Evaluation of Chromosomal Aberrations and Micronuclei in Medical Workers Chronically Exposed to Low Dose Ionizing Radiation

Yanti Lusiyanti, Iin Kurnia, Viria Agesti Suvifan, Sardini Sardini, Sofiati Purnami, Nastiti Rahajeng

Abstract


Medical workers representing the group is the most consistently are exposed to low doses of ionizing radiation, prolonged low-level ionizing radiation can induce chromosomal aberrations (CAs). This study would evaluate the cytogenetic effect using the CAs based on dicentric, and cytokinesis-blocked micronucleus (CBMN) assay on hospital workers. The exposed group dividedto Interventional and Diagnostic groups then compared to non exposed group. The accumulated absorbed doses calculated for the radiation workers were below 5mSv. Blood samples were obtained from 29 samples of medical workers , and 15 samples of control. The Study showed that the frequency of dicentric chromosomes both in exposed and control were not found. In case of micronuclei, the mean frequencies were observed in exposed group that was (19 6.22) and (16.25 6.04) respectively and the control group was (10.47.79). Frequency MN/1000 cell in the lymphocytes both in the two exposed group was relatively higher compared to control group. However the MN frequencies in all sample group was still in normal range . In this study chronic low radiation dose exposure in the hospital had no significant effect on chromosome aberration nor micronuclei. The benefit of the study is to enrich the potential usefulness of cytogenetic assay providing safety index in medical surveillance programs. The results suggest that education and retraining of staff concerning radiation safety guidelines need to be done to maintain the safety aspects of radiation.


Keywords


Chromosome aberrations; Human lymphocytes; Ionising radiation; Occupational exposure

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References


Agency, International Atomic Energy Agency. (2011). Cytogenetic Dosimetry : Applications in Preparedness for and Response to Radiation Emergencies. Manual Series 247.

Alako, C. & Ero?lu, H. E. (2011). Determining Mitotic Index in Peripheral Lymphocytes of Welders Exposed to Metal Arc Welding Fumes. Turkish Journal of Biology, 35(3), 325-330.

Andreassi, M. Cioppa, A., & Botto, N. (2005). Somatic DNA Damage in Interventional Cardiologists: A Case-Control Study. FASEB Journal, 17(3), 117.

Boffetta, P., van der Hel, O., Norppa, H., Fabianova, E., Fucic, A., Gundy, S., ... & Kelecsenyi, Z. (2006). Chromosomal aberrations and cancer risk: results of a cohort study from Central Europe. American journal of epidemiology, 165(1), 36-43.

Bonassi, S., Znaor, A., Ceppi, M., Lando, C., Chang, W. P., Holland, N., ... & Bigatti, M. P. (2007). An Increased Micronucleus Frequency in Peripheral Blood Lymphocytes Predicts the Risk of Cancer in Humans. Carcinogenesis, 28(3), 62531.

Bouraoui, S., Mougou, S., Drira, A., Tabka, F., Bouali, N., Mrizek, N., ... & Saad, A. (2013). A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers. International journal of occupational medicine and environmental health, 26(1), 144-154.

Cardoso, R. S., Takahashi?Hyodo, S., Peitl, P., Ghilardi?Neto, T., & Sakamoto?Hojo, E. T. (2001). Evaluation of chromosomal aberrations, micronuclei, and sister chromatid exchanges in hospital workers chronically exposed to ionizing radiation. Teratogenesis, carcinogenesis, and mutagenesis, 21(6), 431-439.

Cigarrn, S., Barquinero, J. F., Barrios, L., Ribas, M., Egozcue, J., & Caballin, M. R. (2001). Cytogenetic analysis by fluorescence in situ hybridization (FISH) in hospital workers occupationally exposed to low levels of ionizing radiation. Radiation research, 155(3), 417-423.

Dias, F. L., Antunes, L. M., Rezende, P. A., Carvalho, F. E., Silva, C. M., Matheus, J. M., ... & Balarin, M. A. (2007). Cytogenetic analysis in lymphocytes from workers occupationally exposed to low levels of ionizing radiation. Environmental toxicology and pharmacology, 23(2), 228-233.

Ero?lu, H. E. (2011). The Cytogenetic Effects of Black Tea and Green Tea on Cultured Human Lymphocytes. Brazilian Archives Of Biology And Technology, 54(6), 115965.

Fenech, M. (2007). Cytokinesis-block micronucleus cytome assay. Nature protocols, 2(5), 1084-1104.

Joseph, L. J., Patwardhan, U. N., & Samuel, A. M. (2004). Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 564(1), 83-88.

Kauba, V., Rozgaj, R., & Jazbec, A. (2008). Chromosome aberrations in peripheral blood lymphocytes of Croatian hospital staff occupationally exposed to low levels of ionising radiation. Archives of Industrial Hygiene and Toxicology, 59(4), 251-259.

Kurnia, I., Bintari, S. H., & Khaisuntaha, M. (2012). Tingkat Keganasan Kanker Serviks Pasien Pra-Radiasi Melalui Pemeriksaan AgNORs, MIB-1 dan Cas-3. Biosaintifika: Journal of Biology & Biology Education, 4(2), 53-61.

Lusiyanti, Y., Alatas, Z., Syaifudin, M., & Purnami, S. (2016). Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes. Genome integrity, 7(49), 14.

Lusiyanti, Y., Alatas, Z., Lubis, M., Suvifan, V. A., Ramadhani, D., & Purnami, S. (2013). Dose-response curve of chromosome aberrations in human lymphocytes induced by gamma-rays. Atom Indonesia, 39(3), 124-128.

Maffei, F., Angelini, S., Forti, G. C., Lodi, V., Violante, F. S., Mattioli, S., & Hrelia, P. (2002). Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: influence of smoking status and other factors. Mutagenesis, 17(5), 405-409.

Terzoudi, G. I., & Pantelias, G. E. (2006). Cytogenetic methods for biodosimetry and risk individualisation after exposure to ionising radiation. Radiation protection dosimetry, 122(1-4), 513-520.

Thierens, H., Vral, A., Barbe, M., Meijlaers, M., Baeyens, A., & Ridder, L. D. (2002). Chromosomal radiosensitivity study of temporary nuclear workers and the support of the adaptive response induced by occupational exposure. International journal of radiation biology, 78(12), 1117-1126.

UNSCEAR (United Nations Scientific Committee on the Effects of Atomic Radiation). Sources and Effects of Ionizing Radiation. Report to the General Assembly, (2000); Volume II: Effects. New York: United Nations.

Vral, A., Fenech, M., & Thierens, H. (2011). The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. Mutagenesis, 26(1), 11-17.

Zakeri, F., & Hirobe, T. (2010). A cytogenetic approach to the effects of low levels of ionizing radiations on occupationally exposed individuals. European journal of radiology, 73(1), 191-195.




DOI: https://doi.org/10.15294/biosaintifika.v9i3.12382

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