AKTIVITAS REACTIVE OXYGEN SPECIES MAKROFAG AKIBAT STIMULASI GEL LIDAH BUAYA PADA INFEKSI Salmonella typhimurium

R. Susanti, A Yuniastuti, RS Iswari

Abstract


Reactive Oxygen Species (ROS) merupakan salah satu lethal chemical yang dapatmembunuh dan mengeliminasi bakteri pada sel fagosit. Lidah Buaya (Aloevera) banyak dipakai sebagai pengobatan tradisional, tetapi belum ada buktiilmiah sampai tingkat seluler apalagi subseluler dalam hal efek imunostimulanpada penyakit infeksi. Tujuan penelitian ini adalah untuk mengetahui aktivitasimunostimulan dari gel lidah buaya yang ditunjukkan oleh aktivitas ROS makrofagsecara in vivo terhadap infeksi bakteri patogen Salmonella typhimurium. Sebanyak24 ekor mencit BABL/c betina umur 8-10 minggu berat 20-30 gram dikelompokkansecara acak menjadi empat kelompok, masing-masing kelompok enam ekor.Kelompok kontrol tidak diberi gel Aloe vera, sementara kelompok P1, P2, dan P3berturut-turut diberi gel Aloe vera 0,5 ml/ekor/hari; 1,0 ml/ekor/hari, dan 1,5ml/ekor/hari. Pemberian gel Aloe vera dilakukan selama sembilan hari. Pada harike-6, mencit diinfeksi bakteri patogen Salmonella typhimurium intraperitoneal105 CFU. Selanjutnya pada hari ke-10 mencit didislokasi dan dibedah, diambilmakrofag dari peritoneum untuk dianalisis produksi ROS-nya. Hasil penelitianmenunjukkan bahwa pemberian gel Aloe vera berpengaruh signi..ikan terhadappeningkatan produksi ROS makrofag mencit BALB/c yang diinfeksi Salmonellatyphimurium. Terdapat perbedaan secara signi..ikan antara kelompok kontroldengan kelompok P1, P2, dan P3, tetapi tidak terdapat perbedaan signi..ikan antarkelompok P1, P2, dan P3. Pemberian gel Aloe vera dosis 0,5 ml/ekor/hari sudahmampu meningkatkan produksi ROS makrofag.

 

Reactive Oxygen Species (ROS) is one of lethal chemicals that can kill and eliminatebacteria in phagocytic cells. Aloe vera is widely used as traditional medicine, but thereis no scienti..ic evidence to prove the effect of immunostimulatory of the Aloe vera gel oninfectious disease in the cellular or subcellular level. This research aims to determinethe immunostimulatory activity of Aloe vera gel showed by ROS macrophage activityin vivo on the infection of bacterial pathogen Salmonella typhimurium. This researchused 24 female mice BABL/c age 8-10 weeks weight 20-30 Gram. They were groupedrandomly in four groups consisting of six mice each. The mice in the control groupwere not given the Aloe vera gel, while group P1 was given the gel of 0,5ml/mice/day,group P2 was 1,0ml/mice/day, and group P3 was 1,5ml/mice/day. The gel was givenin nine days, and in 6th day the mice were infected by bacterial pathogen Salmonellatyphimurium then in 10th day the mice were dissected to take their macrophage fromperitoneum for being analyzed its ROS production. The result showed that there wasa signi..icant difference between control group and P1, P2, and P3 group, but there isno signi..icant difference between P1, P2, and P3 group. In conclusion, the dose of 0,5ml/mice/day was able to increase ROS macrophage production.


Keywords


Aloe vera; ROS; Salmonella typhimurium

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References


Abbas AK & Lichmant AH. 2003. Cellular and Molecular Immunology. Fifth edition. Philadelphia: WB & Saunders

Adrianus W, van der Velden M, Copass MK, Starnbach MN. 2005. Salmonella inhibit T cell proliferation by a direct, contact-dependent immunosuppressive effect. Proc Natl Acad Sci USA 102(49): 17769-17774

Barak Y, Schreber F, Thorne SH, Contag CH, deBeer D & Matin A. 2010. Role of nitric oxide in Salmonella typhimurium-mediated cancer cell killing. BMC Cancer 10: 146-150

Baratawidjaja KG. 2001. Imunologi Dasar. Edisi 5. Jakarta: Balai Penerbit Fakultas Kedokteran. Universitas Indonesia

Bogdan C. 2001. Nitric oxide and the immune response. Nature 2(10): 907-916

Boudreau MD & Beland FA. 2006. An evaluation of the biological and toxicological properties of Aloe barbadensis (Miller), Aloe vera. J Environ Sci Health C. 24: 103-154

