Effect of Manure and Inorganic Fertilizers on Vegetative, Generative Characteristics, Nutrient, and Secondary Metabolite Contents of Mungbean

Sutrisno Sutrisno, Eriyanto Yusnawan

Abstract


A field experiment was conducted in the upland of Muneng Probolinggo during early dry season (April – June 2015) to study the effect of manure and inorganic fertilizers on vegetative, generative growth, nutrient, and secondary metabolite contents of mungbean (Vigna radiata L.). The treatments consisted of (1) no fertilizer, (2) 10.4, 18, 60 kg of N, P, K ha-1, (3) 22.5: 22.5: 22.5 kg of NPK ha-1, (4) 5000 kg ha-1 manure, and (5) 11.25:11.25:11.25 kg of NPK ha-1 + 2500 kg ha-1 manure. These treatments were arranged in a randomized completely block design with three replications. Application of manure, inorganic fertilizer, and its combination significantly stimulated several vegetative characters especially number of nodes, number of clusters, fresh weight of biomass, and number of nodules. At the generative characters, application of manure and inorganic fertilizers also significantly increased pod dry weight and grain dry weight. Among all treatments, NPK inorganic fertilizer (T2) gave the highest vegetative and generative growth which was shown on biomass fresh weight, pod dry weight and grain dry weight per plant. NPK fertilizer and manure applications increased total flavonoid and phenolic contents as well as antioxidant activity. This combination treatment therefore, could be suggested in mungbean cultivation to increase seed quality.


Keywords


Fertilizers; Growth performance; Mungbean; Nutrient; Secondary metabolite

Full Text:

PDF

References


AOAC. (2005). Microchemical determination of nitrogen using microKjeldhal method (12.1.07). Official Methods of Analysis of AOAC International. Vol. I. Agricultural Chemicals, Contaminants, Drugs. AOAC International. Gaithersburs, Maryland, USA.

Balai Penelitian Tanah. (2009). Petunjuk Teknis Analisis Kimia Tanah, Tanaman , Air dan Pupuk. Balai Penelitian tanah. 234 pp.

Bandyopadhyay, K. K., Misra, A. K., Ghosh, P. K., & Hati, K. M. (2010). Effect of integrated use of farmyard manure and chemical fertilizers on soil physical properties and productivity of soybean. Soil and Tillage Research, 110(1), 115–125.

Bavec, M., Turinek, M., Grobelnik-Mlakar, S., Slatnar, A., & Bavec, F. (2010). Influence of industrial and alternative farming systems on contents of sugars, organic acids, total phenolic content, and the antioxidant activity of red beet (Beta vulgaris L. ssp. vulgaris Rote Kugel). Journal of Agricultural and Food Chemistry, 58(22), 11825–11831.

Chaudhary, S., Dheri, G. S., & Brar, B. S. (2017). Long-term effects of NPK fertilizers and organic manures on carbon stabilization and management index under rice-wheat cropping system. Soil and Tillage Research, 166, 59–66.

Eo, J., & Park, K.-C. (2016). Long-term effects of imbalanced fertilization on the composition and diversity of soil bacterial community. Agriculture, Ecosystems & Environment, 231, 176–182.

Fujikake, H., Yashima, H., Sato, T., Ohtake, N., Sueyoshi, K., & Ohyama, T. (2002). Rapid and reversible nitrate inhibition of nodule growth and n2 fixation activity in soybean (glycine max (l.) merr.). Soil Science and Plant Nutrition, 48(2), 211–217.

Heimler, D., Vignolini, P., Dini, M. G., & Romani, A. (2005). Rapid Tests to Assess the Antioxidant Activity of Phaseolus vulgaris L. Dry Beans. Journal of Agricultural and Food Chemistry, 53(8), 3053–3056.

Ibrahim, M. H., Jaafar, H. Z. E., Karimi, E., & Ghasemzadeh, A. (2013). Impact of organic and inorganic fertilizers application on the phytochemical and antioxidant activity of Kacip Fatimah (Labisia pumila Benth). Molecules, 18(9), 10973–10988.

