Histology Structure of Lymph and Uterus of White Rat (Rattus norvegicus) Given with Nanas Bongsai (Ananas comosus var. microstachys L.)

Fitmawati Fitmawati(1), Agus Saputra(2), Yohanes Yohanes(3), Hilwan Yuda Teruna(4), Dimas Pramita Nugraha(5), Yulis Hamidi(6),


(1) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau Kampus
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau Kampus
(3) Public Health Official, Riau Province
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau
(5) Department of Medical, Faculty of Mendical, Universitas Riau
(6) Department of Medical, Faculty of Mendical, Universitas Riau

Abstract

Nanas Bongsai (Ananas comosus var. microstachys L.) is an amazing herb which is used in traditional medicine by local people of Muara Lembu district as a potion to reduce pain while menstruation period for a women. The present work was designed to investigate its probable side effects on the histopathologic changes in limph and uterus tissues after treated with A. comosus var. microstachys L extract in female white rats. This is an experimental research consisted of five treatments and three repetitions. Treatment composed of two controls (P0 and P+) and given Nanas Bongsai extract with three different dosages. Histology result of lymph show that all treatments given with Nanas Bongsai with three different dosages have different diameter average of white pulp and there is no significant changes with normal control. While observation towards histology structure of endometrium thickness with dosage P1, dosage P2, dosage P3 is 206.333±33.486; 215.667±33.486; 197.667±60.871 respectively. The most thin endometrium layer found in treatment with dosage P3 compared to control P0. From this study showed that there is no toxic effect on uterus and lymph function of of Nanas Bonsai (A. comosus var. microstachys L.) at different doses, so the plant is secure for consumption by the community.

Keywords

Nanas Bongsai (Ananas comosus var. microstachys L.); White rat (Rattus norvegicus); Lymph; Uterus

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References

Abdelhafez HM, Amara TMMA, El-debsi SM. (2015). Evaluation of Role of Gliben clamide and Aphanizome non flos-aquae Extraxt on Lymph Node and Spleen of Diabetic Rats. The Egyptian Journal of Hospital Medicine. Vol 61: 700-720.

Abd-Elkareem MD. (2017). Morphological, Histological and Immunohistochemical Study of the Rabbit Uterus during Pseudopregnancy. Journal of Cytology & Histology. 8(1):1-7.

Adkar PP, Ghadge PM, Ambavade SB, Shelke TT, Bhaskar VH. (2014). Effect of hydroethanol extract of leaves of trapa bispinosa Roxb on Histology Assessment in Reproductive system of Albino Mice. Journal of Pharmacy Research. 8(4): 570-575.

Affah GU, Obeten KE, Obasee PP, Isaac VN. (2014). Extract of Ginger (Zingiber officinale) on the Histology of thw Spleen using Adult Male Rats. Journal Biology, Agriculture and Healthcare. 4(25): 259-267.

Al-Qudsi F, Linjawi S. (2012). Hostological and Hormonal Changes in Rat Endometrium under the Effect of Camphor (Cinnamomom camphor). Life Science Journal. 9(2): 348-355.

Amabe O, Akpantah, Moses BE, Kelechi CU, Mfon AMBB, Mokutima AE, Theresa BE. (2010). Gonadal histo-morphologies and serum hormonal milieu in female rats treates with Azadirachta indica leaf extract. Iranian Journal of Reproductive Medicine. 8(4): 185-190.

Arsad SS, Esa NM, Hamzah H. (2014). Histopathologic Changes in Liver and Kidney Tissues from Male Sprague Dawley Rats Treated with Rhaphidophora Decursiva (Roxb.) Schott Extract. Journal of Cytology & Histology. S4: 001. doi: 10.4172/2157-7099.S4-001.

Aspelund A, Robciuc MR, Karaman S, Makinen T, Alitalo K. (2016). Lymphatic System in Cardiovascular Medicine. (Circ Res. 2016;118:515-530. DOI: 10.1161/CIRCRESAHA.115.306544.)

Assiam N, Iriani S, Sang KS. (2014). Pengaruh Dosis dan Lama Perlakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrus Meissn.) Terhadap Struktur Histologi Ginjal Mencit (Mus musculus L.). Jurnal Simbiosis II(2): 236-246.

Brosens, Pijinenborg, Benagiano. (2013). Defective myometrial spiral artery remodeling as a cause of major obsterical syndromes in endometriosis and adenomyosis. Placenta. 34: 100-105.

Cottencin O, Rolland B, Karila L. (2014). New Designer Drugs (synthetic cannabinoids and synthetic cathinones): review of literature. Current Pharmaceutical Design.20 (25):4106-4111.

EISAI. (1995). Medical Herbs Index in Indonesia. Jakarta.

Ekor M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology. Vol 14 (117): 1-10

El-Sherief A, Lau CT, Obuchowski NA, Mehta AC, Rice TW, Blackstone EH. (2017). Cross Disciplinary Analyisis of Lymph Node Classification in Lung Cancer on CT Scanning. CHEST. 151 (4): 776-785.

Faria SC, Sagebiel T, Balachandran A, Devine C, Lal C, Bhosale P. Imaginf in endometrial carcinoma. Indian Journal of Radiology and Imaging. 25(2): 137-147.

Fitmawati, Sofiyanti N, Roza RM, Isnaini, Hazimi H, Irawab YR, Winata DR, Dewi APK. (2017a). Traditional medicinal formulation: Obat pahit from Lingga Malay Ethnic in Riau Archipelago, Indonesia. Bidiversitas. Vol 18 (3): 1196-1200.

Fitmawati, Roza RM, Sofiyanti N, Isnaini, Fitri FL, Paramita D, Dewi APK. (2017b). Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Ratus novergicus). Biosaintifika. Vol 9 (3): 430-436.

Husain M, Makiyah SN. (2012). Pengaruh Pajanag Gelombang Telepon Seluler Terhadap Struktur Histologi Limpa pada Mencit (Mus musculus). Jurnal Kedokteran Yarsi. 20(3):167-173.

Liao S, Weid PY. (2016). Lymphatic System: An Active Pathway for Immune Protection. Semin Cell Dev Biol. Vol 38: 83-39.

Margaris KN, Black RA. (2012). Modelling the lymphatic system: challenges and opporunities. Journal of The Royal Society Interface. Vol 9: 601-612.

Matheos C, Lintong P, Kairupan C. (2013). Gambaran Histologik Jaringan Limpa Tikus Putih (Rattus norvegicus) yang Diinfeksi Eschericia coli dan Diberi Madu. Jurnal e-Biomedik (eBM). 1(2): 961-965.

Omar NA. (2012). Effect of some Aflatoxins on a Lymphatic Organ (Spleen) of Male Albino Rats (Histopathological Study). The Egyptian Journal of Hospital Medicine. Vol 48: 357-367.

Parasuraman S. (2011). Toxicology screening. Journal of Pharmacology & Pharmacotherapeutics. 2(2): 74-79.

Prakash, O., Kumar, A., & Kumar, P. (2013). Screening of Analgesic and Immunomodulator activity of Artocarpus heterophyllus Lam. Leaves (Jackfruit) in Mice. Journal of Pharmacognosy and Phytochemestry, 1(6), 33-36.

Zuhud, E.A.M. dan A. Hikmat. (1998). Eksplorasi dan kemungkinan pengembangan tumbuhan hutan sebagai bahan obat. Makalah Utama dalam Diskusi Hasil Hutan Bukan Kayu.Pusat Penelitian dan Pengembangan Hasil Hutan dan Sosial Ekonomi Kehutanan. Bogor.

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