KANDUNGAN L-DOPA DALAM VARIASI PERENDAMAN DAN PEREBUSAN DALAM PROSES PEMBUATAN TEMPE BENGUK

Sri Winarni(1), Yudhy Dharmawan(2),


(1) Fakultas Kesehatan Masyarakat, Universitas Diponegoro Semarang
(2) Fakultas Kesehatan Masyarakat, Universitas Diponegoro Semarang

Abstract

Abstrak

L-Dopa biji koro benguk sebesar 14,7%, berbeda dengan tempe benguk. Tujuan penelitian adalah menguji L-Dopa dalam 4 pengolahan tempe benguk yang berbeda. Penelitian tahun 2015 ini menggunakan teknik pengumpulan data dengan mengamati proses pengolahan tempe benguk dan menguji L-Dopa dalam fraksi tempe benguk dengan HPLC. Populasi penelitian adalah seluruh pemilik industri tempe benguk. Sampel penelitian diambil dengan metode purposif sampling, menentukan 4 industri tempe benguk yang sering digunakan. Proses pertama dengan satu rebusan 1-1,5 jam dan satu rendaman (2 hari) mengandung L-Dopa 8425,00 ppm. Proses kedua yaitu rebusan dua kali 1-1,5 jam dan ulangan rendaman tiga kali (setiap rendaman 1 hari 1 malam) mengandung L-Dopa 389,42 ppm. Proses ketiga yaitu ulangan rebusan dua kali (1,5-2 jam) dan rendaman satu kali mengandung L-Dopa 2163,37 ppm. Proses keempat rebusan satu kali (< 1 jam) dan rendaman satu kali (dua hari) mengandung L-Dopa tertinggi yaitu 9781,55 ppm.

Kata kunci : Tempe benguk; Perendaman Perebusan; L-Dopa

 

Abtract

L-Dopa of koro benguk seed is around 14,7%, different with benguk tempeh. The objective of this study is to test L-Dopa in 4 different ways. Data were collected in 2015 by observing benguk tempeh processing and testing L-Dopa in benguk tempeh  (HPLC). The population of this study are all owners of benguk tempeh industries. The sample was taken by using purposive sampling, determining 4 benguk tempeh industries that mostly used. The first process with one boiling 1-1,5 hours and  one immersion (2 days) contain L-Dopa 8425,00 ppm. The second process with two boiling 1-1,5 hours and repetition immersion three times (each immersion 1 day 1 night) contains L-Dopa 389,42 ppm. The third process is twice re-boiling once (< 1 hour)  and immersion once again (2 days) contain the high L-Dopa that is 9781,55 ppm.

Keyword : Benguk tempeh; Soaking-boiling; L-Dopa

Keywords

L-DOPA; modifying process; tempe benguk; spermatozoa quality; food product

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