The Effect of Water Content of Medium Containing Oryctes rhinoceros Larvae on Metarhizium anisopliae Pathogenicity

Dyah Rini Indriyanti, Indah Nuraini, Muji Slamet

Abstract


The entomopathogenic fungus, Metarhizium anisopliae (Metschnikoff) Sorokin (Ascomycota: Hypocreales)would effectively infect the target host on the appropriate medium water content. The aim of this study was to analyze the influence of water content of medium on the effectiveness of M. anisopliae fungus infection on O. rhinoceros larvae in the laboratory. Fifty healthy third instar larvae of O. rhinoceros were obtained from field. The M. anisopliae obtained from Estate Crop Protection Board in Salatiga. The conidia density and viability of M. anisopliae were examined before used. The medium for maintaining the larva was the sawdust that had been sterilized. A total of 50 plastic cups were prepared to place 50 larvae (1 larva/cup). Each cup was filled with 100 g medium of sawdust plus 2 g of M. anisopliae which was then stirred until mixed, with different water content: P1 (20%), P2 (40%), P3 (60%), P4 (80% ) and P5 (98%). The result indicated that the water content of the medium affected the effectiveness of M. anisopliae fungus infection on O. rhinoceros larvae. The water content influenced the duration of larval mortality at each treatment. An important finding in this study is that controlling O. rhineceros larvae with M. anisopliae can be done by manipulating the water content of medium. The benefit of this study may be used for the recommendation of O. rhinoceros pest control using M. anisopliae with an effective water media content.


Keywords


Metarhizium anisopliae Pathogenicity; Oryctes rhinoceros mortality; sawdust medium

Full Text:

PDF

References


Abidin, C. M. R. Z., Ahmad, A. H., Salim, H., & Hamid, N. H. (2014). Population Dynamics of Oryctes rhinoceros in Decomposing Oil Palm Trunks in Areas Practising Zero Burning and Partial Burning. Journal of Oil Palm Research, 26(2), 140-145.

Athanassiou, C. G., Kavallieratos, N. G., Rumbos, C. I., Kontodimas, D. C. (2017). Influence of Temperature and Relative Humidity on the Insecticidal Efficacy of Metarhizium anisopliae against Larvae of Ephestia kuehniella (Lepidoptera: Pyralidae) on Wheat. Journal of insect science 17(1), 22

[BPTBUN] Balai Proteksi Tanaman Perkebunan. (2012). Laporan Perbanyakan APH dan Pesnab. Dinas Perkebunan Provinsi Jawa Tengah. Salatiga

Bidochka, M. J., Kamp, A. M. & Decroos, J. N. A. (2000). Insect Pathogenic Fungi: From Genes to Populations. In Fungal Pathology. (Ed) Kronstad J. W. Kluwer Academic Publisher Group, Dordrecht, Netherlands. 171-193p.

Chen, Z. H., Xu, L., Yang, F. L., Ji, G. H., Yang, J., & Wang, J. Y. (2014). Efficacy of Metarhizium anisopliae isolate MAX-2 from Shangri-la, China under desiccation stress. BMC microbiology, 14(1), 4.

Chakravarthy, A. K., Chandrashekharaiah, M., Kandakoor, S. B., & Nagaraj, D. N. (2014). Efficacy of aggregation pheromone in trapping red palm weevil (Rhynchophorus ferrugineus Olivier) and rhinoceros beetle (Oryctes rhinoceros Linn.) from infested coconut palms. Journal of Environmental Biology, 35(3), 479-484.

Gupta, A., & Gopal, M. (2002). Aflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pests. World Journal of Microbiology and Biotechnology, 18(4), 329-335.

Harjaka. (2011). Potensi Jamur Metarhizium anisopliae untuk Pengendalian Uret Perusak Akar Tebu. Fakultas Pertanian UGM. Yogyakarta.

