Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria

Dini Ryandini, Hendro Pramono, Sukanto Sukanto

Abstract


Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria


Keywords


Antibacterial activity; Multi-Drugs Resistant bacteria; Streptomyces sp. SAE4034; 16SrRNA analysis

Full Text:

PDF

References


Ashokkumar, L., R. Balagurunathan, P. Palanivel, & D.Jegadeeshkumar. (2012). Studies on Antimicrobial Activity of Actinomycetes Against MDR Wound Bacterial Isolates. International Journal of Applied Biology and Pharmaceutical Technology, 3(4), 118-123.

Ayari, A., H. Morakchi & K.G. Djamila. (2016). Isolation of Antifungal Activity of Novel Marine Actinomycete, Streptomyces sp. AA13 Isolated from Sediments of Lake Ougeria (Algeria) Against Candida albicans. African Journal of Microbiology Reseacrh, 10(6), 156-171.

Al-Zahrani, S.H. (2007). Studies on the Antimicrobial Activity of Streptomyces sp. Isolated from Jazan. JKAU,19, 127-138 .

Bhat, S.A., R. Nazir, T.A. Malik, & F.A. Shah. (2013). Secondary metabolites of actinomycetes as potential source of antibiotics. Stem Cell, 4(2), 41-46.

Cappuccino, J.G. and N. Sherman. (1987). Microbiology : A Laboratory Manual. The Benjamin/Cummings Publishing Co., Inc.

Das, S., P. S. Lyla & S.A. Khan. (2006). Marine Microbial Diversity and Ecology: Importance and Future Perspectives. Current Science, 90(10), 1325-1335.

El-Naggar, N.E., A.A. Sherief, & S.S. Hamza. (2011). Streptomyces aegyptia NEAE 102, A Novel Cellulolytic Streptomycete Isolated from Soil in Egypt. African Journal of Microbiology Research, 5(29), 5308-5315.

Hamza, A.A., H.A. Ali, B.R. Clark, C.D. Murphy, & E.A. Elobaid. (2013). Isolation and Characterisation of Actinomycin D Producing Streptomyces spp. from Sudanese Soil. African Journal of Biotechnology, 12(19), 2624-2632.

Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, & S.T. Williams. (2000). Bergey’s Manualof Determinative Bacteriology. 9th ed. Lippincott Williams & Wilkins. Philadelphia, USA.

Hu, H., H. Lin, Q. Xie, L. Li, X. Xie, & K. Hong. (2012). Streptomyces qinglanensis sp. nov., Isolated from Mangrove Sediment. International Journal of Systematic and Evolutionary Microbiology, 6, 596–600.

Janaki, T. 2016. Biocontrol of Rhizoctonia Solani by Streptomyces cacaoi Subsp cacaoi.M20. World Journal of Pharmacy and Pharmaceutical Sciences, 5(8), 1516-1527.

Kekuda, T.R.P., K.S. Shobba, & R. Onkarappa. (2010). Fascinating Diversity and Potent Biological Activities of Actinomycete Metabolites. Journal of Pharmacy Research, 3(2), 250-256.

Khanna, M., R. Solanki, & R. Lal. (2011). Selective Isolation of Rare Actinomycetes Producing Novel Antimicrobial Compounds. International Journal of Advanced Biotechnology and Research, 2(3), 357-375

Mahesh.M. and M. Nath. (2013). Effect of Corbon and Nitrogen Source for the Production of Tetracycline Analysis by Using HPLC International Journal of Advanced Biotechnology and Research, 4(2), 218-223

Malviya, N., A.K. Yadav, M.S. Yandigeri, & D.K. Arora. (2011). Diversity of Culturable Streptomycetes from Wheat Cropping System of Fertile Regions of Indo-Gangetic Plains, India. World J Microbiol Biotechnol, 27:1593–1602

Miyadoh, S. 1997. Atlas of Actinomycetes. Asakura Publishing Co., Ltd., Japan.

Nurkanto, A. and A. Agusta. (2015). Identifikasi Molekuler dan Karakterisasi Morfo-Fisiologi Actinomycetes Penghasil Senyawa Antimikroba. Jurnal Biologi Indonesia, 11(2), 195-203.

Okami, Y. & K. Hotta. 1988. Search and Discovery of New Antibiotics. M. Goodfellow, S.T. Williams, M. Mordaski (Eds.), Actinomycetes in Biotechnology, Academic Press, London (1988), 33-67

Pandey, N, A. Shukla, S.K. Majumdar. (2005). Utilization of Carbon and Nitrogen Sources by Streptomyces kanamyceticus M 27 for the Production of an Anti Bacterial Antibiotic. African Journal Of Biotechnology, 4(9), 909-910.

Radjasa, O.K. (2013). Bioprospecting of Marine Microbial Symbionts: Exploitation of Underexplored Marine Microorganisms. Chapter 21. Marine Microbiology: Bioactive Compounds and Biotechnological Applicationss. Wiley-VCH. Verlag GmbH & Co. KGaA.

Sharma, M. (2014). Actinomycetes: Source, Identification, and Their Applications. Int. J. Curr. Microbiol. App.Sci, 3(2), 801-832.

da Silva, M.A.C., A. Cavalett, A. Spinner, D.C. Rosa, R.B. Jasper , M.C. Quecine , M.L. Bonatelli , A.P. Kleiner, G.Corção & A.O.S. Lima. (2013). Phylogenetic Identification of Marine Bacteria Isolated from Deep-Sea Sediments of the Eastern South Atlantic Ocean. SpringerPlus, (2):127

Sipriyadi, S., Y. Lestari, A. Wahyudi, A. Meryandini & M.T. (2016). Exploration Potential CIFOR Forest Actinomycetes Origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology & Biology Education, 8(1), 96-104.

Song J., S.C. Lee, J.W. Kang, , H.J. Baek & J.W. Suh. (2004). Phylogenetic Analysis of Streptomyces spp. Isolated from Potato Scab Lesions in Korea on the Basis 16S rRNA Gene and 16S-23S rDNA Internally Transcribed Spacer Sequences. International Journal of Systematic and Evolutionary Microbiology, 54, 203-209




DOI: https://doi.org/10.15294/biosaintifika.v10i1.12896

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.