Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease

Enni Suwarsi Rahayu(1), Noor Aini Habibah(2),

DOI: https://doi.org/10.15294/biosaintifika.v8i1.5371


(1) Department of Biology, Faculty of Mathematics and Science, Semarang State University, Indonesia
(2) Department of Biology, Faculty of Mathematics and Science, Semarang State University, Indonesia

Abstract

Carica pubescens Lenne & K. Koch, an endemic species in Dieng mountains, must be conserved. The in vitro conservation has been developed, but sub-culture period needs to be extended. This study aimed to obtain a more efficient in vitro conservation protocol of C. pubescens. The research was carried out experimentally by using a completely randomized factorial design with three factors, namely decreasing in storage medium concentration (75% and 50% of MS medium), temperature (4oC and 8°C), and irradiation duration (8 hours/day and 16 hours/day). Shoots were kept in the storage medium for 6, 9 and 12 months, then their viability were tested by growing them in the regeneration medium. Data were analyzed by Analyses of Variance and Least Significant Difference Test. The results showed that medium concentration of 50% of MS, the temperature of 8 °C, and 16 hours/day of irradiation were able to suppress the C. pubescens growth in vitro storage for six months and could maintain its viability in the regeneration medium. Based on these results the medium concentration of 50% of MS, the temperature of 8 °C and 16 hours/day irradiation can be used for in vitro conservation of C. pubescens without sub-culture for six months.

How to Cite

Rahayu, E., & Habibah, N. (2016). Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease. Biosaintifika: Journal of Biology & Biology Education, 8(1), 85-93.

Keywords

Carica pubescens, in vitro storage, irradiation duration, low concentration medium, low temperature

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References

Ahmed, M., Anjum, M.A., Shah, A.H., & Hamid, A. (2010). In Vitro preservation of Pyrus germplasm with minimal growth using different temperature regimes. Pak. J. Bot., 42(3), 1639-1650.

Al-Hussaini, Z. A, Yousif, S. H., & AL-Ajeely, S. A. (2015). The role of sucrose and light duration on in vitro tuberization for two cultivars of potato Solanum tuberosum L. Int. J. Curr. Microbiol. App. Sci. 4(2), 277-283.

Arrigoni-Blank, M.F., Tavares, F.F., Blank, A.F., Santos, M.C., Menezes, T.S.A., & de Santana, A.D.D. (2014). In Vitro Conservation of Sweet Potato Genotypes. The Scientific World Journal, article ID 208506, 7 pages. http://dx.doi.org/10.1155/2014/208506

ASEAN Centre for Biodiversity (2009). ASEAN Centre for Biodiversity: Conserving and managing biodiversity at the regional level. First ASIA-HORCS Joint Symposium. Nagoya, Japan:18-20 July 2009.

Bekheet, S. A. (2000). In vitro preservation of Asparagus officinalis. Biol. Plant, 43(2), 179-183.

Carter, G. A. & Knapp, A. K. (2001). Leaf optical properties in higher plants: linking spectral characteristics to stress and chlorophyll concentration. Am. J. of Botany, 88(4), 677-684.

El-Dawayati, M., Maiada, E. I., Baker, A. H., Gomaa, & Zayed, Z. E. (2013). In vitro conservation of date palm shoot tip explants under minimal growth condition. Egypt. J. Agric. Res. 91(3), 1043-1061.

Elias, M., Wieczorek, G., Rosenne, S., & Tawfik, D. S. (2014). The universality of enzymatic rate- temperature dependency. Trends in Biochem. Sci. 39(1), 1-7.

Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell. Dev. Biol. Plant, 47(1), 5-16.

Georgieva, K. & Lichtenthaler, H. K. (2006). Photosynthetic response of different pea cultivars to low and high temperature treatments. Photosynthetica, 44(4), 569-578.

González, Y. & Cueva, A. (2014). Effects of photoperiod, plant growth regulators and culture media on in vitro growth of seedlings of Cyrtochilum loxense (Lindl.) Kraenzl. An endemic and endangered orchid from Ecuador. Revista Peruana de Biología, 21(2), 189-192.

Kaviani, B. (2010). Conservation of plant genetic resources by cryopreservation. Aust. J. of Crop Sci. 5(6), 778-800.

Lopez-Puc, G. (2013). An effective in vitro slow growth protocol for conservation of the orchid Epidendrum chlorocorymbos Schltr. Tropical and Subtropical Agroecosystems, 16(1), 61 - 68.

Mishra, S. (2011). Effect of temperature, photoperiod and ph on in vitro shoot multiplication of sugarcane. Vegetos, 24(1), 50-53.

Moosikapala, L., & Te-chato, S. (2010). Application of in vitro conservation in Vetiveria zizanioides Nash. J. Agric. Technol, 6(2), 401-407.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15(3), 473-497.

Pérez-Molphe-Balch, E., Pérez-Reyes, M.E., & Rosa-Carrill, D.L. (2012). In vitro conservation of Turbinicarpus (Cactaceae) under slow growth conditions. Haseltonia, 17, 51-57.

Rahayu, E. S., Aini, N. A., & Herlina, L. (2015). In vitro conservation medium of Carica pubescens Lenne & K. Koch by nutrients concentration reduction and osmoregulator addition. Biosaintifika: Journal of Biology & Biology Education, 7(1), 24-30.

Rahayu, E. S., & Sudarsono. (2015). In-vitro selection of drought tolerant peanut embryogenic calli on medium containing polyethylene glycol and regeneration of drought tolerant plants. Emir. J. Food Agric, 27(6), 475-487.

Ramanand & Lal, M. (2004). An efficient protocol for in vitro micropropagation of sugarcane. Sugar Tech, 6 (1&2), 85-87.

Ramage, C. M. & Williams, R. R. (2002). Mineral nutrition and plant morphogenesis. In vitro Cell. Dev. Biol. Plant, 38(2), 116-124.

Reed, B. M. (1999). In vitro storage conditions for mint germplasm. Hortsci. 34(2), 350-352.

Scocchi, A. M., & Mroginski, L. A. (2004). In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions. Phyton (B. Aires), 73, 137-143.

Skalova, I., Viehmannova, I., & Vitamvas, J. (2012). In vitro conservation of Smallanthus sonchifolius under slow-growth conditions. Agric. Tropica et Subtropica, 45(3), 147-150.

Taiz, L. & Zeiger, E. (2010). Plant Physiology. Massachussets: Sinauer Associated Inc.Publishers.

Tokoporo, G.L., Elhassan, A.A., & Ali, M.A. (2013). Effect of nutrient medium concentration and temperature on short- term in vitro conservation of shoot-tip explants of banana. Jonares 1, 37-40.

Tyagi, R.K., Goswami R., Sanayaima R., Singh R., Tandon R., & Agrawal, A. (2009). Micropropagation and slow growth conservation of cardamom (Elettaria cardamomum Maton). In Vitro Cell. Dev. Biol. Plant, 45(6), 721-729.

Uzundzhalieva, K., Ruseva, R., & Kachakova, S. (2014). Ex Situ and In Vitro Conservation of Glicyrrhiza glabra L. - Crop Wild Relative from Fabaceae. Ecologia Balkanica 5, 9-13.

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