Gold Nanoparticles of Lactobacillus kimchicus DCY51T Suppressed Lipopolysaccharide-Stimulated Inflammatory Reactions in RAW264.7 Macrophage Cells

Abstract

Excessive inflammation can lead to chronic diseases, necessitating the development of effective anti-inflammatory therapies. This study investigates the anti-inflammatory effects of gold nanoparticles (AuNps) synthesized using Lactobacillus kimchicus DCY51^T, a probiotic strain from Korean kimchi. The objective is to explore the potential of DCY51^T-AuNps in modulating inflammatory responses in RAW264.7 macrophage cells. Methods: AuNps were synthesized through a meticulous procedure involving the probiotic Lactobacillus kimchicus DCY51^T. The isolation and subsequent cultivation of the RAW264.7 macrophage cell line in the DMEM medium formed the basis for the research methodology. Results: Following the establishment of the cellular platform, the cells were treated with DCY51^T-AuNps, revealing a noteworthy reduction in the expression of key inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). A pivotal aspect of the study focused on the inhibitory effects of DCY51^T-AuNps on the lipopolysaccharide (LPS)-induced activation of the NF-κB/mitogen-activated protein kinase (MAPK) pathway within RAW264.7 cells. Conclusion: This inhibition was instrumental in modulating the inflammatory response, showcasing the potential therapeutic relevance of DCY51T-AuNps in combating inflammation. The novelty lies in using a probiotic-derived synthesis method, offering a sustainable and biocompatible approach to nanoparticle production. The benefit of this research not only underscores the anti-inflammatory prowess of DCY51^T-AuNps on RAW264.7 macrophages but also contributes to the broader scientific discourse on the biomedical applications of gold nanoparticles synthesized from probiotic sources, potentially benefiting the development of new anti-inflammatory therapies and advancing nanomedicine.