Kajian Efektifitas Rhizoctonia SP Mikoriza Dataran Rendah dan Sedang pada Tingkat Keparahan Penyakit (Dsi) Anggrek Phalaenopsis amabilis terhadap Fusarium sp.
(1) 
Abstract
Anggrek Phalaenopsis amabilis atau yang lebih dikenal sebagai anggrek bulan merupakan plasma nutfah dan tersebar diberbagai tempat di Indonesia. Keberadaan anggrek ini semakin berkurang karena serangan jamur patogen. Fusarium sp. merupakan patogen yang paling banyak menyerang anggrek P. amabilis (Chung et al., 2011) dibandingkan dengan jamur patogen lainnya. Serangan Fusarium sp. akan menyebabkan daun bewarna kuning dan membusuk. Pada penelitian ini dilakukan penggunaan Rhizoctonia mikoriza yang diisolasi dari P. amabilisdi wilayah Sleman dan Surakarta menurut metode Bayman et al. (Otero, 2002), untuk mengetahui perbedaan efektifitasnya didalam menghambat pertumbuhan Fusarium sp. yang diisolasi menurut metode Barnet dan Hunter (1972). Penelitian menggunakan metoda RAL dengan 2 perlakuan dan 6 ulangan selama 8 bulan, masing-masing diberi dan tanpa Rhizoctonia mikoriza dan Fusarium sp. Hasil penelitian menunjukkan terjadi peningkatan pertumbuhan vegetatif pada anggrek P. amabilis yang diprainokulasi Rhizoctonia mikoriza yang diisolasi dari P. amabilis di Surakarta (M2) terhadap Fusarium sp. Tingkat keparahan penyakit (DSI) anggrek P. amabilis terhadap Fusarium sp. yang diprainokulasi Rhizoctonia mikoriza dari P. amabilis di Sleman lebih tinggi dibanding dari Surakarta
Phalaenopsis amabilis or better known as orchids in the germplasm and scattered in various places in Indonesia. The existence of this orchid wane since the attacks of pathogenic fungi. Fusarium sp. is the most attacking pathogen P. amabilis orchid (Chung et al., 2011) compared to other pathogenic fungi. Fusarium sp. attack will cause the leaves to rot and yellow colored. In this study the use of mycorrhizal Rhizoctonia isolated P. amabilis from Sleman and Surakarta in the green house UTP to determine differences in effectiveness in inhibiting the growth of Fusarium sp. Research using the method of RAL with 6 replications for 8 months.The results showed an increase in the vegetative growth of P. amabilis orchid mycorrhiza which prainoculated with Rhizoctonia isolated from P. amabilis in Surakarta (M2) against Fusarium sp. The severity of disease (DSI) P. amabilis orchid against Fusarium sp. which prainoculated with mycorrhizal Rhizoctonia from P. amabilis in Sleman higher than from Surakarta
Keywords
Full Text:
PDFReferences
Aberoumand, A. & Deokule, S.S. (2008). Comparison of phenolic compounds of some edible plants of Iran and India. Pakistan Journal of Nutrition 7 : 582 – 585.
Agrios, G. N. (2005). Plant Pathology. 4th ed. Academic Press. New York. 922 p.
Barnett, H. L. & B. B. Hunter. (1972). Illustrated Genera of Imperfect Fungi, 3rd Edition. Burgess Publishing Company. Minneapolis, Minnesota. 241p.
Cardoso, J. E. & E. Echandi. (1987). Nature of protection of bean seedling from Rhizoctonia root rot by a binucleate Rhizoctonia-like fungus. Phytopathology 77 : 1548 – 1551.
Carling, D.E., E. J. Pope, K. A. Brainard and D. A. Carter. (1999). Characterization of mycorrhiza isolates of Rhizoctonia solani from an orchid, including AG-12, a new anastomosis group. Phytopathology 89 : 942 – 946.
Chung J. W., L. W. Chen, J. H. Huang, H. C. Huang, and W. H. Chung. (2011). A new ‘ forma spesialis’ of Fusarium solani causing leaf yellowing of Phalaenopsis, Plant Pathology, 60, 244-252.
Harris, A.R., D.A. Schisler, S.M. Neate and M.H. Ryder. (1993). Suppression of damping-off caused by Rhizoctonia solani, and growth promotion, in bedding plants by binucleate Rhizoctonia spp. Soil Biology Biochemistry 26 : 263 – 268.
Hayakawa, S., Y. Uetake and A. Ogoshi. (1999). Identification of symbiotic rhizoctonias from naturally occuring protocorms and roots of Dactylorhiza aristata (orchidaceae). Journal of Faculty Agriculture Hokkaido University 6 : 129 – 141.
Latifah Z, Nur Hayati MZ, Baharuddin S, Masiah Z. (2009). Identification and Pathogenicity of Fusarium spesies associated with root rot and stem rot of Dendrobium, Asian Journal of Plant Pathology 3, 14-21.
Ogoshi, A., M. Oniki, T. Araki and T. Ui. (1983). Studies on the anastomosis groups of binucleate Rhizoctonia and their perfect states. Journal of Faculty Agriculture Hokkaido University 61 : 244 – 260.
Otero, J. T., J. D. Ackerman and P. Bayman. (2002). Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids. American Journal of Botany 89 : 1852-1858.
Senthilkumar, S., K. V. Krishnamurthy, S. J. Britto and D. I. Arockiasamy. (2000). Visualization of orchid mycorrhiza fungal structures with fluorescence dye using epifluorescence microscopy. Current Science 79: 1527-1528.
Smith, S.E. and D. J. Read. (2008). Mycorrhizal Symbiosis, 3rd Edition. Academic Press. New York. 805 p.
Sneh, B., E. Yamoah and A. Stewart. (2004). Hypovirulent Rhizoctonia spp. isolates from New Zealand soils protect radish seedlings against damping-off caused by F. oxysporum. New Zealand Plant Protection 57 : 54 – 58.
Soelistijono. (2013). Pemanfaatan Rhizoctonia Mikoriza Untuk Pengendalian Penyakit Busuk Akar Pada Tanaman Anggrek Spathoglottis plicata. Disertasi. Program Pasca Sarjana Universitas Gadjah Mada. Yogyakarta. 111 h. (tidak dipublikasikan).
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.