Chakravortty D, Hansen-Wester I & Hensel M. 2002. Salmonella pathogenicity island 2 mediated protection of intracellular Salmonella from reactive nitrogen intermediates. J Exp Med. 195: 1155–1166

Diehn M, Cho RW, Lobo NA, Kalisky T, Dorie MJ, Kulp AN, Qian D, Lam JS, Ailles LE, Wong M, Joshua B, Kaplan MJ, Wapnir I, Dirbas FM, Somlo G, Garberoglio C, Paz B, Shen J, Sean K. Lau SK, Quake SR, Brown JM, Weissman IL & Clarke MF. 2009. Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature 458: 780-783

Dutta A, Bandyopadhyay S, Mandal C & Chatterjee M. 2007. Aloe vera leaf exudate induces a caspase-independent cell death in Leishmania donovani promastigotes. J Med Microbiol 56(5): 629-636

Eguchi M & Kikuchi Y. 2010. Binding of Salmonella-specific antibody facilitates specific T cell responses via augmentation of bacterial uptake and induction of apoptosis in macrophages. J Infect Dis 201(1): 62-70

ElSohly MA, Gul W & Murphy TP. 2004. Analysis of the anthraquinones aloe-emodin and aloin by gas chromatography/mass spectrometry. Int Immunopharmacol 4(14): 1739-1744

Esteban A, Zapata JM, Casano L, Martin M & Sabater B. 2000. Peroxidase activity in Aloe barbadensis commercial gel: Probable role in skin protection. Planta Med. 66(8): 724-727

Estevez AG & Jordan J. 2002. Nitric oxide and superoxide, a deadly cocktail. Ann NY Acad Sci. 962: 207-211

Gassem MH. 2001. Typoid Fever, Clinical and Epidemiological Studies in Indonesia. Thesis. Semarang: Diponegoro University Semarang

Goodridge HS, Reyes CN, Becker CA, Katsumoto TR, Ma J, Wolf AJ, Bose N, Chan ASH, Magee AS, Danielson ME, Weiss A, Vasilakos JP & Underhill DM. 2011. Activation of the innate immune receptor Dectin-1 upon formation of a ‘phagocytic synapse’. Nature 472: 471–475

Grover JK, Yadav S & Vats V. 2002. Medical plants of India with anti-diabetic potential. J Ethnopharmacol 81: 81-100

Hamman JH. 2008. Composition and applications of Aloe vera leaf gel. Molecules. 13: 1599-1616.

Haque SS. 2011. Therapeutic effect of nitric oxide and antibiotic against Salmonella typhimurium. J Infect Dis Immun 3(15): 258-261

Haque SS. 2012. Estimation of nitric oxide metabolites and antibiotic against typhoid. 4th International Conference on Bioinformatics and Biomedical Technology. Singapore: IPCBEE

Hawley LB. 2003. Intisari Mikrobiologi dan Penyakit Infeksi. Terjemahan Pendit BU. Jakarta: Hipokrates

Hu Y, Xu J & Hu Q. 2003. Evaluation of antioxidant potential of Aloe vera (Aloe barbadensis Miller) extracts. J Agric Food chem. 51: 7788-7791

Im SA, Oh ST, Song S, Kim MR, Kim DS, Woo SS, Jo YI & Lee CK. 2005. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int Immunopharmacol. 5: 271-279

Kaufman HE. 2001. Immune Response to Intracellular Bacteria. Clinical Immunology Principles and Practice. St. Louis: Mosby

Kresno SB. 2001. Imunologi: Diagnosis dan prosedur laboratorium. Edisi 4. Jakarta. Penerbit Fakultas Kedokteran Universitas Indonesia

Krishnan P. 2006. The scientific study of herbal wound healing therapies: current state of play. Curr Anaesth Crit Care 17: 21-27

Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS & Lee CK. 2001. Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells. Int Immunopharmacol. 1(7): 1275-84

Leung MYK, Liu C, Zhu LF, Hui YZ, Yu B & Fung KP. 2004. Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg. Glycobiol. 14: 501-510

McSorley SJ, Asch S, Costalonga M, Reinhardt RL & Jenkins MK. 2002. Tracking Salmonella-specific CD4 T cells in vivo reveals a local mucosal response to a disseminated. Infect Immun. 16(3): 365-377

Mills PC, Richardson DJ, Hinton JCD & Spiro S. 2005. Detoxification of nitric oxide by the flavorubredoxin of Salmonella enterica serovar Typhimurium. Biochem Soc Trans 33: 198-199

