Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

Kharisma Panji Ramadhan(1), Sita Heris Anita(2), Maulida Oktaviani(3), Fahriya Puspita Sari(4), Raden Permana Budi Laksana(5), Oktan Dwi Nurhayat(6), Dede Heri Yuli Yanto(7),

DOI: https://doi.org/10.15294/biosaintifika.v13i1.26148


(1) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(2) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(3) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(4) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(5) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(6) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)
(7) Research Center For Biomaterials, Indonesian Institute of Sciences (LIPI)

Abstract

Water pollution by dyes represents from dyestuff industry becomes an environmental concern. Finding new isolates capable of decolorizing these dyes is important. The study aimed to assess the new isolates of white-rot fungi (WRF) as decolorizing agent of anthraquinone and azo dyes. Decolorization assay were conducted in Agar plates and liquid medium. During the decolorization, laccase activities produced by the fungal strains were analyzed. Identification of the fungal strains were investigated using molecular DNA analysis. The results showed that isolates M3, H18, and GP1 were able to decolorize anthraquinone and azo dyes in Agar and liquid medium. Based on DNA analysis, isolates M3, H18, and GP1 have the similarity to Trametes sanguinea, Trametes polyzona, and Neofomitella guangxiensis, respectively. Among the fungi, T. polyzona H18 exhibited high decolorization ability (70–90%) to the dyes (100 mg/L) after 96-hours incubation. Laccase activity was fluctuated during the reactions with tendency to increase at the beginning until its peak, then decreased at the end of incubation. This study demonstrated the potential of the new isolates from Indonesia to decolorize anthraquinone and azo dyes. The results of the study can provide an alteranative for bioremediation agents of contaminated water by synthetic dyes.

Keywords

Decolorization; Laccase; Textile Wastewater; Trametes polyzona; White-Rot Fungi

Full Text:

PDF

References

Antošová, Z., Herkommerová, K., Pichová, I., & Sychrová, H. (2018). Efficient secretion of three fungal laccases from Saccharomyces cerevisiae and their potential for decolorization of textile industry effluent—A comparative study. Biotechnology Progress, 34(1), 69–80.

Bagewadi, Z. K., Mulla, S. I., & Ninnekar, H. Z. (2017). Purification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization. Journal of Genetic Engineering and Biotechnology, 15(1), 139–150.

Bibi, I., Javed, S., Ata, S., Majid, F., Kamal, S., Sultan, M., Jalani, K., Umair, M., Khan, M.I., Iqbal, M. (2019). Biodegradation of synthetic orange G dye by Plearotus sojar-caju with Punica granatum peal as natural mediator. Biocatalysis and Agricultural Biotechnology, 22, 101420.

Cao, X., Wang, H., Zhang, S., Nishimura, O., & Li, X. (2018). Azo dye degradation pathway and bacterial community structure in biofilm electrode reactors. Chemosphere, 208, 219–225.

Chairin, T., Nitheranont, T., Watanabe, A., Asada, Y., Khanongnuch, C., & Lumyong, S. (2013). Biodegradation of bisphenol A and decolorization of synthetic dyes by laccase from white-rot fungus, Trametes polyzona. Applied Biochemistry and Biotechnology, 169(2), 539–545.

Cui, B. K., Li, H. J., Ji, X., Zhou, J. L., Song, J., Si, J., Yang, Z.L., Dai, Y. C. (2019). Species diversity, taxonomy and phylogeny of Polyporaceae (Basidiomycota) in China. Fungal Diversity, 97, 137–392.

Hefnawy, M. A., Gharieb, M. M., Shaaban, M. T., & Soliman, A. M. (2017). Optimization of culture condition for enhanced decolorization of Direct blue dye by Aspergillus flavus and Penicillium canescens. Journal of Applied Pharmaceutical Science, 7(2), 083–092.

Ji, X., Wu, D. M., Song, C. G., Liu, S., Si, J., & Cui, B. K. (2019). Two new Neofomitella species (Polyporaceae, Basidiomycota) based on morphological and molecular evidence. Mycological Progress, 18(4), 593–602.

Kant, R. (2012). Textile dyeing industry an environmental hazard. Natural Science, 4(01), 22–26.

Kaur, B., Kumar, B., Garg, N., & Kaur, N. (2015). Statistical optimization of conditions for decolorization of synthetic dyes by Cordyceps militaris MTCC 3936 using RSM. BioMed Research International, 2015, 1–17.

