Histopathological of White Rats Aorta Induced by High-Fat Feed After Administered by Neem Leaf Ethanolic Extract

Baiq Miftahul Jannah(1), Sri Isdadiyanto(2), Agung Janika Sitasiwi(3),

DOI: https://doi.org/10.15294/biosaintifika.v12i3.25494


(1) Departement of Biology, Faculty of Science and Mathematics, Universitas Diponegoro
(2) Departement of Biology, Faculty of Science and Mathematics, Universitas Diponegoro
(3) Departement of Biology, Faculty of Science and Mathematics, Universitas Diponegoro

Abstract

Neem (Azadirachta indica A. Juss) is one of the traditional medicines used by local people as antioxidants, anti-hyperlipidemia, anti-inflammatory, and treatment for other diseases such as heart disease. This study aimed to analyze the effect of neem leaf ethanolic extract on aortic wall thickness and aortic lumen diameter of white rats induced by high-fat feed. The male Wistar rats (Rattus norvegicus L.) with 2 months of age were used. This research was an experimental study with a Randomized Complete Block Design consisted of 6 treatments (P0: commercial feed, P1: high-fat feed and duck egg yolk 2.5 ml/200 g BW per oral, P2: P1 + 8 mg/200 g BW simvastatin, P3-P5: P1+ neem leaf extract of 75, 100, and 125 mg/200 g BW respectively) and 4 replications. Aortic preparations were made by the paraffin method and Hematoxylin-Eosin staining. Data were analyzed using ANOVA followed by Duncan Multiple Range Test with 95% confidence level. The result showed that the decrease in wall thickening and lumen narrowing was getting higher along with the increasing doses of ethanol extract of neem leaves. Based on the result of this research, it was found that the high doses of neem leaf ethanolic extract has the same ability with simvastatin to reduce aortic wall thickning and aortic lumen narrowing of white rats. This study is give a new information that the neem leaves can be used as an alternative medicine for cardiovascular disease.

Keywords

Aortic Lumen Diameter; Aortic Wall Thickness; High-Fat Feed; Neem; White rats

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References

Aathira, E. P., & Suganthi, A. (2019). Phytochemical Analysis and Anti-Head Lice Activity of Azadirachta indica (A. Juss) and Aegle marmelos (Corr.) (Leaves). World Journal of Pharmaceutical Research, 8(5), 1370–1380.

Ahmad, S., Maqbool, A., Srivastava, A., & Gogoi, S. (2019). Biological Detail and Therapeutic Effect of Azadirachta Indica (Neem Tree) Products- a Review. Journal of Evidence Based Medicine and Healthcare, 6(22), 1607–1612.

Airaodion, A. I., Olatoyinbo, P. O., Ogbuagu, U., Ogbuagu, E. O., Akinmolayan, J. D., Adekale, O. A., Awosanya, O. O., Agunbiade, A. P., Oloruntoba, A. P., Obajimi, O. O., Adeniji, A. R., & Airaodion, E. O. (2019). Comparative Assessment of Phytochemical Content and Antioxidant Potential of Azadirachta indica and Parquetina nigrescens Leaves. Asian Plant Research Journal, 2(3), 1–14.

Alakhali, K., Hassan, Y., Mohamed, N., & Mordi, M. N. (2013). Pharmacokinetic of Simvastatin Study in Malaysian Subjects. IOSR Journal of Pharmacy (IOSRPHR), 3(1), 46–51.

Ayu, A., Rahmawati, F., & Zukhri, S. (2015). Pengaruh Penggunaan Berulang Minyak Goreng terhadap Peningkatan Kadar Asam Lemak Bebas dengan Metode Alkalimetri. Cerata Journal Of Pharmacy Science, 6(1), 1–7.

Bisht, S., & Sisodia, S. S. (2010). Anti-Hyperglycemic and Antidyslipidemic Potential of Azadirachta indica Leaf Extract in STZ-Induced Diabetes Mellitus. Journal of Pharmaceutical Sciences and Research, 2(10), 622–627.

Brühl, L. (2014). Fatty Acid Alterations in Oils and Fats During Heating and Frying. European Journal of Lipid Science and Technology, 116(6), 707–715.

Christianty, F. M., Holidah, D., Fajrin, F. A., Cinuradha, M., Salsabina, A., & Roni, A. (2020). Profil Lipid dan Gambaran Histopatologi Aorta Tikus Hiperlipidemia dengan Pemberian Ekstrak Kopi Hijau (The Lipid Profile and Aorta Histopathology on Hyperlipidemic Rat by Giving Green Coffee Extract). Jurnal Ilmu Kefarmasian Indonesia, 18(1), 21–27.

Cui, Y., Hou, P., Li, F., Liu, Q., Qin, S., Zhou, G., Xu, X., Si, Y., & Guo, S. (2017). Quercetin Improves Macrophage Reverse Cholesterol Transport in Apolipoprotein E-deficient Mice Fed a High-Fat Diet. Lipids in Health and Disease, 16(1), 3–9.

