Exploration of Mango Fruits (Mangifera indica) as α-Glucosidase Inhibitors
(1) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
(2) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
(3) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
(4) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
(5) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
(6) Department of Chemistry, Faculty of Mathematics and Natural Science, Institut Pertanian Bogor
Abstract
Mango fruit (Mangifera indica L.) is the tropical fruit that grows easily in Indonesia with plenty varieties. This study aimed to determine the varieties of mango fruit and the most potent part of mango as antidiabetic agent through α-glucosidase inhibitory activities. Four types of mango fruit (indramayu, manalagi, harum manis, and budiraja) were used in this study. Each part of the mango fruit:peel, flesh, endosperm, and endocarp were extracted by maceration process with three different solvents (n-hexane, ethyl acetate (EtOAc), and ethanol (EtOH)). An ability of all 46 extracts in inhibiting the α-glucosidase at a concentration of 500 ppm were determined. Then 11 extracts with the high inhibition value were determined their IC50 (concentration to inhibit 50% activity) values. EtOAc extract of manalagi, indramayu, and budi raja endosperm had the lowest IC50 value which was not statistically significantly different (at 95%) with EtOAc extract of budi raja peel. The bioautographic Thin Layer Chromatogram showed that the most active band is characterized by white luminescence under UV 366 nm, yellow color under UV 254 and visible light. The band with Rf 0.93 from EtOAc endosperm extract of indramayu and manalagi and Rf 0.73 from EtOAc budi raja peel extract are the most active band which predicted as a flavonoid. The result adds the value of the peel and seed of mango, as well as an alternative in blood sugar control, which is easy to obtain, relatively cheap, and liked by the community.
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Amanatie & Sulistyowati. E. (2015). Structure elucidation of the leaf of Tihoma diversifolia (Hemsl) gray. Jurnal Sains dan Matematika, 23(4), 101-106.
Amir, M. F. (2016). Prediksi formula jamu untuk penyembuhan penyakit diabetes dengan teknik graph mining. Skripsi. Bogor: Institut Pertanian Bogor.
BPOM. (2004). DTCA (Dirrect to Consumer Advertising) dan pengaruhnya. InfoPOM Badan Pengawas Obat dan Makanan Republik Indonesia, 5(3), 1-6
Carrasco-Pozo, C., Tan, K. N., Reyes-Farias, M., De La Jara, N., Ngo, S. T., Garcia-Diaz, D. F., Llanos, P., Cires, M. J., & Borges, K. (2016). The deleterious effect of cholesterol and protection by quercetin on mitochondrial bioenergetics of pancreatic β-cells, glycemic control and inflammation: in vitro and in vivo studies. Redox Biology, 9(2016), 229-243.
Carvalho, R. R., Pellizzon, C. H., Justulin, L., Felisbino, S. L., Vilegas, W., Bruni, F., ... & Hiruma-Lima, C. A. (2009). Effect of mangiferin on the development of periodontal disease: Involvement of lipoxin A 4, anti-chemotaxic action in leukocyte rolling. Chemico-biological Interactions, 179(2), 344-350.
Cheng, A. Y., & Josse, R. G. (2004). Intestinal absorption inhibitors for type 2 diabetes mellitus: prevention and treatment. Drug Discovery Today: Therapeutic Strategies, 1(2), 201-206.
Dar, A., Faizi, S., Naqvi, S., Roome, T., Zikr-ur-Rehman, S., Ali, M., ... & Moin, S. T. (2005). Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biological and Pharmaceutical Bulletin, 28(4), 596-600.
Daud, N. H., Aung, C. S., Hewavitharana, A. K., Wilkinson, A. S., Pierson, J. T., Roberts-Thomson, S. J., ... & Parat, M. O. (2010). Mango extracts and the mango component mangiferin promote endothelial cell migration. Journal of Agricultural and Food Chemistry, 58(8), 5181-5186.
