Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride

Melycorianda Hubi Ndapamuri(1), Maria Marina Herawati(2), V Irene Meitiniarti(3),

DOI: https://doi.org/10.15294/biosaintifika.v13i1.25954


(1) Faculty of Agriculture and Business, Universitas Kristen Satya Wacana
(2) Faculty of Agriculture and Business, Universitas Kristen Satya Wacana
(3) Faculty of Biology, Universitas Kristen Satya Wacana

Abstract

Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. 

Keywords

Bagasse of Sorghum; Glucose; Hydrolysis; Trichoderma viride

Full Text:

PDF

References

Balai Pengkaji Teknologi Pertanian Bali. (2019). Pasca Panen dan Pengolahan Nira Sorgum Menjadi Gula Cair. Info Teknologi. http://bali.litbang.pertanian.go.id/ind/index.php/info-teknologi/965-pasca-panen-dan-pengolahan-nira-sorgum-menjadi-gula-cair

Bayu, K. M., Rizqiati, H., & Nurwantoro. (2017). Analisis Total Padatan Terlarut, Keasaman, Kadar Lemak, dan Tingkat Viskositas pada Kefir Optima dengan Lama Fermentasi yang Berbeda. Jurnal Teknologi Pangan, 1(2), 33–38.

Goldstein, D., & Mintz, S. (2015). The Oxford companion to sugar and sweets. Oxford University Press.

Hidayanto, E., & Rofiq, A. (2010). Aplikasi Portable Brix Meter untuk Pengukuran Indeks Bias. Berkala Fisika, 13(4), 113-118–118.

Lailah, R., Syauqi, A., & Santoso, H. (2017). Aktivitas Jamur Trichoderma viride pada Substrat Pasta Tepung Kulit Buah Rambutan (Nephelium lappaceum). Bioscience-Tropic, 3(2), 1–7.

Leustean, U., Georgescu, L., & Bahrim, G. (2010). Preliminary study for optimization of enzymatic hydrolysis of waste cellulosic materials. Food Technology, 35(1), 27–33.

Lumoindong, F., & Mamuaja, C. F. (2016). Produksi Gula Cair dari Limbah Selulosik sebagai Alternatif Pengganti Cairan Infus. J. Ilmu Dan Teknologi Pangan, 4(1), 36–43.

Lynd, L. R., Weimer, P. J., Zyl, W. H. Van, & Isak, S. (2002). Microbial Cellulose Utilization : Fundamentals and Biotechnology Microbial Cellulose Utilization : Fundamentals and Biotechnology Downloaded from http://mmbr.asm.org/ on February 6 , 2013 by INDIAN INSTITUTE OF TECHNOLOGY MADRAS. Microbiology and Molecular Biology Reviews, 66(3), 506–577. https://doi.org/10. 1128/MMBR.66.3.506

Mackuľak, T., Prousek, J., Olejníková, P., & Bodík, I. (2010). The Using Enzymes For Degradition of Cellulose Substrate for the production of Biogas. 37th International Conference of Slovak Society of Chemical Engineering, 1407–1412. https://doi. org/10.1007/s10924-010-0258-0

Mandal, A., & Chakrabarty, D. (2011). Isolation of nanocellulose from waste sugarcane bagasse (SCB) and its characterization. Carbohydrate Polymers, 86(3), 1291–1299. https://doi.org/ 10.1016/J.CARBPOL.2011.06.030

Menezes, C. B., Ticona-Benavente, C. A., Tardin, F. D., Cardoso, M. J., Bastos, E. A., Nogueira, D. W., Portugal, A. F., Santos, C. V., & Schaffert, R. E. (2014). Selection indices to identify drought-tolerant grain sorghum cultivars. Genetics and Molecular Research, 13(4), 9817–9827. https://doi.org/10.4238/2014.November.27.9

Pabendon, M. B., Sarungallo, R. S., & Mas’ud, S. (2012). Pemanfaatan Nira Batang, Bagas, dan Biji Sorgum Manis sebagai Bahan Baku Bioetanol. Penelitian Pertanian Tanaman Pangan, 31(3), 180–187. https://doi.org/10.21082/jpptp.v31n3.2012.p%p

Permanasari, A. R., Yulistiani, F., Purnama, R. W., Widjaja, T., & Gunawan, S. (2018). The effect of substrate and enzyme concentration on the glucose syrup production from red sorghum starch by enzymatic hydrolysis. IOP Conference Series: Earth and Environmental Science, 160(1). https://doi.org/10.1088/1755-1315/160/1/012002

Rahayu, M., Samanhudi, & Wartoyo. (2012). Uji Adaptasi Beberapa Varietas Sorgum Manis di Lahan Kering Wilayah Jawa Tengah dan Jawa Timur. Jurnal Caraka Tani, XXVII(1), 53–62.

Saha, B. C. (2004). Lignocellulose Biodegradation and Applications in Biotechnology. January 2004, 2–34. https://doi.org/10.1021/bk-2004-0889.ch001

Sanito, R., Novembrianto, R., & Pandebesie, E. S. (2015). Kajian penentuan fase pertumbuhan kapang dan bakteri selulolitik pada media pertumbuhan. Prosiding Seminar Nasional Manajemen Teknologi XXII, 1–10.

Saparianti, E., Dewanti, T., & Dhoni, S. K. (2012). Hidrolisis Ampas Tebu Menjadi Glukosa Cair Oleh Kapang Tricoderma viride. Jurnal Teknologi Pertanian, 5(1), 1–10.

Sudarmadji, S., B. Haryono, & Suhardi. (1997). Prosedur Analisa Untuk Bahan Makanan dan Pertanian, Liberty, Yogyakarta.

Sulistyani, P., & T.M. Setyowati. (2000). Optimasi pH dan Lama Fermentasi untuk Produk Sirup Glukosa dari Alang-alang oleh Trichoderma viride. Jurusan Teknologi Pangan. fakultas Teknologi Industri. UPN "Veteran". Surabaya.

Suminar, R., Suwarto, & Purnamawati, H. (2017). Determination of N, P, and K Fertilizer Optimum Rates for Sorghum (Sorghum bicolor [L.] Moench). Jurnal Ilmu Pertanian Indonesia, 22(1), 6–12. https://doi.org/10.18343/jipi.22.1.6

Speight, J. (2017). Transformation of inorganic chemicals in the environment in Enviromental Inorganic Chemistry for Engineers. Laramie, Wyoming. CD&Winc., United Stated.

Standar Nasional Indonesia. (1992). Sirup Glukosa. 01-2978-1992. Jakarta.

Thanapimmetha, A., Vuttibunchon, K., Saisriyoot, M., & Srinophakun, P. (2011). Chemical and Microbial Hydrolysis of Sweet Sorghum Bagasse for Ethanol Production. Proceedings of the World Renewable Energy Congress – Sweden, 8–13 May, 2011, Linköping, Sweden, 57, 389–396. https://doi.org/10.3384/ecp11057389

Tnunay, I. M. Y., Chikmawati, T., & Miftahudin. (2019). Morphological Diversity of Local Sorghum Cultival (Sorghum bicolor) of East Nusa Tenggara, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 11(1), 47–54.

Refbacks



Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.