Antibacterial and Antibiofilm Activity of Daemonorops draco Resin
(1) Department of Chemistry, Institut Pertanian Bogor
(2) Department of Chemistry, Institut Pertanian Bogor, Indonesia
(3) Department of Chemistry and Tropical Biopharmaca Research Center, Institut Pertanian Bogor
Abstract
Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that  D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.
Keywords
Full Text:
PDFReferences
Andhika, R. R., Hariyadi, B., & Saudagar, F. (2015). Etnobotany of Sap Producing Plants by Suku Anak Dalam in the National Park Bukit Duabelas Sarolangun, Jambi. J Ilmu Pertanian Indonesia, 20(1), 33–38.
Archer, N. K., Mazaitis, M. J., Costerton, J. W., Leid, J. G., Powers, M. E., & Shirtliff, M. E. (2011). Staphylococcus aureus biofilms. Virulence, 2(5), 445–459.
Arnone, A., Nasini, G., De Pava, O. V., & Merlini, L. (1997). Constituents of Dragon’s blood. 5. Dracoflavans B1, B2, C1, C2, D1, and D2, new A-type deoxyproanthocyanidins. J Nat Prod, (60), 971–975.
Batubara, I., Mitsunaga, T., & Ohashi, H. (2009). Screening antiacne potency of Indonesian medicinal plants: Antibacterial, lipase inhibition, and antioxidant activities. J Wood Sci, 55(3), 230–235.
Batubara, I., Wahyuni, W. T., & Susanta, M. (2016). Antibacterial activity of zingiberaceae leaves Essential oils against Streptococcus mutans and teeth-biofilm degradation. Int J Pharma Bio Sci, 7(4), 111–116.
Bazargani, M. M., & Rohloff, J. (2016). Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food Control, 61, 156–64.
Gupta, D., & Gupta, R. K. (2011). Bioprotective properties of Dragon’s blood resin: In vitro evaluation of antioxidant activity and antimicrobial activity. BMC Complement Altern Med, 11(13), 11-13.
Hao, Q., Saito, Y., Matsuo, Y., Li, H. Z., & Takashi, T. (2015). Three new flavans in dragon’s blood from Daemonorops draco. Nat Prod Res, 29(15), 1419–25.
Kuo P. C., Hung, H. Y., Hwang, T. L., Du, W. K., Ku, H. C., & Lee, E. J. (2017). Anti-inflammatory Flavan-3-ol-dihydroretrochalcones from Daemonorops draco. J Nat Prod, 80(4), 783–9.
Millar, B. C., Prendergast, B. D., & Moore, J. E. (2008). Community-associated MRSA (CA-MRSA): An emerging pathogen in infective endocarditis. J Antimicrob Chemother, 61(1), 1–7.
Nakashima, K. I., Abe, N., Kamiya, F., Ito, T., Oyama, M., & Iinuma, M. (2009). Novel flavonoids in dragon’s blood of Daemonorops draco. Helvetica Chim Acta, 92(10), 1999–2008.
O’Toole, G., Kaplan, H. B., & Kolter, R. (2000). Biofilm formation as microbial development. Annu Rev Microbiol, 54, 49–79.
Phuong, N. T. M., Van Quang, N., Mai, T. T., Anh, N. V., Kuhakarn, C., & Reutrakul, V. (2017). Antibiofilm activity of α-mangostin extracted from Garcinia mangostana L. against Staphylococcus aureus. Asian Pac J Trop Med, 10(12), 1154-1160.
Pratiwi, S. U. T., Lagendijk, E. L., Hertiani, T., De Weert, S., Cornellius, A. M., & Van Den Hondel, J. J. (2015). Antimicrobial effects of indonesian medicinal plants extracts on planktonic and biofilm growth of Pseudomonas aeruginosa and Staphylococcus Aureus. J Horticulture, 2(1), 119–132.
Rao, G., Gerhart, M., Lee, R., Mitscher, L., & Drake, S. (1982). Antimicrobial agents from higher plants. dragon’s blood resin. J Nat Prod, 45(5), 646–8.
Rossi, D., Guerrini, A., Maietti, S., Bruni, R., Paganetto, G., & Poli, F. (2011). Chemical fingerprinting and bioactivity of Amazonian Ecuador Croton lechleri Müll. Arg. (Euphorbiaceae) stem bark essential oil: A new functional food ingredient. Food Chem, 126(3), 837–848.
Sanchez, E., Morales, C. R., Castillo, S., Leos-Rivas, C., Garcia-Becerra, & Martinez, D. M. O. (2016). Antibacterial and antibiofilm activity of methanolic plant extracts against nosocomial microorganisms. Evid based complement alternat med. PMCID: PMC4939345
Stewart, P. S. (2002). Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol, 292(2), 107–13.
Teanpaisan, R., Kawsud, P., Pahumunto, N., & Puripattanavong. (2017). Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms. J. Trad complement med, 7 (2), 172-177.
Termentzi, A., Fokialakis, N., & Skaltsounis, A. L. (2011). Natural resins and bioactive natural products thereof as potential anitimicrobial agents. Curr Pharm Des, 17(13), 1267–90.
Tillah, M., Batubara, I., & Sari, R. K. (2017). Antimicrobial and antioxidant activities of resins and essential oil from pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia). Biosaintifika J Biol Biol Educ, 9(1), 134-139.
Wahyuni, W. T., Batubara, I., & Tambunan, D. Y. (2017). Antibacterial and teeth biofilm degradation activity of Curcuma aeruginosa essential oil. J Biol Sci, 17, 84–90.
Yu, J. H., Zheng, G. B, Liu, C. Y., Zhang, L. Y., Gao, H. M., & Zhang, Y. H. (2013). Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways. Int J Med Sci, 10(9), 1149–56.
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.