Jannoura, R., Joergensen, R. G., & Bruns, C. (2014). Organic fertilizer effects on growth, crop yield, and soil microbial biomass indices in sole and intercropped peas and oats under organic farming conditions. European Journal of Agronomy, 52, 259–270.

Jeng, T. L., Shih, Y. J., Wu, M. T., & Sung, J. M. (2010). Comparisons of flavonoids and anti-oxidative activities in seed coat, embryonic axis and cotyledon of black soybeans. Food Chemistry, 123(4), 1112–1116.

Juliano, B. O. (1979). A simplified assumy for milled rice amylose. Cereal Sci. Today, 16, 334-340.

Kuntyastuti, H. & Lestari, S. A. D. (2016). Effect of Fertilizer Dosage and Plant Population onMungbean Growth and Yield Performance at Upland with Dry Climates. Jurnal Penelitian Pertanian Tanaman Pangan, 35(3), 239-250.

Lin, L., HE, Y., & Chen, J. (2016). The influence of soil drying- and tillage-induced penetration resistance on maize root growth in a clayey soil. Journal of Integrative Agriculture, 15(5), 1112–1120.

Malik, M. A., Iqbal, R. M., & Ayyoub, M. (1988). Seed Yield and Qulity of Mungbean (Vigna radiata L) as Influenced by NPK Application. Pakistan Journal Agriculture Science, 25(2), 97–100.

Mandal, A., Patra, A. K., Singh, D., Swarup, A., & Masto, R. E. (2007). Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stages. Bioresource Technology, 98(18), 3585–3592.

Mandal, K. G., Hati, K. M., & Misra, A. K. (2009). Biomass yield and energy analysis of soybean production in relation to fertilizer-NPK and organic manure. Biomass and Bioenergy, 33(12), 1670–1679.

Manna, M. C., Swarup, A., Wanjari, R. H., Mishra, B., & Shahi, D. K. (2007). Long-term fertilization, manure and liming effects on soil organic matter and crop yields. Soil and Tillage Research, 94(2), 397–409.

Montalba, R., Arriagada, C., Alvear, M., & Zúñiga, G. E. (2010). Effects of conventional and organic nitrogen fertilizers on soil microbial activity, mycorrhizal colonization, leaf antioxidant content, and Fusarium wilt in highbush blueberry (Vaccinium corymbosum L.). Scientia Horticulturae, 125(4), 775–778.

Oktavia S. Padmini, Tohari, D. P., & Syukur, A. (2008). Peran residu legum-padi dan campuran pupuk organik - anorganik terhadap hasil padi dan kandungan amilosa – amilopektin beras. Jurnal ilmu pertanian, 31(2), 178–183.

Parvej, M. R., Slaton, N. A., Purcell, L. C., & Roberts, T. L. (2015). Potassium Fertility Effects Yield Components and Seed Potassium Concentration of Determinate and Indeterminate Soybean. Agronomy Journal, 107(3), 943–950.

Parvej, M. R., Slaton, N. A., Purcell, L. C., & Roberts, T. L. (2016). Soybean Yield Components and Seed Potassium Concentration Responses among Nodes to Potassium Fertility. Agronomy Journal, 108(2), 854–863.

Qin, H., Lu, K., Strong, P. J., Xu, Q., Wu, Q., Xu, Z., Wang, H. (2015). Long-term fertilizer application effects on the soil, root arbuscular mycorrhizal fungi and community composition in rotation agriculture. Applied Soil Ecology, 89, 35–43.

Salama, Z. A., El Baz, F. K., Gaafar, A. A., & Zaki, M. F. (2015). Antioxidant activities of phenolics, flavonoids and vitamin C in two cultivars of fennel (Foeniculum vulgare Mill.) in responses to organic and bio-organic fertilizers. Journal of the Saudi Society of Agricultural Sciences, 14(1), 91–99.

Sharma, G. D., Thakur, R., Raj, S. O. M., Kauraw, D. L., & Kulhare, P. S. (2013). Impact of Integrated Nutrient Management on Yield , Nutrient Uptake , Protein Content of Wheat ( Triticum Astivam ) and Soil Fertility in a Typic Haplustert. The Bioscan, 8(4), 1159–1164.

