Blood Hematological and Biochemical Parameters of Osteochilus vittatus with Spirulina platensis Supplementation in Biofloc System

Muhammad Azharul Rijal, Susanto Susanto, Sorta Basar Ida Simanjuntak, Hernayanti Hernayanti, Purnama Sukardi

Abstract


Spirulina platensis is a microalgae that contains nutrients such us iron and phycocianin higher than other microalgae. Research on suplementation of S. platensis in Osteochilus vittatus cultured in biofloc system has never been done. The aim of this study was to determine the blood hematological and biochemical parameters of O. vittatus fed with S. platensis supplementation and maintained in biofloc system. The study was conducted experimentally with Completely Randomized Design, four treatments and five replications. O. vittatus were fed with S. platensis level 0, 2, 4, and 6 g.kg-1 for 56 days. Blood hematological and biochemical parameters were measured on days 0 and 56. Values of those parameters were then analyzed using ANOVA with a confidence level of 95%. The result showed that the highest red blood cells, white blood cells, and hemoglobin counts were 1.57x106­­­­ cell.mm-3, 2.37x105 cell.mm-3, and 6.77 g.dl-1 respectivley, while the highest hematocrit value was 17.5 %. The highest total protein, albumin, and globulin in blood were 7.96 g.dL-1, 4.31 g.dL-1, and 3.79 g.dL-1 respectively, and the best for ratio A/G was 1.14. S. platensis suplementation level of 4g.kg-1 feed was the most optimum level (P<0.05). Cultivation of fish with S. platensis supplementation in feed can increase fish health indicated by the hematological and biochemical parameters of the blood and the fish maintenance in biofloc systems can improve water quality. The benefit of this research is to make a good condition for cultivation and efficiently used not only water but also feed.


Keywords


biofloc; biochemical; hematological; Ostechilus vittatus; Spirulina platensis

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References


Adel, M., Yeganeh, S., Dadar, M., Sakai, M., & Dawood, M.A.O. (2016). Effect of Dietary Spirulina platensis on Growth Performence, Humoral and Mucosal Imune Response and Disease Resistence in Juvenile great sturgeon (Huso huso). Fish and Shellfish Imunology, III(12), 436-444.

Ahmed, S.M., Ali, H.A. (2013). Serum protein and leucocytes differential count in the common carp (Cryprinus carpio L) infested with ecto-paracites. Mesopot Journal Marine Science, 28(2), 151-162.

Anand, P.S.S., Kohli, M.P.S., Kumar S., Sundaray, J.K., Dam Roy, S., Venkateshwarlu, G., Sinha A., Pailan, G.H. (2014). Effect of dietary sup-plementation of biofloc on growth performance and digestive activities in Panaeus monodon. Aquaculture , 418 (2), 108-115.

Cifferri O., Tiboni, O. (1985). The Biochemistry and industrial potential of Spirulina. Annu. Rev. Microbiology, 39(2), 503-526.

Dernekbasi, S., Unal, H., Karayucel, I., Aral, O. (2010). Effect of dietary supplemnetation of different rates of Spirulina platensis on growth and feed convertion in Guppy (Poecilia reticu-lata P). Journal of Animal and Veterinary Ad-vances, 9(9), 1395-1399.

Guardiola, F.A., Bahi, A., Esteban, M.A. (2018). Effect of dietary administration of fenugreek seeds on metabolic parameters and immune sta-tus of gilthead seabream (Sparus aurata L). Fish and Shellfish Immunology, 23(2), 34-40.

Hosseini S.M., Khosravi-Darani, K., Mozafari, M.R. (2013). Nutritial and medical applications of Spirulina platensis microalgae. Mini Rev. Medical. Chemistry, 8(13), 1231-1237.

Kheti, B., Kamilya, D., Choudhury, J., Parhi, J., Debbarma, M., Singh, S.T. (2017). Dietary mi-crobial floc potentiates immune respone, im-mune relevant gene experssion and disease re-sistance in Rohu, Labeo rohita finggerlings. Aquaculture, 468(1), 501-507.

Kumar, S., Raman, R.P., Prasad, K., Srivastva, P., Kumar, S., Rajendran, K.V. (2017). Modula-tion of innate immune responses and induction of oxidative stress biomarkers in Pangasiano-don hypothalamus following an experimental infection with dactylogrid manogenean. Fish and Shellfish Immunology, 34(5), 384-890.

