The Growth of Tagetes patula Linn. and Its Ability to Reduce Cr(VI) with the Addition of Microbacterium sp. SpR3

Firdiana Novellasari(1), V. Irene Meitiniarti(2), Mahmoud F. Seleiman(3), Sri Kasmiyati(4),

DOI: https://doi.org/10.15294/biosaintifika.v15i3.44957


(1) Faculty of Biology, Universitas Kristen Satya Wacana, Jl. Diponegoro No.52-60, Salatiga, Central Java 50711, Indonesia
(2) Faculty of Biology, Universitas Kristen Satya Wacana, Jl. Diponegoro No.52-60, Salatiga, Central Java 50711, Indonesia
(3) Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
(4) Faculty of Biology, Universitas Kristen Satya Wacana, Jl. Diponegoro No.52-60, Salatiga, Central Java 50711, Indonesia

Abstract

Cr(VI) is a heavy metal that has the potential to become a soil pollutant and has an impact on organisms. The contamination caused by Cr(VI) could be alternatively treated with bioremediation techniques. The current study aimed to determine the most potential combination of Tagetes patula Linn. and Microbacterium sp. strain SpR3 for remediation of soil Cr(VI) contamination based on growth of T. patula. The application of SpR3 applied at the 1st day (T0) and 20th day (T20) with 10 g (M10), 30 g (M30), 50 g (M50) of bacterial inoculum to T. patula grown under Cr(VI; 100 mg/L). The results showed that T0M50 treatment resulted in the highest values of growth traits of T. patula grown under Cr(VI) metal stress. The highest BC value (0.36) was obtained from plants treated with T2M10 and T2M50, while the highest TF value (0.08) obtained from plants treated with T0M50. BC value <1 means that the combination of T. patula and SpR3 bacteria for heavy metal Cr(VI) can be classified as an excluder and the TF value <1 means that the combination can act as a phytostabilization in handling Cr(VI) contamination. In conclusion, the application of SpR3 using T0M50 can enhance the growth of Tagetes patula Linn. grown under Cr(VI) stress condition. The outcome of the study are expected to advancement in the application of rhizobacterial and plant combined system in the bioremediation of soil Cr(VI) contaminated.

Keywords

Bioremediation, Cr(VI), Microbacteriumsp. strain SpR3, Tagetes patula

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References

Ahemad M. (2015). Enhancing phytoremediation of chromium-stressed soil through plant-growth-promoting bacteria. Journal of Genetic Engineering and Biotechnology 13:51-58.

Aravindhan S, Jawaharlal M, Thamaraiselvi SP, & Davamani V. (2019). Effect of heavy metals on morphological and flowering parameters of african marigold (Tagetes erecta). International Journal of Chemical Studies 7(3): 4321-4323.

Awan B, Sabeen M, Shaheen S, Mahmood Q, Ebadi A, Toughani M. (2020). Phytoremediation of zinc contaminated water by marigold (Tagetes minuta L). Central Asian Journal of Environmental Science and Technology Innovation 1(3):150-158.

Bader N, Alsharif E, Nassib M, Alshelmani N, & Alalem A. (2019). Phytoremediation potential of Suaeda vera for some heavy metals in roadside soil in Benghazi, Libya. Asian Journal of Green Chemistry 3: 82-90.

Biswal B, Singh SK, Patra A, & Mohapatra KK. (2021). Evaluation of phytoremediation capability of french marigold (Tagetes patula) and african marigold (Tagetes erecta) under heavy metals contaminated soils. International Journal of Phytoremediation DOI: 10.1080/15226514.2021.1985960.

Briffa J, Sinagra E, Blundell R. (2020). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon 6:e04691.

Chaturvedi N, Ahmed MdJ, & Dhal NK. (2014). Effect of iron ore tailings on growth and physiological activities of Tagetes patula L. Journal Soils Sediments 14:721-730.

Choppala G, Bolan N, & Park JH. (2013). Chromium contamination and its risk management in complex environmental settings. Advances in Agronomy 120:129-172.

Elahi A, Ajaz M, Rehman A, Vuilleumier S, Khan Z, & Hussain SZ. (2019). Isolation, characterization, and multiple heavy metal-resistant and hexavalent chromium-reducing Microbacterium testaceum B-HS2 from tannery effluent. Journal of King Saud University-Science 31(4): 1437–1444.

Focardi S, Pepi M, & Focarsi SE. (2013). Microbial reduction of hexavalent chromium as a mechanism of detoxification and possible bioremediation application. Intech Biodegradation Life of Science p 321-347.

Gheju M, Balcu I, & Ciopec M. (2009). Analysis of hexavalent chromium uptake by plant in polluted soils. Ovidius University Annals of Chemistry 20 (1): 127-131.

Gomes MAdC, Hauser-Davis RA, Suzuki MS, & Vitoria AP. (2017). Plant chromium uptake and transport, physiological effects and recent advances in molecular investigations. Ecotoxicology and Environmental Safety 140: 55-64.

Joutey NT, Sayel H, Bahafid W, & Ghachtouli NE. (2015). Mechanisms of hexavalent chromium resistance and removal by microorganisms. Springer International Publishing Switzerland 233: 45-69.