Shi, Z., Yao, Y., Zhu, Y., & Ren, G. (2016). Nutritional composition and antioxidant activity of twenty mung bean cultivars in China. The Crop Journal, 4(5), 398–406.

Singh, M., Yadav, S. K., Kumar, N., Ojha, M. D., & Kumar, V. (2017). Effect of organic manures and NPK on nodulation, microbial biomass carbon and yield of soybean. Environment and Ecology, 35(3), 1605–1609.

Sudarmadji, S., B. Haryono, and Suhardi. 1997. Analytical Procedures for Food and Agricultural Materials. Liberty. Yogyakarta.

Sugiyama, A., & Yazaki, K. (2014). Flavonoids in plant rhizospheres: Secretion, fate and their effects on biological communication. Plant Biotechnology, 31(5), 431–443.

Supanjani, S., Lee, K. D., Almaraz, J. J., Zhou, X., & Smith, D. L. (2006). Effect of organic N source on bacterial growth, lipo-chitooligosaccharide production, and early soybean nodulation by Bradyrhizobium japonicum. Canadian Journal of Microbiology, 52(3), 227–236.

Tadesse, T., Dechassa, N., Bayu, W., & Gebeyehu, S. (2013). Effects of Farmyard Manure and Inorganic Fertilizer Application on Soil Physico-Chemical Properties and Nutrient Balance in Rain-Fed Lowland Rice Ecosystem. American Journal of Plant Sciences, 4(February), 309–316.

Taie, H. a. a., Radwan, S., & El-Mergawi, R. (2008). Isoflavonoids, Flavonoids, Phenolic Acids Profiles and Antioxidant Activity of Soybean Seeds as Affected by Organic and Bioorganic Fertilization. American-Eurasian Journal of Agricultural & Environmental Science, 4(2), 207–213.

Wei, M., Hu, G., Wang, H., Bai, E., Lou, Y., Zhang, A., & Zhuge, Y. (2017). 35 years of manure and chemical fertilizer application alters soil microbial community composition in a Fluvo-aquic soil in Northern China. European Journal of Soil Biology, 82, 27–34.

Wei, W., Yan, Y., Cao, J., Christie, P., Zhang, F., & Fan, M. (2016). Effects of combined application of organic amendments and fertilizers on crop yield and soil organic matter: An integrated analysis of long-term experiments. Agriculture, Ecosystems and Environment, 225, 86–92.

Wongekalak, L. ongdao, Sakulsom, P., Jirasripongpun, K., & Hongsprabhas, P. (2011). Potential use of antioxidative mungbean protein hydrolysate as an anticancer asiatic acid carrier. Food Research International, 44(3), 812–817.

Xu, B., & Chang, S. K. C. (2008a). Antioxidant Capacity of Seed Coat, Dehulled Bean, and Whole Black Soybeans in Relation to Their Distributions of Total Phenolics, Phenolic Acids, Anthocyanins, and Isoflavones. Journal of Agricultural and Food Chemistry, 56(18), 8365–8373.

Xu, B., & Chang, S. K. C. (2008b). Total Phenolics, Phenolic Acids, Isoflavones, and Anthocyanins and Antioxidant Properties of Yellow and Black Soybeans As Affected by Thermal Processing. Journal of Agricultural and Food Chemistry, 56(16), 7165–7175.

Xu, B. J., & Chang, S. K. C. (2007). A Comparative Study on Phenolic Profiles and Antioxidant Activities of Legumes as Affected by Extraction Solvents. Journal of Food Science, 72(2), S159–S166.

Zhong, W., Gu, T., Wang, W., Zhang, B., Lin, X., Huang, Q., & Shen, W. (2010). The effects of mineral fertilizer and organic manure on soil microbial community and diversity. Plant and Soil, 326(1–2), 511–522.




DOI: https://doi.org/10.15294/biosaintifika.v10i1.12716

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.