Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia

Afreni Hamidah(1), Evita Anggereini(2), Nurjanah Nurjanah(3),

DOI: https://doi.org/10.15294/biosaintifika.v9i3.11427


(1) Study Program of Biology, Faculty of Teacher Training and Education, Universitas Jambi, Indonesia
(2) Study Program of Biology, Faculty of Teacher Training and Education, Universitas Jambi, Indonesia
(3) Study Program of Biology, Faculty of Teacher Training and Education, Universitas Jambi, Indonesia

Abstract

Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing the number of erythrocytes is 50% papaya leaf juice concentration and 25% papaya leaf juice concentration which gives the best effect in increasing the hemoglobin content. Further, the findings can be used as baseline information for further scientific investigation for using papaya leaf as an alternative medicine in curing anemia disease and analyzing phytochemical, pharmaceutical and other biological activities on other plant or vegetable and to conducted further research by using female mice and pregnant female mice.

Keywords

Anemia; Hematologics disease; Induction Sodium Nitrite; Papaya Leaf Juice

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References

Ambarwati, R. (2012). Effect Of Sodium Nitrite (NaNO2) To Erithrocyte And Hemoglobin Profile In White Rat (Rattus norvegicus). Folia Medica Indonesiana, 8(1), 1-5.

Arisandi, Y., & Andriani, Y. (2008). Khasiat Tanaman Obat Edisi V. Jakarta: Pustaka Buku Murah.

Endreswari, S. (2000). Penelitian Toksisitas Akut Natrium Nitrit Pada Hewan Uji Tikus. Media Penelitian dan Pengembangan Kesehatan, 10(2),12-15.

Gaspersz. (1994). Metode Perancangan Percobaan. Bandung: C.V. Armico.

Hedrich, H. (2014). The Laboratory Mouse. USA: Elsevier Science’s.

Ihwan, I., & Koda, S. H. A. (2017). Antimalarial Herbal Plants in Kupang, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 9(1), 95-104.

Imaga, N. O. A., Gbenle, G. O., Okochi , V. I., Akanbi, S. O., Edeoghon, S. O., Oigbochie, V., Kehinde, M. O., & Bamiro, S. B. (2009). Antisickling property of Carica papaya leaf extract. African Journal of Biochemistry Research, 3(4), 102-106.

Kiswari, R. (2014). Hematologi dan Transfusi. Jakarta: Erlangga.

Kusumawati, D. (2004). Bersahabat dengan Hewan Uji Coba. Yogyakarta: UGM Press.

Mehta, A., & Hoffbrand, V. (2006). Hematology at A Glance. Jakarta: Erlangga.

Milind, P., & Gurditta. (2011). Basketfull Benefits of Papaya. International Research Journal of Pharmacy, 2(7), 6-12.

Ogbe, R. J., Adoga, G. I., & Abu, A. H. (2010). Antianaemic potentials of some plant extracts on phenyl hydrazine-induced anaemia in rabbits. Journal of Medicinal Plants Research, 4(8), 680-684.

Rumimper, E. A., Posangi, J., & Wuisan, J. (2014). Uji Efek Perasan Daun Bayam Merah (Amaranthus tricolor) Terhadap Kadar Hemoglobin Pada Tikus Wistar (Rattus norvegicus). Jurnal e-Biomedik (eBM), 2(2), 1-3.

Sembiring, A., Tanjung, M., & Sabri, E. (2013). Pengaruh Ekstrak Segar Daun Rosela (Hibiscus sabdariffa L.) Terhadap Jumlah Eritrosit dan Kadar Hemoglobin Mencit Jantan (Mus musculus L.) Anemia Strain DDW Melalui Induksi Natrium Nitrit (NaNO2). Saintia Biologi, 1(2), 60-65.

Sianturi, S., Tanjung, M., & Sabri, E. (2013). Pengaruh Buah Terong Belanda (Solanum betaceum Cav.) Terhadap Jumlah Eritrosit dan Kadar Hemoglobin Mencit Jantan (Mus musculus L.) Anemia Strain DDW Melalui Induksi Natrium Nitrit (NaNO2). Saintia Biologi, 1(2), 49-54.

Smith, J. B., & Mangkoewidjojo, S. (1988). Pemeliharaan, Pembiakan, dan Penggunaan Hewan Percobaan di daerah Tropis. Jakarta: Universitas Indonesia.

Suparmi., Sampurna., Nur A. C. S., Alvenia, M. E., Galuh, D. U., Iqrommatul, L. B., & Heavin, R. S. (2016). Anti-anemia Effect of Chlorophyll from Katuk (Sauropus androgynus) Leaves on Female Mice Induced Sodium Nitrite. Pharmacognosy Journal, 8(4), 375-379.

Suudah, E. N., Yusriana, C. S., & Dewi, T. N. (2015). Uji Efektivitas Ketepatan Waktu Pemberian Kombinasi Natrium Tiosulfat dan Natrium Nitrit Sebagai Antidotum Ketoksikan Akut Kalium Sianida Pada Mencit (Mus musculus). Jurnal Permata Indonesia, 6(1), 21-28.

Wolfensohn, S., & Lloyd, M. (1998). Handbook of Laboratory Animal Mnagement and Welfare second edition. USA: Blackwell Science.

Yuningsih. (2007). Keracuanan Nitrat-Nitrit Pada Ternak Ruminansia dan Upaya Pencegahannya. Jurnal Litbang Pertanian, 26(4), 153-159.

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