Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium

Aris Mumpuni, Nuraeni Ekowati, Purnomowati Purnomowati, Endang Sri Purwati


Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.


Balloon-type bubble bioreactor; biomass production; Gynura procumbens; Liquid culture; Temporary immersion system

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Amuneke E. H., Dike K. S., & Ogbulie J. N. (2011). Cultivation of Pleurotus ostreatus: An edible mushroom from agro base waste products. Journal of Microbiology and Biotechnology Research, 1(3), 1-14.

Bonginkhosi, E. D., Diana, M. E., & Masarirambi, M. T. (2012). Growth and Yield Response of Oyster Mushroom (Pleurotus ostreatus) Grown on Different Locally Available Substrates. Current Research Journal of Biological Sciences, 4(5), 623-629.

Carrasco-Gonzalez, J. A., Serna-Sald?var, S. O., & Gutierrez-Uribe, J. A. (2017). Nutritional composition and nutraceutical properties of the Pleurotus fruiting bodies: Potencial use as food ingredient. Journal of Food Composition and Analysis, 58, 69-81.

Chang, S. T., & Miles, P. G. (2004). Edible Mushrooms and Their Cultivation. CRC Press, Inc, Florida: Boca Raton.

Curvetto, N. R., Figlas, D., Devalis, R., Delmastro, S. (2002). Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N-NH4+ and/or Mn (II). Bioresource Technology, 84(2), 171-176.

Deepalakshmi, K & Mirunalini, S. (2014). Pleurotus ostreatus: an oyster mushroom with nutritional and medicinal properties. Journal of Biochemical Technology, 5(2), 718-726

Donini, L. P., Bernardi, E., Minotto, E., & Nascimento, J. S. (2009). Growing Shimeji on elephant grass substrate supplemented with different types of sharps. Scientia Agraria, 1, 67-74

Ekowati N., Kasiamdari, R. S., Pusposendjojo, N., & Soegihardjo, C. J. (2011). Daya Antimikroba Metabolit Bioaktif Jamur Shiitake (Lentinula edodes (Berk.) Pegler) yang Dikultur pada Tiga Jenis Medium Fermentasi. Majalah Obat Tradisional, 16(3), 132-137.

Ekowati, N., Mumpuni, A., & Muljowati, J. S. (2017). Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Carcer Cells. Biosaintifika: Journal of Biology & Biology Education, 9(1), 148-155.

Enyisi, I. S., Umoh, V. J., Whong, C. M. Z., Abdullahi, I. O., & Alabi, O. (2014). Chemical and nutritional value of maize and maize products obtained from selected markets in Kaduna State, Nigeria. African Journal of Food Science and Technology, 5(4), 100-104.

Hoa, H. T., Wang, C. L., & Wang, C. H., (2015). The Effects of Different Substrates on the Growth, Yield, and Nutritional Composition of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(4), 423434.

Iriantinah, C. (2014). Strategi Pengembangan Komoditas Jamur Tiram Putih (Pleurotus florida) Di Kabupaten Nganjuk. Jurnal Manajemen Agribisnis, 14(2), 161-172.

Jeznabadi, E. K., Jafarpo, M., & Ied. S. E. (2016). King oyster mushroom production using various sources of agricultural wastes in Iran. International Journal of Recycling of Organic Waste in Agriculture, 5(1), 17-24.

Lomberh, M. L., Solomko, E. F., Buchalo, A. S., & Kirchhoff, B. (2002). Studies of medicinal mushrooms in submerged cultures. Mushroom Biology and Mushroom Products. Mushroom biology and mushroom products. UAEM, Cuernavaca, 367-377.

Marshall, E., & Nair, N. G. T. (2009). Make money by growing mushrooms. Rural Infrastructure and Agro-Industries Division. Food and Agriculture Organization of the United Nations (FAO). Rome.

Misachi, J. (2017). The Worlds Top Producers of Mushroom and Truffle. Accessed 5 December 2017.

Patil, S. S., Ahmed, S. A., Telang, S. M., & Baig, M. M. V. (2010). The nutritional value of Pleurotus ostreatus (Jacq.:Fr.) Kumm cultivated on different lignocellulosic agrowastes. Innovative Romanian Food Biotechnology, 7, 66-76.

Rashad M. M., Hala, M. A. Abeer, E., Mahmoud, & Nooman, M. U. (2009). Nutritional Analysis and Enzyme Activities of Pleurotus Ostreatus Cultivated on Citrus Limonium and Carica Papaya Wastes. Australian Journal of Basic and Applied Sciences, 3(4), 3352-3360.

Snchez, C. (2010). Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied microbiology and biotechnology, 85(5), 1321-1337.

Sher, H., Al-Yemeni, M., & Khan. K. (2011). Cultivation of the oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) in two different agroecological zones of Pakistan. African Journal of Biotechnology, 10(2), 183-188.

Singh, M. P., Pandey, V. K. Pandey, A. K., Srivastava, A. K., Vishwakarm, N. K., & Singh, V. K. (2008). Production of xylanase by white rot fungi on wheat straw. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 10(4), 859-862.

Teklit, G. A. (2015). Chemical Composition and Nutritional Value of the Most Widely Used Mushrooms Cultivated in Mekelle Tigray Ethiopia. Nutrition and Food Sciences, 5, 408-411

Tirkey, V. J., Simon, S., & Lal, A. A. (2017). Efficacy of different substrates on the growth, yield and nutritional composition of oyster mushroom-Pleurotus florida (Mont.) Singer. Journal of Pharmacognosy and Phytochemistry, 6(4), 1097-1100

Tupamahu, I. P. C. & Budiarso, T. Y. (2017). The effect of oyster mushroom (Pleurotus ostreatus) powder as prebiotic agent on yoghurt quality. American Institute of Physics Conference Proceedings 1844, 030006 (2017)

Vostrovsk, V., & Jablonsk, E. (2011). Geographical analysis of the mushroom Growing possibilities in the developing countries. Agricultura tropica Et Subtropica, 44(4), 229-234

Wakchaure, G. C. (2014). Production and Marketing of Mushrooms: Global and National Scenario. Indian Council of Agricultural Research. Accessed on 5 December 2017.



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