Alkaloid Compounds from Mahogany Seeds: Isolation and Antimicrobial Activity

  • Sri Mursiti Universitas Negeri Semarang, Indonesia
  • Supartono Supartono Universitas Negeri Semarang, Indonesia


Alkaloid is one of the secondary metabolites compound in mahogany seeds. This study aims to determine the antimicrobial activity of alkaloid compounds from mahogany seeds against Escherichia coli (E.coli) and Bacillus cereus (B.cereus). Isolation of alkaloid compounds done step by step. First, the maceration using n-hexane, then with methanol. The methanol extract was dissolved in Oxalic acid and diethyl ether, then separated. The acid solution was added 10 % ammonia solution, CHCl3, then separated.  The CHCl3 extract was containing primary, secondary, and tertiary alkaloid. The base phase was containing quartenery alkaloid. The testing of antimicrobial activity of flavonoid compounds using the absorption method. The results showed that the antimicrobial activity of alkaloid compounds from mahogany seeds shows the inhibitory activity and provide no clear zone against bacteria E.coli and 15,05 mm to the bacteria B.cereus. Based on the results of the study, it can be concluded that alkaloid compounds from mahogany seeds have antimicrobial activity against B.cereus.