The application of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM in the degradation of batik waste

Yohanes Subowo(1), Suliasih Suliasih(2), Sri Widawati(3),

DOI: https://doi.org/10.15294/biosaintifika.v15i3.41987


(1) The Center Research of Microbiology Aplication – BRIN. Jl. Raya Bogor KM 46 Cibinong Bogor, Indonesia
(2) The Center Research of Microbiology Aplication – BRIN. Jl. Raya Bogor KM 46 Cibinong Bogor, Indonesia
(3) The Center Research of Microbiology Aplication – BRIN. Jl. Raya Bogor KM 46 Cibinong Bogor, Indonesia

Abstract

The batik industry in Indonesia produces batik waste which pollutes the environment. This waste can be degraded using laccase-producing microorganisms. The microorganisms used in the research were the fungus Rigidoporus sp J12 and the bacteria Stenotrophomonas maltophilia BM. This research aims to determine the ability of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM and their consortium in producing laccase, observing their ability to degrade Poly R-478 which is an indicator of phenoloxidase activity and batik waste. Microorganisms are grown in growth media and then placed in media containing Poly R-478 or batik waste. Inducers are added to increase laccase activity. The inducers used were 15 g/L sucrose, 200 µM CuSO4 and 40 mM veratryl alcohol. The results showed that Rigidoporus sp J12 and Stenotrophomonas maltophilia BM produced laccase in PDB and NA media. The highest laccase activity was found in the enzyme produced by Rigidoporus sp J12 in PDB media at a temperature of 40°C, media pH 6.0 and the addition of sucrose. Rigidoporus sp J12 degraded batik waste by 39.38% and increased by 2.12 times after adding sucrose and incubation for 15 days. These bacteria and fungi can be used to degrade batik waste in order to prevent environmental pollution. Using the fungus Rigidoporus sp J12 purely is more profitable than using it with S. maltophilia BM bacteria.

Keywords

batik waste; degradation; laccase; Rigidoporus; Stenotrophomonas maltophilia

Full Text:

PDF

References

Alaya, V., Kodi, R.K., Ninganna, E., Gowda, B. & Shivanna, M.B. (2021). Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium. Bulletin of the National Research Centre, 45(81), 13 pp.

Apriyani, N. (2018). Batik Industry: Liquid Waste Content and Processing Methods. Media Ilmiah Teknik Lingkungan, 3(1), 21-29.

Dao, A.T.N., Smits, M., Dang, H.T.C., Brouwer, A., de Boer, T.E. (2021). Elucidating fungal Rigidoporus species FMD21 lignin-modifying enzyme genes and 2,3,7,8-tetrachlorodibenzo-p-dioxin degradation by laccase isozymes. Enzyme and Microbial Technology, 147, 9 pp.

Esmaeili, A. & Fazeli, S. (2012). Investigation of biodegradation of an aromatic compound in industrial waste water. Rom J. Biochem., 49(2), 153–161.

Eichlerova, I. & Baldrian, P. (2020). Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes. J. Fungi, 6(301), 22 pp.

Galai, S., Touhami, Y., & Marzouki, M.N. (2012). Response surface methodology applied to laccases activities exhibited by Stenotrophomonas maltophilia AAP56 in different growth conditions. Bioresources, 7(1), 706-726.

Galai, S., Korri-Youssoufib, H., & Marzouki, M.N. (2014). Characterization of yellow bacterial laccase Sm Lac/role of redox mediators in azo dye decolorization. J. Chem Technol Biotechnol, 89, 1741–1750.

Glenn, J. K., & Gold, M. H. (1983). Decolorization of several polymeric dyes by the lignin–degrading Basidiomycetes Phanerochaete chrysosporium. Appl. Environ Microbiol. 45(6), 1741-1747.

Lade, H., Kadam, A., Paul, D., & Govindwar, S. (2016). Exploring the potential of fungal-bacterial consortium for low-cost biodegradation and detoxification of textile effluent. Archives of Environmental Protection, 42(4), 12-21.

