Potential of Sodium Alginate in Sargassum sp. in Lotion Preparation to Treat Incision Wound in Mice
(1) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University
(2) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University
(3) Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University
Abstract
Sargassum sp. is a known genus of brown seaweed whose dominant component is alginic acid or alginate. Various studies found that alginate having proinflammatory activity, so it may have potential to improve the wound healing process. This study aimed to obtain sodium alginate extract and to know the potency of the sodium alginate extract in incision wound treatment in mice abdomen. Lotion application was performed once a day in a span of five consecutive days. Observation on the wound was carried out to obtain data on blood coagulation, tissue reparation, and fibroblast development rate on the wound using histology. The results showed that the average yield from the Sargassum sp. extraction process was 31.3%. Quality control of the prepared lotion included organoleptic test; light brown coloration, lotion specific odor, semi- solid texture. The acidity of the lotion was measured as 6.61 with 6.731 cp of viscosity. The lotion adhesiveness test showed 0.21 seconds and the lotion was shown to be oil-in-water. Application of the lotion on incision wounds made in the abdomen of rats showed blood coagulation on the first day and onset of wound reparation process on the second day. Wound observation by the third day showed that the inflicted area had undergone a near-complete reparation. The study also showed that on the fifth day of reparation Sargassum sp. extract lotion treatment group showed fibroblast formation, as opposed to the non-extract lotion treatment group. Previous study use alginates as wound dressing material, but in this study alginate used as active ingredient in wound healing treatment. Therefore, this study concludes that sodium alginate in Sargassum sp. seaweed has a potential application in the field of medicine.
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Chattopadhyay, S. & R. T. Raines. (2014). Collagen-based biomaterials for wound healing. Biopolymers 101: 821-833.
Chung, M. Y., C. H. Liu, & Y. N. Chen. (2011). Enhancing the reproductive performance of tiger shrimp, (Penaeus monodon) by incorporating sodium alginate in the broodstock and larval diets. Aquaculture 277(1): 66-72.
Cong, Q., F. Xiao, W. Liao, Q. Dong, & K. Ding. (2014). Structure and biological activities of an alginate from (Sargassum fusiforme) and its sulfated derivative. Int. J. Biol. Macromolecules 69:252-259.
Culling, C. F. A and W. L. Dunn. (1974). Handbook of histophatological and histochemical techniques. 3rd edition. Butterworths & Co Publishes, England, New Zealand, Austria, Canada, South Africa, USA.
Dai, T., G. B. Kharkwal, M. Tanaka, Y. Huang, V. J. B. de Arce, and M. R. Hamblin. (2011). Animal models of external traumatic wound infections. Journal of Virulence 2 (4).
Devi, M. P., M. Sekar, M. Chamundeswari, A. Moorty, G. Krithiga, N. S. Murugan, and T. P. Sastry. (2012). A novel wound dressing material-fibrin-chitosan-sodium alginate composite sheet. Bulletin of Materials Science 35(7).
Draget, Kurt I. & Catherine Taylor. 2011. Chemical, physical and biological properties of alginates and their biomedical implications. Food Hydrocolloids 25. Page: 251-256.
Genetics Home References. (2014). Fibroblast. U.S National Library of Medicine.
Harikrishnan, R., Kim, M.C., Kim, J.S., Han, Y.J., Jang, I.S., Balasundaram, C. & Heo, M.S. (2011). Immunomodulatory effect of sodium alginate enriched diet in kelp grouper (Ephinephelus brneus) against Streptococcus iniae. Fish & Shellfish Immunology 30(2):543-549.
Jones & Bartlett. 2011. First aid for families. American Academy of Pediatrics. ISBN 978-0763755522.
Jung, M. K., J. J. Callaci, K. L. Lauing, J. S. Otis, K. A. Radek, M. K. Jones, E. J. Kovacs. (2011). Alcohol exposures and mechanisms of tissue injury and repair. Alcohol Clin Exp Res 35: 392-399.
Kézia O.A., L. Lins, M. L. Vale, R. A. Ribeiro & L. V. Costa-Lotufo. (2013). Proinflammatory activity of an alginate isolated from Sargassum vulgare. Carbohydrate Polymers 92: 414–420.
