The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro
(1) Department of Biology, Faculty of Science and Technology, Airlangga University, Campus C, Mulyorejo, Surabaya, Pos Code 60115, Indonesia
(2) Department of Biology, Faculty of Science and Technology, Airlangga University, Campus C, Mulyorejo, Surabaya, Pos Code 60115, Indonesia
Abstract
Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.
How to Cite
Utami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.
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Bektas, E., Cuce, M., & Sokmen, A. (2013). In vitro germination, protocorm formation, and plantlet development of Orchis coliophora (Orchidaceae), a naturally growing species in Turkey. Turkish Journal of Botany, 37(2), 336-342.
Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics. 11, 1-42.
Dutra, D., Johnson, T. R., Kauth, P. J., Stewart, S. L., Kane, M. E., & Richardson, L. (2008). Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea. Plant Cell, Tissue and Organ Culture, 94(1), 11-21.
Erisen, S., Atalay, L., & Yorgancilar, M. (2011). The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany, 35(5), 521-526.
Fanfani, A., & Rossi, W. (1989). Guide to orchid. Simon & Schuster Inc., New York.
Guo, B., Abbasi, B. H., Zeb, A., Xu, L. L., & Wei, Y. H. (2011). Thidiazuron: A multi-dimensional plant growth regulator. African Journal of Biotechnology, 10(45), 8984-9000.
Hasanah, U., Suwarsi, R. S., & Sumadi. (2014). Utilization of fertilizer leaves, coconut water and banana porridge as component Dendrobium kelemense orchid plantlet growth medium. Biosaintifika: Journal of Biology & Biology Education, 6(2), 161-168.
Jones, D.L. (2006). A complete to native orchids of Australia. Reed New Holland, Sydney.
Kaur, S., & Bhutani K.K. (2012). Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Scientia Horticulturae, 39(1), 47-52.
Long, B., Niemiera, A.X., Cheng, Z.Y., & Long, C.L. (2010). In vitro propagation of four threatened Paphiopedilum species (Orchidaceae). Plant Cell, Tissue and Organ Culture, 101(2), 151-162.
Murashige, T., & Skoog, G. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiology Plant, 15(3), 473-479.
Murdad, R., Latip, M.A., Aziz, Z.A., & Ripin, R. (2010). Effect of carbon source and potato homogenate on in vitro growth and development of Sabah’Endangered orchid: Phalaenopsis gigantea. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18(1), 199-202.
Nambiar, N., Tee, C.S., & Maziah M. (2012). Effect of organic additives and different carbohydrate source on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics Journal, 5(1), 10-18.
Nhut, D. T., Thi, N. N., Khiet, B. L. T., & Luan, V. Q. (2008). Peptone stimulates in vitro shoot and root regeneration of Avocado (Persea americana Mill). Scientia Horticulturae, 115(2), 124-128.
Oluk, E. A., & Orthan, S. (2010). Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra. Afr. Journal of Biotechnology, 8(15), 3506-3510.
Shekarriz, P., Kafi, M., Deilamy, S. D., & Mirmasounii, M. (2014). Coconut water and peptone improve seed germination and protocorm like body formation of hybrid Phalaenopsis. Agriculture Science Development, 3(10), 317-323.
Utami, E.S.W., Purnobasuki, H., Soedarti, T., & Hariyanto, S. (2015). Asymbiotic seed germination and in vitro seedling development of Paphiopedilum liemianum Fowlie, an endangered terrestrial orchid in nothern Sumatra, Indonesia. Journal of Plant Sciences, 10(1), 25-34.
Vogel, I.N., & Maceo, A.F. (2011). Influence of IAA, TDZ and light quality on asymbiotic germination, protocorm formation and plantlet development of Cyrtopodium glutiniferum Raddi., a medicinal orchid. Plant Cell, Tissue and Organ Culture, 104(2), 147-155.
Wu, K., Zeng, S., Lin, D., Teixeira da Silva, J.A., Bu., Z., Zhang, J., & Duan, J. (2014). In vitro propagation and reintroduction of the endangered Renanthera imschootiana Rolfe. PLOS, 9(10), 1-12.
Yong, J. W. H., Ge, L., Fei, Ng Y., & Tan, S. N. (2009). The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules, 14(12), 5144-5164.
Zeng, S., Wu, K., Teixeira da Silva, J.A., Zhang, J., Chen, Z., Xia, N., & Duan, J. (2012). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Scientia Horticulturae, 138, 198-209.
Zeng, S., Wang, J., Wu, K., Teixeira da Silva, J.A., Zhang, J., & Duan, J. (2013). In vitro propagation of Paphiopedilum hangianum Perner & Gruss. Scientia Horticulturae, 151, 147-156.
Zhang, Y., I Lee, Y., Deng, L., & Zhao, S. (2013). Asymbiotic germination of immature seeds and seedling development of Cypripedium macranthos Sw., an endangered lady’s slipper orchid. Scientia Horticulturae, 164, 130-136.
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