Mawad, A.M.M., Hesham, A.E.L., Yousef, N.M.H., Shoreit, A.A.M., Gathergood, N., & Gupta, V.K. (2020). Role of Bacterial-Fungal Consortium for Enhancement in the Degradation of Industrial Dyes. Current Genomics, 21, 283-294.

Mazlan, S.Z.& Haanifah, S.A. (2017). Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres. International Journal of Polymer Science, 8 pp.

Melloul, E., Luiggi, S., Anais, L., Arne, P., Costa, J.M., Fihman, V., Briard, B., Dannaoui, E., Guillot, J., Decousser, J.W., Beauvais, A., Botterel, F. (2016). Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm. PLOS ONE, 18 pp.

Mishra, S. & Srivastava, S.K. (2016). Production of Extracellular laccase from Bacterial Strain Bacillus subtilis MTCC 1039 using different Parameter. Biosci., Biotech. Res. Asia, 13(3), 1645-1650.

Nuriyanto,L.K. (2022). Preservation of the Batik Industry in Indonesia as Part of the National Identity. Int. J. Sci. Appl. Sci., 6(2), 18 pp.

Olukanni, O.D., Osuntoki, A.A., Awotula, A.O., Kalyani, D.C., Gbenle, G.O., & Govindwar, S.P. (2013). Decolorization of Dyehouse Effluent and Biodegradation of Congo Red by Bacillus thuringiensis RUN1. J. Microbiol. Biotechnol., 23(6), 843–849.

Papinutti, V.L., Diorio, L.A., & Forchiassin, F. (2003). Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran. J. Ind. Microbiol. Biotechnol. 30, 157-160

Pertiwi, S., Dewi, R.S., Sari, A.A. (2020). Decolorization of Indigosol Blue Dye Using Trametes versicolor F200 and Aspergillus sp. Bioeksakta, 2(2), 222-226.

Poornima, R., Sneha, U., Sridhar, S. (2014). Optimization of Enzymatic Decolourization of Amido black by Laccase from Rigidoporus ulmarius using Response Surface Methodology. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT), 8(4), 64-69.

Rajeswari, K., Subashkumar, R., Vijayaraman, K. (2013). Decolorization and Degradation of Textile Dyes by Stenotrophomonas maltophilia RSV-2. International Journal of Environmental Bioremediation & Biodegradation, 1(2), 60-65.

Risdianto, H., Sofianti, E., Suhardi, S.H., & Setiadi, T. (2012). Optimization of Laccase Production using White Rot Fungi and Agricultural Wastes in Solid-State Fermentation. ITB J. Eng. Sci., 44 (2), 93-105.

Shin, D.Y., Lee, S.M., Jang, Y., Lee, J., Lee, C.M., Cho, E.M., & Seo, Y.R. (2023). Adverse Human Health Effects of Chromium by Exposure Route: A Comprehensive Review Based on Toxicogenomic Approach. Int. J. Mol. Sci., 24, 12 pp.

Sridhar, S., Chinnathambi, V., Arumugam, P., Suresh, P.K. (2013). In Silico and in Vitro Physicochemical Screening of Rigidoporus sp. Crude Laccase-assisted Decolorization of Synthetic Dyes—Approaches for a Cost-effective Enzyme-based Remediation Methodology. Appl. Biochem. Biotechnol, 12 pp.

Subowo, Y.B & Sugiharto, A. (2021). The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME. Biosaintifika, 13(2), 243-249.

Strong, P.J. (2011). Improved Laccase Production by Trametes pubescens MB89 in Distillery Wastewaters. Enzyme Research, 8 pp.

Umar, A.& Ahmed, S. (2022). Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship. Sci. Rep., 12(1), 13 pp.

Unuofin, J.O. (2020). Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: From dye decolourization to denim bioscouring. Biotechnology Reports, 25, 10 pp.

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.