RESPON PEMBENTUKAN KALUS KORO BENGUK (MUCUNA PRURIENS L.) PADA BERBAGAI KONSENTRASI 2,4-D DAN BAP

R. Ariani, Y. U. Anggraito, E. S. Rahayu

Abstract


Penelitian ini bertujuan untuk menentukan konsentrasi 2,4-dichlorophenoxy acetic acid (2,4-D) dan benzylamino purin (BAP) optimal dalam pembentukan kalus dari eksplan setengah biji koro benguk (Mucuna pruriens L.). Rancangan penelitian yang digunakan ialah rancangan acak lengkap dengan dua faktor, yaitu konsentrasi 2,4-D (0,5 ppm, 1 ppm, 1,5 ppm, 2 ppm) dan BAP (1 ppm, 2 ppm, 3 ppm) untuk induksi kalus. Hasil induksi dipindahkan pada media MS0, kemudian dilanjutkan pada media MS yang ditambah BAP, IBA, GA. Parameter yang diamati adalah waktu terbentuk kalus, persentase eksplan berkalus, berat kalus per eksplan, warna dan tekstur kalus. Pada tahap induksi kalus, BAP 3 mg/l menyebabkan pertumbuhan kalus terberat dibandingkan konsentrasi lainnya. Sementara pada tahap diferensiasi, konsentrasi BAP 3 ppm dan 2,4-D 1 ppm merupakan konsentrasi yang mengakibatkan persentase kalus sehat tertinggi. Perlakuan 2,4-D 1 ppm dan BAP 3 ppm menghasilkan kalus putih transparan, kompak, dan berat kalus tertinggi (0,49 gram). Konsentrasi tersebut merupakan konsentrasi yang disarankan untuk menumbuhkan kalus koro benguk

This research aimed to determine the optimal concentration of 2,4-D and BAP in callus formation from an explant of half seed Mucuna pruriens. The research design was used completely randomized design with two factors: the concentration of 2,4-D (0.5 ppm, 1 ppm, 1.5 ppm, 2 ppm) and BAP (1 ppm, 2 ppm, and 3 ppm) for callus inductions. The induction result is moved to MS0 medium, then continued to MS medium which was added by BAP, IBA, GA. The measured parameters were: callus formation time, the percentage of callus explants, the weight of callus for each explants, color and texture of callus. During induction phase BAP 3 ppm caused the heaviest callus growth than others, meanwhile in differentiation phase BAP 3 ppm and 2,4-D 1 ppm caused the highest percentage of healthy callus. Treatment of 2,4-D 1 ppm and BAP 3 ppm produced white and compact transparent callus with weight 0.49 grams. This concentration was recommended to grow the M. pruriens callus.



Keywords


BAP; 2,4-D; kalus; koro benguk

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