavesof mango cultivars of  Subang. The data could futher develop as baseline information about mango cultivar diversity in Indonesia. During study, 21 mango cultivars found in Subang, West Java. Leave samples were collected from the field and soaked in 70% alcohol. Leaf anatomy preparats made by whole-mount method and paraffin. The shape and type, location, size, density and index of stomata were observed. The results showed high similarity of all sampels.There are three variations of vascular tissues on petioles, letter U shape (planoconvex),U lobed (highlyconvex) and round (circular) with the type of drus crystal form, small cubes, and prismatic. All cultivars have actinositic stomata and hypostomatatic leaves. It could be concluded that density, size and stomata index are less powerful to be apply as characters to identified cultivars of mangoes   however the presence of oxalate crystals can be use to the grouping mango cultivars in Subang. 

Keywords

anatomy; morphology; mango; taxonomy; Subang

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References

Aasamaa, K., Sõber, A., & Rahi, M. (2001). Leaf anatomical characteristics associated with shoot hydraulic conductance, stomatal conductance and stomatal sensitivity to changes of leaf water status in temperate deciduous trees. Australian Journal of Plant Physiology, 28(8), 765-774.

Aguoru, C. U., Ajah, P., & Olasan, J. O. (2016). Taxonomic investigation of four varieties of Mangifera using micro-anatomical features. International Research Journal of Natural Sciences, 4(1), 20-27.

Al Afas, N., Marron, N., & Ceulemans, R. (2006). Clonal variation in stomatal characteristics related to biomass production of 12 poplar (Populus) clones in a short rotation coppice culture. Environmental and Experimental Botany, 58(1), 279-286.

Cahyanto, T., Sopian, A., & Efendi. M. (2016). Pengelompokkan sepuluh kultivar mangga asal pamanukan berdasarkan karakter anatomi tangkai dan helaian daun. Prosiding Seminar Nasional Pendidikan Biologi dan Biologi. Jurusan Pendidikan Biologi Fakultas MIPA, Universitas Negeri Yogyakarta, 26 November 2016, 67-74.

Camargo, M. A. B., & Marenco, R. A. (2011). Density, size and distribution of stomata in 35 rainforest tree species in Central Amazonia. Acta Amazonica, 41(2), 205-212.

Damour, G., Simonneau, T., Cochard, H., & Urban, L. (2010). An overview of models of stomatal conductance at the leaf leve. Plant, cell and Environment, 33(9), 1419-1438.

Evert, R. F. (2006). Esau’s Plant Anatomy: Meristems, cells, and tissue of the plant body: their structure, functionand development. New York: John Willey & Sons, INC.

Fahn, A. (1995). Anatomi tumbuhan.Yogyakarta: UGM Press.

Fatihah, H. N. N., Nashriyah, M., Zaimah, A. R. N., Khairil, M. & Ali, A. M. (2014). Leaf morphology and anatomy of 7 varieties of Ficus deltoidea (Moraceae). Turk J Bot, 38(4), 677-685.

Fitmawati, Hartana, A., & Purwoko, B. S. (2009). Taksonomi mangga budidaya Indonesia dalam praktik. Jurnal Agronomi Indonesia, 37(2), 130-137.

Fitmawati, Hartana, A., & Purwoko. B. S. (2010). Diversity of Indonesian mango (mangifera indica) cultivars based on morphological and RAPD markers. SABRAO: Journal of Breeding and Genetics, 42(2), 84-95.

Fitmawati, Suwita, A., Sofiyanti, N., & Herman. (2013). Eksplorasi dan karakterisasi keanekaragaman plasma nutfah mangga (Mangifera) di Sumatera Tengah. Prosiding Semirata FMIPA Universitas Lampung, 307-312.

Franceschi, V. R., & Horner, H. T. (1980). Calcium oxalate crystals in plants. The Botanical Review, 46(4), 361-427.

Ganocpichayagrai, A., Rungsihirunrat, K., Palanuvej, C., & Ruangrungsi, N. (2016). Characterization of Mangifera indica cultivars in Thailand based on macroscopic, microscopic and genetic characters. Journal of Advanced Pharmaceutical Technology & Research, 7(4), 127-133.

Haworth, M., Heath, J., & McElwain, J. C. (2010). Differences in the response sensitivity of stomatal index to atmospheric CO2 among four genera of Cupressaceae conifers. Annals of Botany, 105(3), 411-418.

Hepworth, C., Adams, T. D., Hunt, L., Cameron, D. D., & Gray, J. E. (2015). Manipulating stomatal density enhances drought tolerance without deleterious effect on nutrient uptake. New Phytologist, 208(2), 336-341.