Herlinda, S., Pujiastuti, Y., Pelawi, J., Riyanta, A., Nurnawati, E. & Suwandi. (2005). Patogenisitas isolat-isolat Beauveria bassiana (Bals.) Vuill. Terhadap larva Plutella xylostella (L.) (Lepidoptera: Plutellidae) di rumah kaca. Inovasi, 2(2), 85-92.

Ibrahim, R. A. (2017). Laboratory Evaluation of Entomopathogenic Fungi, Commercial Formulations, against the Rhinoceros Beetle, Oryctes agamemnon arabicus (Coleoptera: Scarabaeidae). Egyptian Journal of Biological Pest Control, 27(1), 49-55.

Indriyanti D. R., Putri, R.I.P., Widiyaningrum, P. & Herlina, L. (2017). Density, Viability conidia and symptoms of Materhizium anisopliae infection on Oryctes rhinoceros larvae. Journal of Physics: Conf.Series 824(2017)012058.

Manurung, E. M., M. C. Tobing, L. Lubis, dan Priwiratama. (2012). Efikasi Beberapa Formulasi Metarhizium anisopliae Terhadap Larva Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) di Insektarium. Jurnal Agroekoteknologi, 1(1), 47-60.

Manjeri, G., Muhamad, R., & Tan, S. G. (2014). Oryctes rhinoceros Beetles, an Oil Palm Pest in Malaysia. Annual Research & Review in Biology, 4(22), 3429-3439.

Mulyono. (2007). Kajian Patogenisitas Cendawan Metarhizium anisopliae Terhadap Hama Oryctes rhinoceros L. Tanaman Kelapa Pada Berbagai Waktu Aplikasi. Thesis. Program Pascasarjana Universitas Sebelas Maret Surakarta.

Moslim, R. & Kamarudin, N. (2014). The use of palm kernel cake in the production of conidia and blastospores of Metarhizium anisopliae var. Major for control of the Oryctes rhinoceros. Journal of Oil Palm Research, 26(2), 133-139.

Permadi, M. A. (2012). Patogenisitas Beberapa Isolat Cendawan Entomopatogen Metarhizium spp Terhadap Kumbang Predator Menochilus sexmaculatus F. (Coleoptera: Coccinelidae). Prodi Agroekoteknologi, BKI Perlindungan Tanaman. Fakultas Pertanian. Universitas Andalas, Kampus Limau Manis, Padang.

Prayogo, Y., Tengkano, W., & Marwoto. (2005). Prospek cendawan entomopatogen metarhizium anisopliae untuk mengendalikan ulat grayak Spodoptera litura pada kedelai. Jurnal litbang pertanian, 24(1), 19.

Susanti, U., Salbiah, D. J., Loah, H. (2013). Uji Beberapa Konsentrasi Metarhizium anisopliae (Metsch) Sorokin Untuk Mengendalikan Hama Kepik Hijau (Nezara viridula L.) Pada Kacang Panjang (Vigna sinensis L.). Jurnal Universitas Riau.

Subhathma, W. G. R., Suwandharathna, N. I., Fernando, L. C. P., & Ahangama, D. (2013). Effect of temperature on lokal and Philippine isolates of Metarhizium anisopliae and their virulence on larvae of Oryctes rhinoceros, a pest of coconut in Sri Lanka. Research Article. Cocos 20: 49-58.

Sodiq, M. (2009). Ketahanan Tanaman Terhadap Hama. Universitas Pembangunan Nasional Veteran. Fakultas Pertanian. Jawa Timur.

Simamora, L. O., Bakti, D. & Oemry, S. (2013). Kajian Epizootik Metarhizium anisopliae pada Larva Tritip (Plutella xylostella L.) (Lepidoptera: Plutellidae) Di Rumah Kaca. Jurnal Online Agroekoteknologi. 1(2), 171.

Tarwotjo, U. & Rahardian, R. (2017). Resistance Inheritance of Plutella xylostella Population to Residual of Emamectin Benzoat. Biosaintifika: Journal of Biology & Biology Education, 9(1), 19-25.




DOI: https://doi.org/10.15294/biosaintifika.v9i2.10262

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.