Callus Induction of Piper betle L. Var Nigra Using 2,4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin

Junairiah Junairiah, Edy Setiti Wida Utami, Ni’matuzahroh Ni’matuzahroh, Lilis Sulistyorini

Abstract


Piper betle L var Nigra (black betel) is a member of Piperaceae family which has potential as medicinal plant due to secondary metabolites it contained. Callus culture is one of the alternative methods to elevate production of secondary metabolites. This study was aimed to determine the effect of 2,4-dichlorofenoxyacetic acid (2,4-D) and 6-benzil aminopurin (BAP) towards callus induction and growth, also to determine the most optimal variation of 2,4-D and BAPconcentration for callus induction of black betel leaf explant. This study was a laboratory experimental study with complete randomized design. Black betel leaf explant was planted in Murashige and Skoog (MS) medium supplemented with 2,4-D and BAPgrowth regulators at concentration of 0.0 mg/L, 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L respectively. Parameter recorded for callus induction and growth including callus induction time (days), percentage of explant forming callus, fresh weight and dry weight, color and texture. After callus planted for 8 weeks, analysis was performed statistically. Result showed that 2,4-D and BAPsupplementation to medium affected the growth of black betel leaf explants. Additional concentration of 0.5 mg/L 2,4-D and 1.0 mg/L BAP growth regulators showed the fastest response in callus formation, at 7.25 days. Growth regulators of 2,4-D 0.5 mg/L and BAP 2.0 mg/L concentration produced the highest fresh and dry weight, at 0.6802 g and 0.0670 g respectively. The best treatment was used as a basis to produce secondary metabolites using elicitor


Keywords


Callus induction; Piper betle L.; 2,4-diclorofenolacetic acid; 6-benzil aminopurin



DOI: https://doi.org/10.15294/biosaintifika.v10i3.15962

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Biosaintifika: Journal of Biology & Biology Education by Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang is licensed under a Creative Commons Attribution 4.0 International Licensep-ISSN (Print) 2085-191X | e-ISSN (Online) 2338-7610