Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.
(1) Graduate School, IPB University
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
(3) Tropical Biopharmaca Research Center, IPB University
Abstract
Antiobesity medication is available as therapeutic compounds that can reduce fat digestion by the inhibition of pancreatic lipase. Actinobacteria have the potency as source of bioactive compounds with various biological function including as pancreatic lipase inhibitor. However, the potency of endophytic actinobacteria from Rhododendron spp. as source of pancreatic lipase inhibitor producer has not been reported yet. The aim of this study was to examine the potential of pancreatic lipase inhibitory activity of 23 endophytic actinobacteria from Rhododendron spp.; to characterize their colony based on morphology and molecular analysis. Screening test of pancreatic lipase inhibitor was conducted using the supernatant of endophytic actinobacteria, lipase pancreatic porcine (L3126) and p-nitrophenyl butyrate. The supernatant of selected isolates was extracted using ethyl acetate. The result showed that various inhibitory activities ranging between 0.00 until 91.69%. There were 11 out of 23 isolates that have potential as pancreatic inhibitor. Amongst them, the extract of four selected isolates, i.e. RZP 1.3, RSSB 3.2, RSS 2.1, and RJB F3.2 demonstrated inhibitory percentage of more than 80%. The RJB F3.2 extract showed to have IC50 value by 431.48 µg mL-1 compared to control, i.e. Xenical (89.07 µg mL-1). Phytochemical analysis exhibited that the extract of the selected isolates contained alkaloid which may function as pancreatic lipase inhibitor. Based on the morphological character, the selected isolates have various morphological colonies and 16S rRNA gene sequence revealed the sequence homology to Streptomyces spp. The data clearly indicate that endophytic actinobacteria from Rhododendron spp. have potency as pancreatic lipase inhibitor producer and further studies could be explored for the development of antiobesity agent.
Keywords
Full Text:
PDFReferences
Bate, N., Butler A.R., Smith, I.P., & Coundlife E. (2000). The mycarose-biosynthetic genes of Streptomyces fradiae producer of tylosin. Microbiology, 146, 139-146. https://doi.org/10.1099/00221287-146-1-139
Chedda, U., Kaikini. A., Bagle, S., Seervi, M., & Sathaye S. (2016). In vitro pancreatic lipase inhibition potential of commonly used Indian spices. IOSR Journal of Pharmacy, 6(10), 10-13.
Dechakhampu, A., & Wongchum, N. (2015). Screening for anti-pancreatic lipase properties of 28 traditional Thai medicinal herb. Asian Pacific Journal of Tropical Biomedicine, 5(12), 1042-1045. http://dx.doi.org/10.1016/j.apjtb.2015.09.012
Dehnad, A. R., Laleh, P. Y., Rouhollah, B., Ahad, M., Samad, A. S., Ali, R. M., Sevda, G., & Rahib, A. (2010). Investigation antibacterial activity of Streptomyces isolates from soil samples, West of Iran. African Journal of Microbiology Research, 04(16), 1685-1693. https://doi.org/10.5897/AJMR.9000429
Flärdh, K., & Buttner, M. (2009). Streptomyces morphogenetics: dissecting differentiation in a filamentous bacterium. Nature Review, 7, 3649. https://doi.org/10.1038/nrmicro1968
Fitri, L., Meryandini, A., & Lestari, Y. (2017). Diversity of endophytic actinobacteria isolated from medical plants and their potency as pancreatic lipase inhibitor. Biodiversitas, 18(03), 857-863. https://doi.org/10.13057/biodiv/d180301
Fitriandini, R., Budiarti, S., & Lestari, Y. (2017). Endophytic Actinobacteria from Rhododendron spp. as an antibacterial agent. Biosaintifika, 9(3), 600-607. https://doi.org/10.15294/biosaintifika.v9i3.10323
Gupta, M., Saxena, S., & Goyal, D. (2014). Potential pancreatic lipase inhibitory activity of an endophytic Penicillium species. Journal of Enzyme Inhibition and Medicinal Chemistry, 01-07. https://doi.org/10.3109/14756366.2013.871007
Harborne, J.D. (1987). Phytochemical methods. London (UK): Chapman & Hall.
Hofbauer, K.G. (2002). Molecular pathways to obesity. International Journal of Obesity, 26(2), 18-27. https://doi.org/10.1038/sj.ijo.0802124
Iswantini, D., Silitonga R.F., Martatilofa, E., & Darusman, L.K. (2011). Zingiber cassumunar, Guazuma ulmifolia and Murraya paniculata extracts as antiobesity; in vitro inhibitory effect on pancreatic lipase activity. HAYATI Journal of Biosciences, 18(1), 6-10. https:doi.org/110.4308/hjb.18.1.6
Karu, N., Reifen, R., & Kerem, Z. (2007). Weight gain reduction in mice fed Panax ginseng saponin, a pancreatic lipase inhibitor. Journal of Agricultural and Food Chemistry, 55, 2824-2828. https:doi.org/10.10211/jf0628025
Kekuda, P., & Onkarappa R. (2014). Antioxidant, anthelmintic and enzyme inhibitory potential of Streptomyces variablis strain PO-178 isolated from Western Ghat soil of Agumbe, Karnataka, India. Journal of Biological and Scientific Opinion. 02(02), 170-176. doi:10.7897/2321-6328.02239
Kekuda, P., Shobha, K.S., Onkarappa, R. (2011). Pancreatic lipase inhibitor and cytotoxic potential of a Streptomyces species isolated from Western Ghat soil, Agumbe, Karnataka. International Journal of Pharmaceutical & Biological Archive, 02(03), 932-837.
