PERKEMBANGAN OVARIUM TIKUS YANG DIPAPAR RADIASI SINAR X

N Nurjanah, P Widiyaningrum

Abstract


Penelitian ini bertujuan untuk mengetahui perkembangan ovarium tikus putih (Rattus norvegicus) galur Wistar yang dipapar radiasi sinar X, meliputi bobot dan gambaran histologisnya. Penelitian menggunakan 20 ekor tikus betina usia 1,5 bulan dengan bobot badan rata-rata 15013 gram. Tikus dikelompokkan ke dalam 4 perlakuan yaitu perlakuan dosis radiasi sinar X sebesar 50, 100 dan 150 mGray serta satu kelompok kontrol. Variabel yang diukur meliputi bobot ovarium utuh dan gambaran histologis ovarium. Radiasi dilakukan dengan cara penyinaran satu kali tembakan pada setiap tikus per hari selama 5 hari. Analisis data bobot ovarium dilakukan dengan dengan anova satu arah dilanjutkan dengan uji LSD pada taraf signifikansi 5%, sedangkan untuk data kualitatif gambaran histologis ovarium dianalisis secara deskriptif. Hasil analisis statistik memperlihatkan bahwa perlakuan dosis radiasi 50, 100 dan 150 mGray berpengaruh nyata terhadap bobot ovarium, yakni cenderung menurun dibanding kelompok kontrol. Pengamatan terhadap gambaran histologis ovarium memperlihatkan kerusakan ovarium mulai terjadi pada dosis 100 dan 150 mGray. Penelitian ini menyimpulkan bahwa paparan radiasi sinar X menyebabkan penurunan bobot ovarium dan pada dosis 100 mGray telah menimbulkan tanda kerusakan pada struktur histologi ovarium tikus putih galur Wistar.

This study aims to determine ovarian development of rats (Rattus norvegicus) after exposure X-ray radiation, especially weights and ovarian histology. The study used 12 female rats of 1.5 months and average body weight of 150 13 grams. Female rats were devided into four groups: control, and variations in dose of 50 mGray (T1), 100 mGray (T2) and 150 mGray (T3). This study used a complete randomized block design. Radiation is done by irradiating one shot on each rat per day for 5 days. Statistical analysis for ovarian weight was performed by one way Anova and LSD Test, while qualitative data of ovarian histological were analyzed descriptively. The results showed that the variations in dose of X-ray radiation significantly (P ? 5 %) of the ovarian weight. LSD test results further demonstrate that treatment of radiation doses 50, 100 and 150 mGray significant effect on ovarian weight, which decreases compared to the control group. This study concluded that exposure to X-ray radiation causes a decrease in ovarian weight and radiation dose of 100 mGray has caused damage to the structure of the ovarian histology Wistar rats.


Keywords


Rattus norvegicus, Ovary development, X-rays, exposure

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References


Aitken JR, Jock KF, Karla JH & Jeff BK. 2011. Apoptosis in the Germ Line. Soc Reprod Fert 141: 139-150.

Alatas Z. 2005. Efek Teratogenik Radiasi Pengion. Pusat Teknologi Keselamatan dan Metrologi Radiasi Batan 6 (3): 133-142.

Behari J. 2010. Biological Responses of Mobile Phone Frequency Exposure. Bioelectromagnetics Laboratory: Jawaharlal Nehru University. Indian J Exp Biol 48: 959-981.

Budiman H, Muslim A & Tongku NS. 2008. Perubahan Histopatologis Ovarium Mencit Akibat Keracunan Etilen Glikol. Jurnal Kedokteran Hewan 2 (2): 159-164.

Fatma LR & Rezk RG. 2004. Evening Primrose Oil Attenuates Certain Radiation Induced Functional and Structural Disorders in Female Rats. Egypt J Hosp Med 16: 119-131.

Fauziyah A & Dwijananti P. 2013. Pengaruh Radiasi Sinar X terhadap Motilitas Sperma pada Tikus Mencit (Mus muculus). Unnes Physic Journal 2 (2): 93-98.

Gaytan F, Morales C, Bellido C, Aguilar E & Sanchez CJE. 1998. Ovarian Follicle Macrophages: Is Follicular Atresia in the Immature Rats a Macrophage-Mediated Event. Biol Reprod (58): 52-59.

Kurniati R. 2009. Keberhasilan Kehamilan dan berat Badan Fetus Mencit (Mus muculus L.) yang Dipapari Radiasi Monitor Komputer. Bioprospek Universitas Mulawarman 6 (2): 23-27.

Lee CJ, Park HH, Do BR, Yoon YD & Jin KK. 2000. Natural and Radiation-Induced Degeneration of Primodial and Primary Follicles in Mouse Ovary. Anim Reprod Sci (59): 109-117.

Nurhayati S, Teja K & Mukh S. 2011. Superoksida Dismutase (SOD): Apa dan Bagaimana Perannya Dalam Radioterapi. Pusat Teknologi Keselamatan dan Metrologi Radiasi Batan. Iptek Ilmiah Populer 13 (2): 67-74.

Rachael UM, Aweda MA, Babatunde EB & Awabajo FO. 2010. Low level microwave exposure decrease the number of male germ cells and effect vital organ of Sprague Dawley rats. American journal. 1(3): 22-32

Rezk RG & Abd EA. 2013. Fucus Vesiculosus Ameliorates Histological and Biochemical Changes in Thyroid Gland and Ovary of Irradiates Rats. Arab J Nucl Sci and Appl 46 (3): 286-296.

Rozaq AI, Munakhir M & Shantiningsih RR. 2013. Perbedaan Jumlah Sel Piknosis Akibat Paparan Radiografi Periapikal Berulang Dua Kali Dibandingkan dengan Radiografi Panoramik pada Pasien RSGM Prof. Soedomo FKG UGM. Bagian Radiologi Dentomaksilofasial. Hlm: 119-123.

Rudi, Pratiwi & Susilo. 2013. Pengukuran Paparan Radiasi Pesawat Sinar-X di Instalasi Radiodiagnostik untuk Proteksi Radiasi. Unnes Physics journal 1 (1): 19- 24.

Suyatno F. 2008. Aplikasi Radiasi Sinar-X di Bidang Kedokteran untuk Menunjang Kesehatan Masyarakat. Seminar Nasional IV SDM Teknologi Nuklir: Yogyakarta. Hlm: 503-510.

Tingen CM, Sarah KBG, Sarah EK, Jason TW, Lonnie S & Teresa KW. 2009. Prepubertal Primodial Follicle Loss in Mice is Not Due to Classical Apoptotic Pathways. Biol Reprod 81: 16-25.

Vahid HJ, Dehghani K, Fatahi E, Nazari M dan Farzam M. 2012. The Effect of Mobile Phone Waves on the Reproduction Physiology in Adult Female Rats. Adv Environ Biol 6 (10): 2735-2741.

Wilmink GJ & Jessica EG. 2011. Current State of Research on Biological Effect of Terahertz Radiation. J Infrared Millim Tech 32: 1074-1122.


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