Kumar V, Omar R, & Kaistha SD. (2016). Phytoremediation enhaced with concurrent microbial plant growth promotion and hexavalent chromium bioreduction. Journal of Bacteriology & Mycology 2(6):166-168.

Learman DR, Ahmad Z, Brookshier A, Henson MW, Hewitt V, Lis A, Morrison C, Robinson A, Todaro E, Wologo E, Wynne S, Alm EW, & Kourtev PS. (2019). Comparative genomics of 16 Microbacterium spp. that tolerate multiple heavy metals and antibiotics. PeerJ 6: e6258.

Meitiniarti VI, Krave AS, Kasmiyati S, & Diyawati RM. (2012). Isolation of Cr(VI) tolerant bacteria from the rhizosphere of Acalypha indica that grows on soil containing textile and tannery waste. Prosiding Seminar Nasional Biologi. Semarang, Indonesia, 30 Oktober 2012. P 86-92.

Meitiniarti VI, Nugroho RA, & Krave AS. (2014). Variety of chromium-reducing bacteria from tannery waste water and rhizosphere of Acalypha indica. Prosiding Seminar Nasional Mikrobiologi. Salatiga, Indonesia, 4 Agustus 2014. pp 59-63.

Miao Q, & Yan J. (2013). Comparison of three ornamental plants for phytoextraction potential of chromium removal from tannery sludge. Master Cycles Waste Manage 15: 98-105.

Murti VM, & Maryani. (2020). Anatomical responses of marigold (Tagetes erecta L.) roots and stems to batik wastewater. Dalam: The 6th International Conference on Biological Science ICBS 2019.

Ouertani R, Ouertani A, Mahjoubi M, Bousselmi Y, Najjari A, Cherif H, Chamkhi A, Mosbah A, Khdhira H, Sghaier H, Chouchane H, Cherif A, & Neifar M. (2020). New plant growth promoting chromium detoxifying Microbacterium species isolated from a tannery wastewater: performance and genomic insights. Frontiers in Bioengineering and Biotechnology 8: 1-14.

Pramono A, Rosariasrtuti RMMA, Ngadiman, & Prijambada ID. (2012). The role of rhizobacteria in the phytoextraction of Chromium heavy metal in corn plant. Jurnal Ecolab 6 (1): 1-60.

Priyanka D, Shalini T, & Navneet VK. (2013). A brief study on marigold (Tagetes Species): a review. International Research Journal Of Pharmacy 4 (1): 43-48.

Sathya V, Mahimairaja S, Bharani A, & Krishnaveni A. (2019). Influence of soil bioamendments on the availability of nickel and phytoextraction capability of marigold from the contaminated soil. International Journal of Plant and Soil Science 31 (5): 1-12.

Shahid M, Shamshad S, Rafiq M, Khalid S, Bibi I, Niazi NK, & Rashid MI. (2017). Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: A review. Chemosphere 178: 513-533.

Sharma A, Kapoor D, Wang J, Shahzad B, Kumar V, Bali AS, Jasrotia S, Zheng B, Yuan H, & Yan D. (2020). Chromium bioaccumulation and its impacts on plants: an overview. Plants 9 (100).

Singh T, & Singh DK. (2019). Rhizospheric Microbacterium sp. P27 showing potential of lindane degradation and plant growth promoting traits. Springer Current Microbiology DOI: 10.1007/s00284-019-01703-x.

Srivastava D, Tiwari M, Dutta P, Singh P, Chawda K, Kumari M, & Chakrabarty D. (2021). Chromium Stress in Plants: Toxicity, Tolerance and Phytoremediation. Journal Sustainability 13: 1-20.

Sun Y, Zhou Q, Xu Y, Wang L, & Liang X. (2011). Phytoremediation for co-contaminated soils of benzo[a]pyrene (B[a]P) and heavy metals using ornamental plant Tagetes patula. Journal of Hazardous Materials 186:2075-2082.

Suryani Y, Cahyanto T, Sudjarwo T, Panjaitan DV, Paujiah E, & Jaenudin M. (2017). Chomium phytoremediation of tannery wastewater using Ceratophyllum demersum. Biosaintifika:Journal of Biology & Biology Education 9 (2): 233-239.

Susana R, & Suswati D. (2013). Bioaccumulation and distribution of Cd in the roots and shoots of 3 types of plants in the Brassicaceae family: its implementation for phytoremediation. Jurnal Manusia dan Lingkungan 20:221-228.

Takahashi H. (2019). Sulfate transport system in plants: functional diversity and molecular mechanisms underlying regulatory coordination. Journal of Experimental Botany 70 (16): 4075–4087.

Thongchai A, Meeinkuirt W, Taeprayoon P, & Pichtel J. (2018). Soil amendments for cadmium phytostabilization by five marigold cultivars. Springer Environmental Science and Pollution Research https://doi.org/10.1007/s11356-019-04233-y.

Veazie P, Cockson P, Henry J, Perkins-Veazie P, & Whipker B. 2020. Characterization of nutrient disorders and impacts on chlorophyll and anthocyanin concentration of Brassica rapa var. Chinensis. Agriculture 10 (10), 461.

Yoon J, Cao X, Zhou Q, & Ma LQ. (2006). Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Science of the Total Environment 368:456-464.

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