Liu, Q., Huang, Y., Zhang, R., Cai, T. and Cai, Y. (2016). Medical Application of Spirulina platensis Derived CPhycocyanin. Hindawi Publishing Corporation, Evidence-Based Complementary and Alternative Medicine, 14,p.

Norousta, R., Mousavi-Sabet, H. (2013). Com-paarative characerization of blood cells and hematological parameters between the imma-ture Caspian Vimba (Vimba vimba). AACL BIo-flux, 6(3), 232-240.

Promya J., Chitmanat, C. (2011). The effect of Spirulina platensis and Cladophora algae on the growth performence, meat quality and im-munity stimulating capacity of the african sharptooth catfish (Clarias garipenus). Interna-tional Journal of Agriculture and Biology, 13(2), 77-82.

Raji A.A., Alaba, P.A., Yusuf, H., Abu B.N.H., Taufek, N.M., Muin, H., Alias, Z., Milow, P., Razak, S.A. (2018). Fishmeal replacement with Spirulina platensis and Chorella vulgaris in af-rican catfish (Clarias garipenus) diet : Effect on antioxidant enzyme activities and haemoto-logical parameters. Reseacrh in Veterniery Sci-ence., 119(4), 67-75.

Rani, Y., Retno, B.N., Yuniarti, A., Rana, P. The effectiveness of Dunaliella salina against the decrease macronuclei and micronuclei of hybrid grouper (Epinephelus fuscogustatus x Epinephelus lanceolatus) infected by iridovirus. RJOAS, 10(82): 245-249.

Rehulka, J. (1993). Erythrodematitis of carp, Cy-prinus carpio (L.) : An electrophoretic study of blood serum protein fraction dosis. Acta Vet. Brno, 60(93): 187-197.

Satyantini, W.H., Sukenda, Harris, E., Utomo, N.B.P. (2014). Pemberian fikosianin Spirulina meningkatkan jumlah sel darah, aktivitas fagos-itosis, dan pertumbuhan ikan kerapu bebek. Jurnal Veternier, 15(1), 46-56.

Simanjuntak, S.B.I., Wibowo, E.S., Indarmawan. (2016). Stimulation of deprivantion sycles with Spirulina platensis feed supplementation on (Osphronemus gouramy) physiological re-sponses. Biosaintifika, 8(3), 377-384.

Simanjuntak, S.B.I., Wibowo, E.S., Indarmawan. (2018). Impact of feed containing different do-sis of diets supplementation Spirulina platensis on growth, haematological, body composition and biochemical parameters of Gurami (Osphronemus gouramy). Turkish Journal of Fisheries and Aquatic Science, 18(4), 681-690.

Sitorious, T. G., Sotiroudis G. T. (2013). Health aspect of Spirulina (Arthrospira) microalgae food supplement. J. Serb. Chem. Soc., 78(3), 395-405.

Sumarma, A., Budihastudi, D.W., Sistina, Y. (2010). Penggunaan ekstender madu yang dikombinasikan dengan krioprotektan berbeda pada pengawetan sperma ikan nilem (Osteochi-lus vittatus). Omni-Akuatika, 9(11), 51-55.

Yageneh, S.M. (2014). Dietary effect of Spirulina platensis on hematological dan serum biochem-ical parameters of rainbow trout (Oncorhyn-chus mykiss). Research in Veterinary Science, 6(11), 34-41.

Yu, W., Wen G., Lin H., Yang, Y., Huang, X., Zhou, C., Zhang, Z., Duan Y., Huang Z., Li, T. (2018). Effect of dietary Spirulina platensis on growth performance, hematological and serum biochemical parametres, hepatic antioxidant status, immune responses and disease resist-ence of Coral trout Plectropomus leopardus L. Fish and Shellfish Immunoly., 74(13), 649-655.

Yusuf, D., Yusuf., Sugiharto., Wijayanti, G.E. (2014). Perkembangan Post larva ikan nilem (Osteochilus vittatus) dengan pola pemberian pakan berbeda. Scipta Biologica, 1(3), 185-193.




DOI: https://doi.org/10.15294/biosaintifika.v12i3.20936

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