Fauzi, M. R. (2017). Penghambatan α-Glukosidase, Kadar Fenolik dan Flavonoid Total Ekstrak Kulit dan Daging Buah Salak Beberapa Varietas. Skripsi. Bogor: Institut Pertanian Bogor.
Guha, S., Ghosal, S., & Chattopadhyay, U. (1996). Antitumor, immunomodulatory and anti-HIV effect of mangiferin, a naturally occurring glucosylxanthone. Chemotherapy, 42(6), 443-451.
Hiraganahalli, B. D., Chinampudur, V. C., Dethe, S., Mundkinajeddu, D., Pandre, M. K., Balachandran, J., & Agarwal, A. (2012). Hepatoprotective and antioxidant activity of standardized herbal extracts. Pharmacognosy magazine, 8(30), 116-123.
Kumar, B. D., Krishnakumar, K., Jaganathan, S. K., & Mandal, M. (2013). Effect of Mangiferin and Mahanimbine on Glucose Utilization in 3T3-L1 cells. Pharmacognosy magazine, 9(33), 72-75.
Lai, L., Lin, L. C., Lin, J. H., & Tsai, T. H. (2003). Pharmacokinetic study of free mangiferin in rats by microdialysis coupled with microbore high-performance liquid chromatography and tandem mass spectrometry. Journal of Chromatography A, 987(1), 367-374.
Macek, K. (2005). Pharmaceutical Application of Thin Layer and Paper Chromatograph 3rd ed. New York: Elsivier Publishing Company.
Menteri Kesehatan Republik Indonesia. Keputusan Menteri Kesehatan Republik Indonesia. No.: 661/MENKES/SK/VII/1994. Persyaratan obat tradisional.
Morales, M., Zapata, S., Jaimes, T. R., Rosales, S., Alzate, A. E. F., Maldonado, M. E., ... & Rojano, B. I. A. (2017). Mangiferin content, carotenoids, tannins and oxygen radical absorbance capacity (ORAC) values of six mango (Mangifera indica) cultivars from the Colombian Caribbean. Journal of Medicinal Plants Research, 11(7), 144-152.
Muruganandan, S., Srinivasan, K., Gupta, S., Gupta, P. K., & Lal, J. (2005). Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. Journal of ethnopharmacology, 97(3), 497-501.
Núñez Sellés, A. J., Vélez Castro, H. T., Agüero-Agüero, J., González-González, J., Naddeo, F., De Simone, F., & Rastrelli, L. (2002). Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. Journal of Agricultural and Food Chemistry, 50(4), 762-766.
Noratto, G. D., Bertoldi, M. C., Krenek, K., Talcott, S. T., Stringheta, P. C., & Mertens-Talcott, S. U. (2010). Anticarcinogenic effects of polyphenolics from mango (Mangifera indica) varieties. Journal of agricultural and food chemistry, 58(7), 4104-4112.
Nott, P. E., & Roberts, J. C. (1967). The structure of mangiferin. Phytochemistry, 6(5), 741-747.
Ramanathan, J. D., & Seshadri, T. R. (1960). Constitution of mangiferin. Current science, 29, 131-2.
Sarkar, A., Sreenivasan, Y., Ramesh, G. T., & Manna, S. K. (2004). β-D-glucoside suppresses tumor necrosis factor-induced activation of nuclear transcription factor κB but potentiates apoptosis. Journal of Biological Chemistry, 279(32), 33768-33781.
Singh, U. P., Singh, D. P., Singh, M., Maurya, S., Srivastava, J. S., Singh, R. B., & Singh, S. P. (2004). Characterization of phenolic compounds in some Indian mango cultivars. International Journal of Food Sciences and Nutrition, 55(2), 163-169.
Umamahesh, K., Sivudu, S. N., & Reddy, O. V. S. (2016). Evaluation of antioxidant activity, total phenolic and total flavonoids in peels of five cultivar of mango (Mangifera indica) fruit. Journal of Medicinal Plant Studies. 4(2), 200-203.
World Health Organization (2016). Global Report on Diabetes.
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