Esenwa, C. C., & Elkind, M. S. (2016). Inflammatory Risk Factors, Biomarkers and Associated Therapy in Ischaemic Stroke. Nature Reviews Neurology, 12(10), 594–604.

Fatimatuzzahro, N., & Prasetya, R. C. (2018). Efek Seduhan Kopi Robusta terhadap Profil Lipid Darah dan Berat Badan Tikus yang Diinduksi Diet Tinggi Lemak. Jurnal Kedokteran Brawijaya, 30(1), 7–11.

Fisher, E. A., Feig, J. E., Hewing, B., Hazen, S. L., & Smith, J. D. (2012). High-Density Lipoprotein Function, Dysfunction, and Reverse Cholesterol Transport. Arteriosclerosis, Thrombosis, and Vascular Biology, 32(12), 2813–2820.

Gravatt, L. A. H., Flurie, R. W., Lajthia, E., & Dixon, D. L. (2017). Clinical Guidance for Managing Statin and Antimicrobial Drug-Drug Interactions. Current Atherosclerosis Reports, 19(11), 1–8.

Hanung, A., Saktini, F., & Gumay, A. R. (2019). Pengaruh Frekuensi Penggorengan Minyak Jelantah Terhadap Diameter Dan Gambaran Histopatologi Lumen Aorta Tikus Wistar (Rattus Novergicus). Jurnal Kedokteran Diponegoro, 8(1), 26–37.

Hayatunnufus, R., Wati, G. P. E., & Budianto, E. (2019). Preparation, Characterization, and Encapsulation Efficiency Test of Simvastatin Microencapsulation Using Polyblend of Poly(Lactic Acid) and Poly(ɛ-Caprolactone). Materials Science Forum, 947, 21–25.

Ifora, Dharma, S., & Darma, D. M. (2016). Pengaruh Pemberian Kombinasi Jahe Merah, Bawang Putih, Apel, Lemon Dan Madu Terhadap Kadar Kolesterol Total Dan Histopatologis Pembuluh Darah Aorta Jantung Tikus Putih Jantan. Jurnal Farmasi Higea, 8(2), 163–174.

Isdadiyanto, S., Sitasiwi, A. J., & Mardiati, S. M. (2020). The Lipid Profile of Rats (Rattus norvegicus L.) Induced by High Fat Ration after Exposed to Ethanolic Neem (Azadirazchta indica) Leaf Extract. Journal of Physics: Conference Series.

Islam, M., Al-Amin, M., Siddiqi, M. M. A., Akter, S., Haque, M. M., Sultana, N., & Chowdhury, A. S. (2012). Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta indica and Antimicrobial and Cytotoxic Screening of the Crude Extracts. Dhaka University Journal of Science, 60(1), 11–14.

Lee, W., Ku, S. K., & Bae, J. S. (2012). Barrier Protective Effects of Rutin in LPS-induced Inflammation in vitro and in vivo. Food and Chemical Toxicology, 50(9), 3048–3055.

Linna, M. (2014). Oxidized LDL Lipids As a Risk Factor for Atherosclerosis.

Lozano, R., Naghavi, M., Foreman, K., Lim, S., Shibuya, K., Aboyans, V., Abraham, J., Adair, T., Aggarwal, R., Ahn, S. Y., AlMazroa, M. A., Alvarado, M., Anderson, H. R., Anderson, L. M., Andrews, K. G., Atkinson, C., Baddour, L. M., Barker-Collo, S., Bartels, D. H., … Murray, C. J. L. (2012). Global and Regional Mortality from 235 Causes of Death for 20 Age Groups in 1990 and 2010: A Systematic Analysis for the Global Burden of Disease Study 2010. The Lancet, 380(9859), 2095–2128.

Maryani, P. E., Ulfa, E. U., & Rachmawati, E. (2016). Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr) terhadap Kadar Kolesterol Total dan Trigliserida Tikus Hiperlipidemia. E-Jurnal Pustaka Kesehatan, 4(1), 20–26.

Muhartono, P, M. A. Y., Putri, N. T., Sari, T. N., & Oktafany. (2018). Minyak Jelantah Menyebabkan Kerusakan pada Arteri Koronaria, Miokardium, dan Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Sprague dawley. Jurnal Kedokteran Universitas Lampung, 2(2), 129–135.

Na’i, A., Rakanita, Y., & Mulyani, S. (2019). Uji Efek Kombinasi EEDS dan DPW terhadap Penurunan Kadar Kolesterol Total Tikus Putih Jantan Hiperkolesterolemia-Diabetes. Farmakologika Jurnal Farmasi, 16(1), 1–12.

Nelson, R. H. (2013). Hyperlipidemia as a Risk Factor for Cardiovascular Disease. Primary Care - Clinics in Office Practice, 40(1), 195–211.

Nirosha, K., Divya, M., Vamsi, S., & Sadiq, M. (2014). A Review on Hyperlipidemic. International Journal of Novel Trends in Pharmaceutical Science, 4(5), 81–92.

Oguntibeju, O. O. (2019). Type 2 Diabetes Mellitus, Oxidative Stress and Inflammation: Examining the Links. Int J Physiol Pathophysiol Pharmacol, 11(3), 45–63.

