In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood

Siti Nurhayani, Rita Megia, Ragapadmi Purnamaningsih


A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.  


B. Balcooa; Micropropagation; Wood alternative

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Aggarwal, G., Sharma, C., & Srivastava, D.K. (2012). Thidiazuron: a potent cytokinin for efficient plant regeneration in himalayan poplar (Populus ciliata Wall.) using leaf explants. Annal of Forest Research, 55(2), 179-188.

Astuti, P. (2014). Induksi tunas dan perakaran bambu kuning Bambusa vulgaris secara in vitro. Biogenesis, 2(2), 2302-1616.

Brar, J., Shafi, A., Sood, P., Anand, M., & Sood, A. (2014). In-vitro propagation, biochemical studies and assessment of clonal fidelity through molecular markers in Bambusa balcooa. Journal Tropical Forest Science, 26(1), 115–124.

Fosket, D. E., Morejohn, L. C., & Westerling, K. E., (1977). Control of growth by cytokinin: an examination of tubulin synthesis during cytokinin- induced growth in cultured cells of Paul’s Scarlet Rose. Springer, 193-211.

Gillis, K., Gielis, J., Peeter, H., Dhooghe, E., & Oprins, J. (2007). Somatic embryogenesis from mature Bambusa balcooa Roxburgh as basis for mass production of elite forestry bamboos. Springer, DOI 10.1007/s11240-007-9236-1

George, F. E., & Sherrington, P. D. (1984). Plant propagationby tissue culture. handbook and directory of commercial laboratories. Exogetic Ltd, Britain.

Heddy, S. 1996. Hormon Tumbuhan. PT Raja Grafindo, Jakarta.

Hernandez, A., Castilo, H., Ojeda, D., Aras, A., Lopez, J., & Sanchez, E. (2010). Effect of vermicompost and compost on Lettuce production. Chilean Journal Agriculture Research, 70(4), 583-589.

Irvantia, W., Indriyanto, & Riniarti, M. (2014). Pengaruh jumlah ruas cabang terhadap pertumbuhan setek Bambu Hitam (Gigantochloa atroviolacea). Journal Sylva Lestari, 2 (1), 2339-0913.

Joni, Y. Z., Efendi, D., & Roostika, I. (2015). Induksi perakaran manggis (Garcinia mangostana L.) secara in vitro dan ex vitro. Journal Horticultural, 25(2), 97-105.

Negi, D., & Saxena, S. (2011). Micropropagation of Bambusa balcooa Roxb. through axillary shoot proliferation. International Vitro Cell Devision Biology,. 47, 604–610.

Niranjan, M. H., Sudarshana, M. S., & Girisha, S. T. (2010). In vitro multiple shoot induction from excised shoot tips and nodal segment explants of - Lagerstroemia indica (L) - A medicinal Cum Ornamental Shrub. Journal Biomedicin Science & Research, 2(3), 212-217

Muday, G. K., Rahman, A., & Binder, B. M. (2012). Auxin and ethylene: collaborators or competitors?. Elsevier, doi:10.1016/j.tplants.2012.02.001

Poudyal, B. K., Du, G., Zhang, Y., Liu, J., & Shi, Q. (2008). Studies on browning problem and phenols content on shoots of yali, aikansui and abbe fetel pears for in vitro culture. Front Journal Agriculture China, 2(3), 321–330.

Roostika, I., Sunarlim, N., & Mariska, I. (2008). Micropropagation of Mangosteen. Indonesian Journal Agriculture, 1(1), 28-33.

Sharma, P., Sarma, K. P. (2013). In vitro propagation of Bambusa tulda : an important plant for better environment. Journal Environment Research, 7: 3.

Sinda, K. M. N. K., Kartini, N. L., & Atmaja, I. W. D. (2015). Pengaruh dosis pupuk kascing terhadap hasil tanaman Sawi (Brassica juncea L.), sifat kimia dan biologi pada tanah inceptisol klungkung. Journal Agrotechnology Tropical, 4(3), 2301-6515.

Utami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.

Wattimena, G. A. (1992). Bioteknologi Tanaman. Institut Pertanian Bogor, Bogor

Widjaja, E. A. (2001). Identikit Jenis-Jenis Bambu di Jawa. Pusat Penelitian dan Pengembangan Biologi, Bogor.

Yulizar, D. R., Noli, Z.A., & Idris, M. (2014). Induksi tunas kunyit putih (Curcuma zedoaria roscoe) pada media MS dengan penambahan berbagai konsentrasi BAP dan sukrosa secara in vitro. Journal Bioteknologi, 3(4), 2303-2162.



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