Mohamed EAK. 2011. Antidiabetic, antihypercholestermic and antioxidative effect of Aloe vera gel Extract in Alloxan Induced Diabetic Rats. Aust J Basic Appl Sci. 5(11): 1321-1327

Pugh N, Ross SA, ElSohly MA & Pasco DS. 2001. Characterization of aloeride, a new high-molecular-weight polysaccharide from Aloe vera with potent immunostimulatory activity. J Agric Food Chem. 49:1030-1034

Qiu Z, Jones K, Wylie M, Jia Q & Orndorf S. 2000. Modified Aloe barbadensis polysaccharide with immunorequlatory activity. Planta Med. 66: 152-156

Rishi P, Rampuria A, Tewari R & Koul A. 2008. Phytomodulatory potentials of Aloe vera against Salmonella OmpR-mediated inflammation. Phytother Res 22: 1075–1082

Roitt I, Brostoff J & Male D. 2001. Immunology. Sixth edition. Toronto: Mosby Elsevier Science Limited

Saleem R, Faizi S, Siddiqui BS, Ahmed M, Hussain SA & Qazi A. 2001. Hypotensive effect of chemical constituents from Aloe barbadensis. Planta Med. 67(8): 757-760

Sampedro MC, Artola RL, Murature M, Murature D, Ditamo Y, Roth GA & Kivatinitz S. 2004. Mannan from Aloe saponaria inhibits tumoral cell activation and proliferation. Int. Immunopharmacol. 4: 411-418

Santoso S. 2002. SPSS versi 12. Mengolah data statistik secara profesional. Jakarta: PT Elex Media Komputindo

Saritha V, Anilakumar K R & Khanum F. 2010. Antioxidant and antibacterial activity of Aloe vera gel extracts. IJPBA 1(4): 376-384

Schepetkin IA & Quinn MT. 2006. Botanical polysaccharides: Macrophage immunomo-dulation and therapeutic potential. Int Immunopharmacol 6(3): 317–333

Shirwan H, Mhoyan A, Yolcu ES, Que XY & Ibrahim S. 2003. Chronic cardiac allograft rejection in a rat model disparate for one single class I MHC molecule is associated with indirect recognition by CD4+ T cells. Transplant Immunol. 11: 179–185

Tan BKH & Vanitha J. 2004. Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: A review. Curr Med Chem. 11: 1423-1430

Vahedi G, Taghavi M, Maleki AK & Habibian R. 2011. The effec of Aloe vera extract on humoral dan cellular immune response in rabbit. Afr J Biotechnol. 10(26): 5225-5228

Vazquez-Torres A & Fang FC. 2001. Oxygen-dependent anti Salmonella activity of macrophages. Trends Microbiol. 9: 29–33

Vecchione A, Catchpole B, D’Mello F, Kanellos T & Hamblin A. 2002. Modulating immune responses with dendritic cells: An attainable goal in veterinary medicine. Vet Immunol Immunopathol. 87: 215–221

West AP, Brodsky IE, Rahner C, Woo DK, Erdjument-Bromage H, Tempst P, Walsh MC, Choi Y, Shadel GS & Ghosh S. 2011. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476–480

Xia Q, Boudreau MD, Zhou YT, Yin JJ & Fu PP. 2011. UVB Photoirradiation of Aloe vera– formation of free radicals, singlet oxygen, superoxide, and induction of lipid peroxidation. J Food Drug Anal. 19(4): 396-402

Xia Q, Yin JJ, Fu PP & Boudreau MD. 2007. Photo-irradiation of Aloe vera by UVA-formation of free radicals, singlet oxygen, superoxide, and induction of lipid peroxidation. Toxicol Lett 168(2): 165-75

Xiao BX, Guo JM, Liu DH & Zhang S. 2007. Aloe-emodin induces in vitro G2/M arrest and alkaline phaphatase activation in human oral cancer KB cells. Oral Oncol. 43: 905-910

Xu FG, Liu Y, Zhang ZJ, Song R, DongHJ & Tian Y. 2008. Rapid simultaneous quantification of five active constituents in rat plasma by high-performance liquid chromatography/tandem mass spectrometry after oral administration of Da-Cheng Qi decoction. J Pharm Biomed Anal 47: 586-595

Yagi A & Takeo S. 2003. Anti-inflammatory constituents, aloesin and aloemannan in Aloe species and effects of tanshinon VI in Salvia miltiorrhiza on heart. Yakugaku Zasshi. 123(7): 517-532


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