Ontawong, A., Boonphang, O., Pasachan, T., Duangjai, A., Pongchaidecha, A., Phatsara, M., Jinakote, M., Amornlerdpison, D., & Srimaroeng, C. (2019). Hepatoprotective Effect of Coffee Pulp Aqueous Extract Combined with Simvastatin Against Hepatic Steatosis in High-fat Diet-induced Obese Rats. Journal of Functional Foods, 54, 568–577.

Phaniendra, A., Jestadi, D. B., & Periyasamy, L. (2015). Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry, 30(1), 11–26.

Rahmiati, B. F., Jauhari, M. T., Ardian, J., & Aini, Q. (2020). Pengaruh Frekuensi Penggorengan dan Penambahan Sari Mengkudu (Morindacitrifolia) terhadap Jumlah Asam Lemak Trans pada Minyak Jelantah. Jurnal Pangan, Gizi, Kesehatan, 1(1), 18–25.

Reiter-Brennan, C., Osei, A. D., Iftekhar Uddin, S. M., Orimoloye, O. A., Obisesan, O. H., Mirbolouk, M., Blaha, M. J., & Dzaye, O. (2020). ACC/AHA Lipid Guidelines: Personalized Care to Prevent Cardiovascular Disease. Cleveland Clinic Journal of Medicine, 87(4), 231–239.

Sakakura, K., Nakano, M., Otsuka, F., Ladich, E., Kolodgie, F. D., & Virmani, R. (2013). Pathophysiology of Atherosclerosis Plaque Progression. Heart Lung and Circulation, 22(6), 399–411.

Sakamoto, K., Nagamatsu, S., Yamamoto, E., Kaikita, K., & Tsujita, K. (2018). Atherosclerotic Coronary Plaque Development Visualized by in vivo Coronary Imaging. Circulation Journal, 82(7), 1727–1734.

Sartika, R. A. D. (2011). Effect of Trans Fatty Acids Intake on Blood Lipid Profile of Workers in East Kalimantan, Indonesia. Malaysian Journal of Nutrition, 17(1), 119–127.

Setiawati, T., Atmomarsono, U., & Dwiloka, B. (2016). Kadar Lemak dan Profil Asam Lemak Jenuh, Asam Lemak tak Jenuh Daging Ayam Broiler dengan Pemberian Pakan Mengandung Tepung Daun Kayambang (Salvinia molesta). Jurnal Teknologi Hasil Pertanian, 9(2), 1–8.

Sitasiwi, A. J., Isdadiyanto, S., & Mardiati, S. M. (2017). The Estradiol 17- β Concentration in Mice After Treated with Ethanolic Leaf Extract of Azadirachta indica (Neem). The 18th International Conference on POsitron Annihilation, 020014, 1–8.

Sopandi, D., Saraswati, T. R., & Yuniwarti, E. Y. W. (2019). Effects of Kersen Juice and Lakum Leaf Extract on Lipid Profile of White Rats with Hyperlipidemia. Biosaintifika Journal of Biology & Biology Education, 11(3), 345–351.

Subermaniam, K., Saad, Q. H. M., Kamisah, Y., & Othman, F. (2015). Effects of Virgin Coconut Oil on the Histomorphometric Parameters in the Aortae and Hearts of Rats Fed with Repeatedly Heated Palm Oil. International Journal of Bioscience, Biochemistry and Bioinformatics, 5(2), 120–131.

Tembe-Fokunang, E. A., Charles, F., Kaba, N., Donatien, G., Michael, A., & Bonaventure, N. (2019). The Potential Pharmacological and Medicinal Properties of Neem (Azadirachta indica A. Juss) in the Drug Development of Phytomedicine. Journal of Complementary and Alternative Medical Research, 7(1), 1–18.

Tomkin, G. H., & Owens, D. (2012). The Chylomicron: Relationship to Atherosclerosis. International Journal of Vascular Medicine, 2012, 1–13.

Urschel, K., & Cicha, I. (2015). TNF-α in The Cardiovascular System: From Physiology to Therapy. International Journal of Interferon, Cytokine and Mediator Research, 7, 9–25.

Widayati, E. (2012). Oksidasi Biologi, Radikal Bebas, dan Antioxidant. Majalah Ilmiah Sultan Agung, 50(128), 1–7.

Yu, X. H., Fu, Y. C., Zhang, D. W., Yin, K., & Tang, C. K. (2013). Foam Cells in Atherosclerosis. Clinica Chimica Acta, 424, 245–252.

Zhang, Q., Saleh, A. S. M., Chen, J., & Shen, Q. (2012). Chemical Alterations Taken Place During Deep-Fat Frying Based on Certain Reaction Products: A review. Chemistry and Physics of Lipids, 165(6), 662–681.

Zhou, Z., Wang, Y., Jiang, Y., Diao, Y., Strappe, P., Prenzler, P., Ayton, J., & Blanchard, C. (2016). Deep-fried Oil Consumption in Rats Impairs Glycerolipid Metabolism, Gut Histology and Microbiota Structure. Lipids in Health and Disease, 15(1), 1–11.

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