The Role of Acetylcholine Esterase in Resistance Mechanism of Plutella xylostella to Emamektin Benzoate

Udi Tarwotjo, Rully Rahadian


One of the resistance mechanism of P. xylostellato emamektin benzoate is target insensitivity which is acetylcholine esterase that responsible for resistance occurrence. The objective of this study was to determine the role of acetylcholinesterase in the resistance mechanism of P. xylostella population to emamektin benzoate. For enzyme activity analysis, larvae homogenate of the third instar of P. xylostella was prepared. The number of insects required for each scour is 1 for each field population. The protein content in P. xylostella homogenate was measured by the Folin-Ciocalteu test. Non-specific esterase activity with an absorption rate was read using ELISA reader tool with λ = 450 nm. The inhibition level of acetylcholinesterase activity by emamectin benzoate in the tested population was 36.84%. The highest inhibition occurs in Puasan (Ngablak) population.  The result shows that a α-naphthyl acetate substrate was used so that it was recorded as non-specific esterase activity and did not exhibit esterase activity which specifically describes emamectin benzoate. Non-specific esterase enzyme activity of either α or β-naphthyl acetate substances to benzoic emamectin in the tested population most of the population was still susceptible. On α-naphthyl acetate substrate, the highest absorbance value found in susceptible population to benzoate emamectin (0.773), while the lowest found in Babrik (Ngablak) population  (0.083).


Acetylcholinesterase; Emamectin benzoate; Plutella xylostella; Resistance

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Abdullah NMM, Joginder S. (2004). Effect of insecticides on longevity of the whitefly, Bemisia tabaci (Gennadius) on cotton. Univ Aden. J. Nat Appl Sci.8:261–268.

Benyamin, W.P. & Alphey, N. (2017 ). Resistance genetic insect control: Modelling the effects of space. Journal of Theoretical Biology. Vol . 413: 72-85.

Brengues, C., Hawkes, N.J., Chandre, F., McCarroll, L., Duchon, S., Guillet, P., Maguin, S., Morgan, J.C., Hemingway, J. (2003). Pyrethroid & DDT cross-resistance in Aedes aegypti correlated with novel mutations in the voltage-gated sodium channel gene. Medical & Veterinary Entomology 17:87–94.

Casimiro, S., Coleman, M., Hemingway, J., Sharp, B. (2006). Insecticide resistance in Anopheles arabiensis and Anopheles gambiae from Mozambique. J Med Entomol 43: 276-282.

Hemingway, J., Hawkes, N.J., & McCarroll, I., & Ranson, H. (2004).The Molecular Basis of Insecticides Resistance in Mosquitoes. Insect Biochemistry & Molecular Biol. 34: 653-665

Khan, H.A.A., Akram, W., Khan, T., Haider, M.S., Iqbal, N., Zubair, M. (2016 ). Risk assessment, cross-resistance potential, & biochemical mechanism of resistance to emamectin benzoate in a field strain of house fly (Musca domestica Linnaeus).

Lee, H.L., (1991). Esterase Activity & Temephos Susceptibility in Aedes aegypti(L) Larvae.Mosquito-Borne Disease Bull. 8: 91-94.

Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951).Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem 193: 265-275.

Li, D., Xu, L., Pang, S ., Liu, Z., Zhao, W., & Chengju Wang. C. (2017). Multiple pesticides detoxification functions of maize (Zea mays) GST34. J. Agric. Food Chem., 65 (9), pp 1847–1853.

Nuessly, G.S., Scully, B.T., Hentz, M.G., Beiriger, R., Snook, M.E., Widstrom, N.W. (2007). Resistance to Spodoptera frugiperda (Lepidoptera: Noctuidae) & Euxesta stigmatias (Diptera: Ulidiidae) in sweet corn derived from exogenous & endogenous genetic systems. J. Econ Entomol. 100:1887–1895

Rhee, I.K., Meent, K., Ingkanian, K., Verpoorte, R. 2001. Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. J. Chromatogr. A, 915: 217-223.

Roush, R.T., & Miller, G.L. 2005. Conciderations for Design of Insecticide Resistance Monitoring Program. .J. Econ. Entomol. 79: 293-298

Sasim, M.S., Egiert, J.S., & Kosakowaska, A. (2014).Quantitative analysis of extracted phycobilin pigments in cyanobacteria—an assessment of spectrophotometric & spectrofluorometric methods. J. Appl Phycol 26(5)2065-2074.

Smagghe, G. 2004. Synergism of Diacylhydrazine Insecticides with Metyrapone & Diethylmaleate.Blackwell Verlag, Berlin. JEN 128: 465-468.

Smirle, M.J., Zurowski, C.L., Lowery, D.T., Foottit, R.G. (2010). Relationship of insecticide tolerance to esterase enzyme activity in Aphis pomi & Aphis spiraecola. Journal of Economic Entomology. 103:374–378.

Tarwotjo, U. & Rahadian, R. (2017). Resistance inheritance of Plutella xylostella toresidual of emamectin benzoate. Biosaintifika: Journal of Biology & Biology Education, 9(1) 19-25

Walsh, S.B., Dolden, T.A., Moores,G.D., Kristensen, M., Lewis, T., Devonshire, A.L., & Wiliamsom, M.S. (2001). Identification & characterization of mutations in housefly (Musca domestica) acetylcholinesterase involved in insecticide resistance. J. Biochem. 359 : 175-181.

Wang, R., Tang. X.C. (2005). “Neuroprotective effects of huperzine A. A natural cholinesterase inhibitor for the treatment of Alzheimer’s disease”. Neuro-Signals. 14 (1–2): 71–82.

Young, J., Carlos, B., Miguez, & Byong, H.L. (2004). Characterization & Heterologous Gene Expression of a Novel Esterase from Lactobacillus casei CL96, Journal Appl Environ Microbiol.70 (6): 3213-3221



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