Lavanya, C., Dhankar, R., Chhikara, S., Sheoran, S. (2016). Degradation of toxic dyes- A review. International Journal of Pure & Applied Bioscience, 4(5), 81–89.

Levin, L., Melignani, E., & Ramos, A. M. (2010). Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates. Bioresource Technology, 101(12), 4554–4563.

Li, H. hong, Wang, Y. tao, Wang, Y., Wang, H. xia, Sun, K. kai, & Lu, Z. mei. (2019). Bacterial degradation of anthraquinone dyes. Journal of Zhejiang University: Science B, 20(6), 528–540.

Ling, Z. R., Wang, S. S., Zhu, M. J., Ning, Y. J., Wang, S. N., Li, B., Yang, A.Z., Zhang, G.Q., Zhao, X. M. (2015). An extracellular laccase with potent dye decolorizing ability from white-rot fungus Trametes sp. LAC-01. International Journal of Biological Macromolecules, 81, 785–793.

Lueangjaroenkit, P., Teerapatsakul, C., & Chitradon, L. (2018). Morphological characteristic regulation of ligninolytic enzyme produced by Trametes polyzona. Mycobiology, 46(4), 396–406.

Madhushika, H. G., Ariyadasa, T. U., & Gunawardena, S. H. P. (2020). Biological decolourization of textile industry wastewater by a developed bacterial consortium. Water Science and Technology, 80(10), 1910–1918.

Manavalan, T., Manavalan, A., Thangavelu, K. P., & Heese, K. (2013). Characterization of optimized production, purification and application of laccase from Ganoderma lucidum. Biochemical Engineering Journal, 70, 106–114.

Matjuškova, N., Okmane, L., Zala, D., Rozenfelde, L., Puke, M., Kruma, I., Vedernikovs, N., Rapoport, A. (2017). Effect of lignin-containing media on growth of medicinal mushroom lentinula edodes. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 71(1), 38–42.

Moreira-Neto, S. L., Mussatto, S. I., Machado, K. M. G., & Milagres, A. M. F. (2013). Decolorization of salt-alkaline effluent with industrial reactive dyes by laccase-producing basidiomycetes strains. Letters in Applied Microbiology, 56(4), 283–290.

Padmapriya, G., Manikandan, A., Krishnasamy, V., Jaganathan, S. K., & Antony, S. A. (2016). Spinel NixZn1-xFe2O4 (0.0 ≤ x ≤ 1.0) nano-photocatalysts: Synthesis, characterization and photocatalytic degradation of methylene blue dye. Journal of Molecular Structure, 1119, 39–47.

Patel, H., Gupte, S., Gahlout, M., & Gupte, A. (2014). Purification and characterization of an extracellular laccase from solid-state culture of Pleurotus ostreatus HP-1. 3 Biotech, 4(1), 77–84.

Risdianto, H., Sofianti, E., Suhardi, S. H., & Setiadi, T. (2012). Optimisation of laccase production using white-rot fungi and agriculture wastes in solid state fermentation. ITB Journal of Engineering Science, 44 B(2), 93–105.

Robinson, P. K. (2015). Enzymes: principles and biotechnological applications. Essays in Biochemistry, 59, 1–41.

Å ekuljica, N. Ž., Prlainović, N. Ž., Stefanović, A. B., Žuža, M. G., ÄŒiÄkarić, D. Z., Mijin, D. Ž., & Knežević-Jugović, Z. D. (2015). Decolorization of anthraquinonic dyes from textile effluent using horseradish peroxidase: Optimization and kinetic study. The Scientific World Journal, 2015, 1–12.

Sing, N. N., Husaini, A., Zulkharnain, A., & Roslan, H. A. (2017). Decolourisation capabilities of ligninolytic enzymes produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and other azo dyes. BioMed Research International, 2017, 1–8.

Solís, M., Solís, A., Pérez, H. I., Manjarrez, N., & Flores, M. (2012). Microbial decolouration of azo dyes: A review. Process Biochemistry, 47(12), 1723–1748.

Strong, P. J., & Claus, H. (2011). Laccase: A review of its past and its future in bioremediation. Critical Reviews in Environmental Science and Technology, 41(4), 373–434.

Yanto, D. H. Y., Auliana, N., Anita, S. H., & Watanabe, T. (2019). Decolorization of synthetic textile dyes by laccase from newly isolated Trametes hirsuta EDN084 mediated by violuric acid. IOP Conference Series: Earth and Environmental Science, 374(1), 012005.

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.