Laidi, R.L., Kansoh, A.L., Elshafei, A.M., & Cheikh, B. (2006). Taxonomy, identification and biological activities of a novel isolate of S. tendae. Arab Journal of Biotechnology, 9(3), 427-436.
Li, F., Li, W., Fu, H., Zhang, Q., & Koike, K. (2007). Pancreatic lipase-inhibiting triterpenoid saponin from fruits of Achanthopanax senticosus. Cemical and Pharmaceutical Bulletin, 55(07),1087-108. http://doi.org/10.1248/cpb.55.1087
Madigan, M.T., Martinko, J.M., Bender, K.S., Buckley, D.H.. & Stahl, D.A. (2015). Brock Biology of Microorganism. Ed. 14. New York (US): Pearson.
Martina, K., Jan, K., Tamas, F., Ladislav, C., Marek, O., Geneviave, L.G., Yvan, M.L., & Marketa, S.M. (2008). Development of a 16S rRNA gene-based prototype microarray for the detection of selected actinomycetes genera. Antonie Van Leuwenhoek, 94(03), 439-453. https: doi.org/10.1007/s10482-008-9261-z
Mukerjee, M. (2003). Human digestive and metabolic lipases a brief reviews. Journal of Molecular Catalysis B: Enzymatic, 22, 369-376. http://doi.org/10.1016/S1381-1177(03)00052-3
Ono, Y., Hattori, E., Fukaya, Y., Imai, S., & Ohizumi, Y. (2006). Antiobesity effect of Nelumbo nucifera leaves extract in mice and rats. Journal of Ethnopharmacology, 106, 238-244. https://doi.org/10.1016/j.jep.2005.12.036
Prakash, O., Verma, M., Sharma, P., Kumar, M., Kumari, K., Singh, A., Kumari H., Jit, S., Gupta, S. K., Khanna, M., Lal, R. (2007). Polyphasic approach of bacterial classification- an overview of recent advances. Indian Journal of Microbiology, 47, 98-108. https://doi.org/10.1007/s12088-007-0022-x
Pujiyanto, S., Lestari, Y., Suwanto, A., Budiarti, S., & Darusman, L.K. (2012). Alpha-glucosidase inhibitor activity and characterization of endophytic actinomycetes isolated from some Indonesia diabetic medical plant. International Journal of Pharmacy Pharmaceutical Sciences, 4(1), 327-333.
Ramasamy, D., Mishra, A.K., Lagier, J.C., Padhamabhan, R., Rossi, M., Sentausa, E., Raoult, D., & Fournier, P.E. (2014). A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. International Journal of Systematic Evolutionary Microbiology, 64(1), 384-391. https://doi.org/10.1099/ijs.0.057091-0
Singh, G., Venkata, K.B., & Ravi, K.K. (2017). Characterization and kinetics of lipase inhibitory activity of Streptomyces tendae. International Journal of Pharmacy and Pharmaceutical Sciences, 8(12), 270-274. http://dx.doi.org/10.22376/ijpbs.2017.8.2.b270-274
Stackebrandt, E., et al. (2002). Report of the ad hoc committee for the re-evolution of the species definition in bacteriology. International Journal of Systematic Evolutionary Microbiology, 52, 1043-1047. http://doi.org/10.1099/00207713-52-3-1043
Strobel, G., & Daisy, B. (2003). Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews, 67(04), 491-502. http://dx.doi.org/10.1128/MMBR.67.4.491-502.2003
Sukhdev, S., & Singh, K.S. (2013). Therapeutic role of phytomedicines on obesity: importance of herbal pancreatic lipase inhibitors. International Research Journal of Medical Sciences, 1(9), 15-26.
Tan, R.X., & Zou, W.X. (2001). Endophytes: a rich source of functional metabolites. Natural Product Reports, 18, 448-459. https://doi.org/10.1039/b100918o
Verma, N., Singh, A.P., Amresh, G., Sahu, P.K., & Rao, C.V. (2010). Anti-inflammatory and anti-nociceptive activity of Rhododendron arboreum. Journal of Pharmacy Research, 03(06), 1376-1380.
Verma. N., Amresh, G., Sahu, P.K., Rao, C.V., & Singh, A.P. (2012). Antihyperglycemic and antihyperlipdemia activity of ethyl acetate fraction of Rhododendron arboreum Smith. flowers in streptomycin induce diabetic rats and its role in regulating carbohydrate metabolism. Asian Pacific Journal of Tropical Biomedicine, 2(9), 696-701. https://doi.org/10.1016/S2221-1691(12)60212-3
Weigle D.S. (2003). Pharmacological therapy of obesity: past, present and future. The Journal of Clinical Endocrinology & Metabolism, 88(6), 2462-2469. https://doi.org/10.1210/jc.2003-030151
Whitman,W.D., Michael, G., Peter, K., Han, J.B., Martha, E.T., Wolfgang, L., Kenichro, S. (2012). Bergey’s manual of systematic bacteriology. New York (US): Springer.
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.