AKTIVITAS SPERMATOPROTECTIVE EKSTRAK DAUN JAMBU BIJI (Psidium guajava) PADA JUMLAH SPERMATOZOA TIKUS PUTIH TERINDUKSI KADMIUM

W Christijanti, A Marianti

Abstract


Kadmium dapat bersifat meningkatkan aktivitas oksigen reaktif yang memicu munculnya radikal bebas. Zat aktif dalam daun jambu biji bersifat antioksidan yaitu suatu senyawa yang dapat berikatan atau menghambat terbentuknya radikal bebas. Penelitian ini bertujuan untuk mengkaji adanya hubungan antara ekstrak daun jambu biji dengan jumlah spermatozoa tikus terinduksi kadmium. Penelitian eksperimental ini menggunakan 20 ekor tikus yang terinduksi kadmium melalui air minum dengan dosis 100ppm/ekor/hari. Kelompok kontrol mendapatkan ekstrak daun jambu biji 0 mg/ekor sedangkan kelompok I, II dan III berturut turut adalah 50 mg/ekor, 100 mg/ekor dan 150 mg/ekor. Pemberian ekstrak daun jambu biji selama 30 hari. Data jumlah spermatozoa dan kadar kadmium dianalisis dengan uji korelasi pada taraf uji 5%. Pada hari ke 31, testis diambil untuk diukur kadar kadmium dan jumlah spermatozoanya. Hasil penelitian menunjukkan adanya korelasi antara jumlah spermatozoa dan kadar kadmium dengan nilai sig 0,009 <0,05 dan berbanding terbalik sebesar -0,567. Hal ini berarti semakin besar kadar kadium dalam testis maka semakin sedikit jumlah spermatozoa tikus dan semakin kecil kadar kadium dalam testis maka semakin banyak jumlah spermatozoa tikus. Simpulan dari penelitian ini adalah ekstrak daun jambu biji dapat berperan sebagai spermatoprotective tikus yang terpapar Cadmium.

Cadmium can be used to increase the activity of reactive oxygen that triggers the emergence of free radicals. The active substances in guava leaves is an antioxidant compound that can bind or inhibit the formation of free radicals . This study aims to examine the relationship between guava leaf extract with the amount of rat spermatozoa induced by cadmium. This experimental study used 20 mice induced by cadmium through drinking water at dose of 100ppm/mice/day. The control group received guava leaf extract of 0 mg/mice , while group I , II and III received 50 mg , 100 mg and 150 mg in 30 days . The data of the number of spermatozoa and cadmium levels were analyzed by using correlation test in the level of 5 %. The results showed that there was a correlation between the number of spermatozoa and cadmium levels with sig level of 0.009 < 0.05 and inversely at -0.567 . This means that the greater level of cadmium in the mices testicle the less the number of their spermatozoa, therefore the smaller level of cadmium in the mices testicle , the more the number of their spermatozoa . Therefore it can be concluded that guava leaf extract can act as spermatoprotective for mice that were exposed by cadmium.


Keywords


guava leaf extract; spermatozoa; cadmium

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References


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