Abstract

Natural immunomodulators are obtained from herbal plants with natural antioxidant content.
The content of polyphenol compounds as natural antioxidants in katuk leaves and cocoa beans
is utilized in the form of instant powder preparations.
This study aimed to determine the levels of polyphenols and the antioxidant activity of instant
powder combination of katuk leaves and cocoa beans.
Material dan Methods : this study used a quantitative descriptive method with test parameters
including organoleptic, moisture content, ash content, polyphenol qualitative, polyphenol
content and antioxidant activity.
The results of the instant powder moisture content test were 1.71 ± 0.04%; 1.68 ± 0.03%; and
1.59 ± 0.03%. The results of the instant powder ash content test were 1.16 ± 0.03%; 1.18 ±
0.04%; and 1.11 ± 0.03%. The positive instant powder qualitative test results contained
polyphenols as indicated by the formation of blackish-brown color. Polyphenol levels in instant
powder were 43.83 ± 1.76 mg GAE/g; 34.40 ± 0.71 mg GAE/g; and 29.50 ± 0.76 mg GAE/g.
The instant powder is classified as a medium antioxidant category with an IC50 value of 119.25
ppm respectively; 129.25 ppm; and 146,40 ppm.
The Conclusion Formula 1 is a formula that has a large polyphenol content and strong
antioxidant activity.