Kim, E. Y. & K. D. Moudgil. (2017). Immunomodulation of autoimmune arthritis by pro-inflammatory cytokines. Cytokine 98: 87-96.
Koehler, J., F. P. Brandl & A. M. Goepferich. (2018). Hydrogel wound dressings for bioactive treatment of acute and chronic wounds. European Polymer Journal 100: 1-11.
Liu, Y., Lai, Q. Lai, C. Dong, F. Sun, L. Wang, G. Li, and Z. Shao. (2013). Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis. PLOSOne 8 (11): e80097.
Luna, L.G. (1968). Manual histologic staining methods of pathology. 3rd edition. The Blakison Division Mc Graw-hill Book Company, New York, Toronto, London, Sydney.
Luo, Y. & S. G. Zheng. (2016). Hall of fame among pro-inflammatory cytokines: interleukin-6 gene and its transcriptional regulation mechanisms. Front. Immunol.
Maleki, S., M. Maerk, R. Hrudikova, S. Valla, & H. Ertesvag. (2017). New insight into Pseudomonas flourescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates. New Biotechnology 37: 2-8.
Marchaban & Saifullah, T.N. (2014). Petunjuk praktikum formulasi dan teknologi sediaan cair semi padat. Yogyakarta, Gajah Mada University Press.
Megantara, I. N. A. P, Megayanti K., Wirayanti R., Esa I. B. D., Wijayanti N. P. A. D, Yustiantara P.S. (2017). Formulasi lotion ekstrak buah raspberry (Rubus rosifolius) dengan variasi konsentrasi trietanolamin sebagai emulgator serta uji hedonik terhadap lotion. Jurnal Farmasi Udayana 6 (1). ISSN: 2301-7716.
Olesiuk, A. K., M. Nowacka, M. Wesoly, P. Ciosek. (2013). Evaluation of organoleptic and texture properties of dried apples by hybrid electronic tongue. Sensors and Actuators B 187: 234-240.
Osorio, C., S. Machado, J. Peixoto, S. Bessada, F. B. Pimentel, R. C. Alves, & M. B. P. P. Oliveira. (2020). Pigment content (chlorophylls, fucoxhantin and phycobiliproteins) of different commercial dried algae. Separations 7: 33.
Rachmawati, I. (2014). Perbedaan efek perawatan luka menggunakan gerusan daun petai cina (Leucaena glauca, Benth) dan Povidon Iodine 10% Dalam Mempercepat Penyembuhan Luka Bersih Pada Marmut (Cavia pocellus). Jurnal Wiyata 1 (2).
Sellimi, S., I. Younes, H. B. Ayed, H. Maalej, V. Montero, M. Rinaudo, M. Dahia, T. Mechichi, M. Hajji & M. Nasri. (2015). Structural, physicochemical and antioxidant properties of sodium alginate isolated from tunisian brown seaweed. International Journal of Biological Macromolecules 72. Page: 1358-1367.
Stunova, A. & L. Vistejnova. (2018). Dermal fibroblast- a heterogenous population with regulatory function in wound healing. Cytokine and Growth Factor Reviews 39: 137-150.
Suwiti, N. K. (2010). Deteksi histologik kesembuhan luka pada kulit pasca pemberian daun mengkudu (Morinda citrifolia Linn). Buletin Veteriner Udayana.2 (1): 1-9.
Thomas, A., Harding, K. G., & Moore, K. (2000). Alginates from wound dressings activate human macrophages to secrete tumour necrosis factor-α. biomaterials, 21, 1797-1802.
Wosgrau, A. C. C., T. S. Jeremias, D. F. Leonardi, M. J. Pereima, G. D. Giunta, and A. G. Trentin. (2015). Comparative experimental study of wound healing in mice: Perlnac versus Integra. PloS ONE 10(3): e0120322.
Yudiati, E., A. Isnansetyo, Murwantoko, Ayuningtyas, Triyanto, and C. R. Handayani. (2016). Innate imune-stimulating and immune genes up-regulating actvities of three types of alginate from Sargassum siliquosum in pasific white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology 54: 46-53.
Yudiati, E. and A. Isnansetyo. (2017). Characterizing the three different alginate type of Sargassum siliquosum. Jurnal Ilmu Kelautan 22(1): 7-14.
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