Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste

Nur Arfa Yanti, Sitti Wirdhana Ahmad, Sri Ambardini, Nurhayani Haji Muhiddin, La Ode Iman Sulaiman


Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.


Acetic Acid Bacteria; Bacterial Cellulose; Pineapple Waste; Sago Liquid Waste

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Abdelhady, H. M., Enas A. H., Sohir S. A. E. & Abdullah, S. M. (2015). Bacterial Cellulose Production as Affected by Bacterial Strains and Some Fermentation Conditions. Nature and Science, 13(3), 30-40.

Cavka, A., Guo, X., Tang, S. J., Winestrand, S., Jnsson, L. J., & Hong, F. (2013). Production of bacterial cellulose and enzyme from waste fiber sludge. Biotechnology for biofuels, 6(1), 25-35.

Chawla, P. R., Bajaj, I. B., Survase, S. A. & Singhal, R. S. (2009). Microbial cellulose: Fermentative production and applications. FFood Technology and Biotechnology, 47(2), 107-124.

Chavez-Pacheco, J. L., Mart?nez-Yee, S., Contreras, M. L., Gomez-Manzo, S., Membrillo-Hernandez, J. & Escamilla, J. E. (2005). Partial bioenergetic characterization of Gluconacetobacter xylinum cells released from cellulose pellicles by a novel methodology. Journal of applied microbiology, 99(5), 1130-1140.

Dewi, P. (2009). Ketahanan Hidup Sel Acetobacter xylinum pada Pengawetan Secara Kering-Beku menggunakan Medium Pembawa. Biosaintifika: Journal of Biology & Biology Education, 1(1), 41-48.

Esa, F., Tasirin, S. M. & Rahman, N. A. (2014). Overview of Bacterial Cellulose Production and Application. Agriculture and Agricultural Science Procedia, 2, 113-119.

Goh, W. N., Rosma A., Kaur, B., Fazilah, A., Karim A. A. & Rajeev B. (2012). Fermentation of black tea broth (Kombucha): I. Effects of sucrose concentration and fermentation time on the yield of microbial cellulose. International Food Research Journal, 19(1), 109-117.

Hemalatha, R. & Anbuselvi, A. (2013). Physicohemical constituents of pineapple pulp and waste.Journal of Chemical and Pharmaceutical Research, 5(2), 240-242

Holt, J. G., Krieg, N. R., Sneath, P. H. A., Stanley, J. T. & Williams, S. T. (1994). Bergeys Manual of Determinative Bacteriology 9th ed., Liplincot, Williams and wilkins, Baltimore.

Hong, F. & Qiu, K. (2008). An alternative carbon source from konjac powder for enhancing production of bacterial cellulose in static cultures by a model strain Acetobacter aceti subsp. xylinus ATCC 23770. Carbohydrate Polymers, 72(3), 545-549.

Keshk, S. M. (2014). Bacterial Cellulose Production and its Industrial Applications. Journal of Bioprocessing & Biotechniques, 4(2), 1-10.

Klawpiyapamornkun, T., Bovonsombut, S. & Bovonsombut, S. (2015). Isolation and Characterization of Acetic acid Bacteria from Fruits and Fermented fruit juices for Vinegar Production. Food and Applied Bioscience Journal, 3(1), 30-38.

Konate, M., Akpa, E. E., Koffi, L. B., Kra, K. A. S., Megnanou, R. M., & Niamke, S. (2014). Isolation of thermotolerant and high acetic acid-producing Acetobacter pasteurianus from Ivorian palm wine. Emirates Journal of Food and Agriculture, 26(9), 773-785

Kongruang, S. (2008). Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products. Applied Biochemistry and Biotechnology, 148(1-3), 245-256.

Mamlouk, D. & Gullo, M. (2013). Acetic Acid Bacteria: Physiology and Carbon Sources Oxidation, Indian journal of microbiology, 53(4), 377-384.

Mohammad, S. M., Rahman, N. A., Khalil, M. S. & Abdullah, S. R. S. (2014). An Overview of Biocellulose Production Using Acetobacter xylinumCulture. Advances in Biological Research, 8(6), 307-313.

Mukadam, T. A., Punjabi, K., Deshpande, S. D., Vaidya, S. P. & Chowdhary, A. S. (2016). Isolation and Characterization of Bacteria and Yeast from Kombucha Tea. International Journal of Current Microbiology and Applied Sciences, 5(6), 32-41

Nadzirah, K. Z., Zainal, S., Noriham, A., Normah, I., Siti Roha, A. M. & Nadya, H. (2013). Physico-chemical properties of pineapple variety N36 harvested and stored at different maturity stages. International Food Research Journal, 20(1), 225-231.

Ochaikul, D., Yamada, Y., Yukphan, P. & Suwanposri, A. (2013). Identification and Biocellulose Production of Gluconacetobacter Strains Isolated from Tropical Fruits in Thailand.Maejo International Journal of Science and Technology, 7(1), 70-82.

Osborne, P. (2010). Advances in microbiological quality control in Managing Wine Quality: Viticulture and Wine Quality A volume in Woodhead Publishing Series in Food Science, Technology and Nutrition, page. 162-188.

Raghunathan, D. (2013). Production of microbial cellulose from the new bacterial strain isolated from temple wash waters. International Journal of Current Microbiology and Applied Sciences, 2(12), 275-290.

Romero-Cortes, T., Robles-Olvera, V., Rodriguez-Jimenes, G. & Ramrez-Lepe, M. (2012). Isolation and characterization of acetic acid bacteria in cocoa fermentation. African Journal of Microbiology Research, 6(2), 339-347.

Sharafi, S. M., Rasooli, I., & Beheshti-Maal, K. (2010). Isolation, characterization and optimization of indigenous acetic acid bacteria and evaluation of their preservation methods. Iranian Journal of Microbiology, 2(1), 41-48

Son, C., Chung, S., Lee, J. & Kim, S. (2002). Isolation and Cultivation Characteristics of Acetobacter xylinum KJ-1 Producing Bacterial Cellulose in Shaking Cultures. Journal of microbiology and biotechnology, 12(5), 722-728.

Suwanposri, A., Yukphan, P., Yamada, Y. & Ochaikul, D. (2013). Identification and biocellulose production of Gluconacetobacter strains isolated from tropical fruits in Thailand. Maejo International Journal of Science and Technology,7(1), 70-82.

Ukwo, S. P., & Ezeama, C. F. (2011). Studies on proliferation of acetic acid bacteria during soursop juice fermentaion. International Journal of Food Safety, 13, 345-350.

Yamada, Y. & Yukphan, P. (2008). Genera and species in acetic acid bacteria. International Journal of Food Microbiology, 125(1), 15-24.



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Biosaintifika: Journal of Biology & Biology Education by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang is licensed under a Creative Commons Attribution 4.0 International Licensep-ISSN (Print) 2085-191X | e-ISSN (Online) 2338-7610