Hetherington, A. M., & Woodward, F. I. (2003). The role of stomata in sensing and driving environmental change. Nature, 424(6951), 901-908.

van Hoof, T. B., Kurschner, W. M., Wagner F., & Visscher, H. (2006). Stomatal index response of Quercus robur and Quercus petraea to the anthropogenic atmospheric CO2 increase. Plant Ecology, 183(2), 237-243.

IPGRI. (2006). Descriptors for Mango (Mangifera indica). Retrieved from http://www.bioversityinternational.org/e-library/publications/detail/descriptors-for-mango-emmangifera-indicaem-l/

Irawan, Y. R., Fitmawati, F., & Herman, H. (2013). Pengetahuan tumbuhan obat dukun sakai desa Sebangar Duri Tiga Belas dan Desa Kesumbo Ampai Duri Kabupaten Bengkalis. Biosaintifika: Journal of Biology & Biology Education, 5(1), 30-35.

Kalyan, C., Tuhinsri, S., Arvind, K.C., & Samir, K.S. (2012). Leaf characters and measure-ments of mango cultivars in Gangetic plains of West Bengal. In: International Seminar on Multidisciplinary Approaches in Angiosperm Systematics, 12 September 2012, Kalyani, WB, 762–768.

Körner, C., Bannister, P., & Mark, A. F. (1986). Altitudinal variation in stomatal conductance, nitrogen content and leaf anatomy in different plant life forms in New Zealand. Oecologia, 69(4), 577-588.

Kouwenberg, L. L. R., Kürschner, W. M., & McElwain, J. C. (2007). Stomatal frequency change over altitudinal gradients: prospects for paleoaltrimetry. Reviews in Mineralogy and Geochemistry, 66(1), 215-241.

Luvaha, E., Netondo, G. W., & Ouma, G. (2008). Effect of water deficit on the physiological and morphological characteristics of mango (Mangifera indica) rootstock seedlings. American Journal of Plant Physiology, 3(1), 1-15.

McElwain, J. C. (2004). Climate-independent paleoaltimetry using stomatal density in fossil leaves as a proxy for CO2 partial pressure. Geology, 32(12), 1017-1020.

Mhamed, Z. M.A., & Ahmed, T. M. H. (2015). Diversity of Mango (Mangifera indica L.) cultivars in Shendi Area: Morphological Leaf Characterization. International Journal of Research in Agricultural Sciences, 2(4), 167-172.

Murray, R. R., Emblow, M. S. M, Hetherington, A. M., & Foster, G. D. (2016). Plant virus infections control stomatal development. Scientific Reports, 6, 34507.

Nakata, P. A., & McConn, M. M. (2000). Isolation of Medicago truncatula Mutants Defective in Calcium Oxalate Crystal Formation. Plant Physiology, 124(3), 1097-1104.

Nilasari, A. N., Heddy, J. S., & Wardiyati, T. (2013). Identifikasi keragaman morfologi daun mangga (Mangifera indica L.) pada tanaman hasil persilangan antara varietas arumanis 143 dengan podang urang umur 2 tahun. Jurnal Produksi Tanaman, 1(1), 61–69.

Ojima, M., Kawashima, R., & Mikoshiba, K. (1969). Studies on the seed production of soybean. VII. The ability of photosynthesis in F1 and F2 generation. Proceedings of the Crop Science Society of Japan, 38, 693–699.

Pakpahan, F. W., Fitmawati & Sofianty, N. (2012). Analisis hubungan kekerabatan mangga (Mangifera sp.) di KabupatenF Kampar Berdasarkan Karakter Morfologi. Skripsi. Riau: Jurusan Matematika dan Ilmu Pengetahuan Alam.Universitas Riau

Perwati, L. K. (2009). Analisis derajat ploidi dan pengaruhnya terhadap variasi ukuran stomata dan spora pada Adiantum raddianum. Bioma, 11(2), 39-44.

Prabhakar, M. (2004). Structure, delimitation, nomenclature and classification of stomata. Acta Botanica Sinica, 46(2), 242–252.

Pugnaire, F. I. & Pardos, J. (1999). Constrains by water stress on plant growth. In Passarakli, M. (ed.) Hand Book of Plant and Crop Stress. New York: J Wiley.

Qosim, W. A., Purwanto, R., Wattimena, G. A. & Witjaksono. (2007). Perubahan anatomi daun pada regeneran manggis akibati radiasi sinar gamma in vitro. Zuriat, 18(1), 20-30.

Qosim, W. A., Purwanto, R., Wattimena, G. A. & Witjaksono. (2011). Alteration of leaf anatomy of mangosteen (Garcinia mangostana L.) regenerants in vitro by gamma irradiation. Plant Mutation Reports, 2(3), 4-11.

Rahayu, Y., Firmawati, & Herman. (2013). Analisis Kenakeragaman Sagu (Metroxylon sagu Rottb.) pada Tiga Tipe Habitat di Pulau Padang Kepulauan Meranti. Biosaintifika: Journal of Biology & Biology Education, 5(1), 17–24.

Rohlf, F. J. (1998). NTSys-PC. Numerical taxonomy and multivariate analysis system.Version 2.02. Exerter Software. New York. h 105-110.

Royer, D. L. (2001). Stomatal density and stomatal index as indicators of paleoatmospheric CO2 concentration. Review of Palaeobotany and Palynology, 114(1), 1-28.

Rudall, P. J. (2007). Anatomy of flowering plants.An Introducation to Structure and Development. New York: Cambridge University Press.

Rugayah & Tihurua, E. F. (2011). Leaf anatomy of Artabotrys suaveolens Blume var. suaveolens and Artabotrys suaveolens Blume var. parviflorusMiquel (Annonaceae). Floribunda, 4(2), 54-56.

Rymbai, H., Laxman, R. H., Dinesh, M. R., Sunoj, V. S. J., Ravishankar, K. V., & Jha, A. K. (2014). Diversity in leaf morphology and physiological characteristics among mango (Mangifera indica) cultivars popular in different agro-climaticregions of India. Scientia Horticulturae, 176, 189-193.

Salisbury, E. J. (1927). On the causes and ecological significance of stomatal frequency with special reference to the woodland flora. Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 216, 1-65.

Sass, J. E. (1951). Botanical Michrotechnioque. 2nd edition. USA: The Lowa State College Press. Lowa.

Schweingruber, F. H., Borner, A. & Schulze, E. D. (2000). Atlas of Steem Anatomy in Herbs, Shrubs and Trees Volume 1. London: Springer Heidelberg Dordrecht.

Sharma, B. G., Albert, S., & Dhaduk, H. K. (2012). Petiolar anatomy as an aid to the identification of Mangifera indica L . Notulae Scientia Biologicae 4(1), 44–47.

Sreelakshmi, V. V., Sruthy, E. P. M., & Shereena, J. (2014) Relationship between the leaf area and taxonomic importance of foliar stomata. IMPACT: International Journal of Research in Applied, Natural, and Social Sciences, 2(7), 53-60.

Sumardi, I., & Wulandari, M. (2010). Anatomy and morphology character of five Indonesian banana cultivars (Musa spp.) of different ploidy level. Biodiversitas, 11(4), 167-175.

Sumiasri, N., Jitno, R. & Doddy, P. (2006). The species and cultivars of mango in Madiun and its surroundings; the development and its problems. Biodiversitas, Journal of Biological Diversity, 7(1), 39-43.

Takayoshi, K., Mitsutoshi, K., Yutaka, A., Shigeta, M., & Thomas, T. L. (2001). Leaf mor-phology and photosynthetic adjustments among deciduous broad-leaved treeswithin the vertical canopy profile. Tree Physiology, 21(12), 951–958.

Tambaru, E., Paembonan, S. A., Sanusi, D., & Umar, A. (2011). Karakter morfologi dan tipe stomata daun beberapa jenis pohon penghijauan hutan kota di kota Makassar. Dissertation. Makassar: Pascasarjana Universitas Hasanuddin Makassar.

Viecelli, J. C., Siqueira, D. L., Bispo, W. M. D. S., & Lemos, L. M. C. (2016) Characterization of leaves and fruits of mango (Mangifera indica L.) CV. Imbu. Revista Brasileira de Fruticultura, 38(3), e-193.

Wahdan, M. T., Abdelsalam, A. Z., El-Naggar, A. A. & Hussein, M. A. (2011). Preliminary horticultural studies to describe and identify of two new Egyptian mango strain using DNA fingerprint. Journal of America Science, 7(2), 641-650.

Widjaja, E. A., Rahayuningsih, Y., Rahajoe, J. S., Ubaidah, R., Maryanto, I., Waluyo, E.B., Waluyo & Semiadi, G. (2014). Kekiniankeanekaragamanhayati Indonesia 2014. Jakarta: LIPI Press.

Woodward, F. I. (1986). Ecophysiological studies on the shrub Vaccinium myrtillus L. taken from a wide altitudinal range. Oecologia, 70(4), 580-586.

Woodward, F. I. (1987). Stomatal numbers are sensitive to increases in CO2 from preindustrial levels. Nature, 327(6123), 617-618.

Woodward, F. I., & Kelly, C. K. (1995). The influence of CO2 concentration on stomatal density. New Phytologist, 131(3), 311-327.

Xu, Z., & Zhou, G. (2008). Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. Journal of Experimental Botany, 59(12), 3317–3325.

Yuniati, E., Tadulako, U., Bumi, K., & Tondo, T. (2010). Etnobotani keanekaragaman jenis tumbuhan obat tradisional dari Hutan di Desa Pakuli Kecamatan Gumbasa Kabupaten Donggala, Sulawesi Tengah. Biocelebes, 4(1), 69-75.

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