Larvicidal Activity of Brugmansia candida against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

Setiawan Khoirul Himmi, Didi Tarmadi, Dita Meisyara, Anugerah Fajar, Titik Kartika, Ikhsan Guswenrivo, Sulaeman Yusuf


Mosquitoes are well known as vectors of hazardous diseases for human. Plant extracts can be used as an alternative for larval control due to they are a rich source of bioactive chemicals and safe for the environment. The present study investigated the larvicidal activity of crude extracts derived from leaf and flower of Brugmansia candida against the second larval instar of Aedes aegypti and Culex quinquefasciatus. The larval mortality was observed at 24 and 48 h exposure of both leaf and flower extracts, at the concentration of 100, 250, 500, and 1000 ppm. The 24 h exposure of both extracts at the concentration of 500 and 1000 ppm resulted in larval mortality rates were significantly lower than those of 48 h exposure. However, the mortality rate was not significantly different at the lower concentrations of crude extracts. The results also suggested that there was no significant difference in the larvicidal effect between leaf and flower extracts at 24 and 48 h exposure for all concentrations. The LC50 values at 48 h exposure for leaf extract were 789 and 791 ppm for Ae. aegypti and C. quinquefasciatus, respectively, whereas for flower extract were 772 and 780 ppm for Ae. aegypti and C. quinquefasciatus, respectively. Overall, B. candida showed larvicidal activity against Ae. aegypti and C. quinquefasciatus. This research contributes to new finding regarding the larvicidal activity of B. candida. This finding also supports the next study to develop B. candida as an alternative source for larval control agent.


Brugmansia candida; Crude Extract; Larval Mortality; Mosquitoes

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Cardillo, A. B., Otálvaro, A. M., Busto, V. D., Talou, J. R., Velásquez, M. E., & Giulietti, A. M. (2010). Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors. Process Biochemistry, 45(9), 1577-1581.

Changbunjong, T., Wongwit, W., Leemingsawat, S., Tongtokit, Y., Deesin, V. (2010). Effect of crude extract of Solanum xanthocarpum against snails and mosquito larvae. Southeast Asian Journal of Tropical Medicine and Public Health, 41(2), 320-325.

Dehkordi, A.S., Vatandoost, H., Abaei, M.R., Davari, B., Sedaghat, M.M. (2016). Chemical composition and larvicidal activity of Bunium persicum essential oil against two important mosquitoes vectors. Journal of Essensial Oil Bearing Plants, 19(2), 349-357.

Duke, S. O., Cantrell, C. L., Meepagala, K. M., Wedge, D. E., Tabanca, N., Schrader, K. K. (2010). Natural toxins for use in pest management. Toxins, 2(8), 1943-1962.

Ghananand, T., Prasad, C.S., Lok, N. (2011). Effect of insecticides, bio-pesticides and botanicals on the population of natural enemies in brinjal ecosystem. Vegetos: An International Journal of Plant Research, 24(2), 40- 44.

Govindarajan, M. and Karuppannan, P. (2011). Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine, 4(1), 24-28.

Govindarajan, M., Sivakumar, R., Rajeswary, M., Yogalakshmi, K. (2012). Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitology Research, 110, 2022-2032.

Govindarajan, M., Sivakumar, R., Rajeswary, M., Yogalakshmi, K. (2013). Chemical composition and larvicidal activity of essential oil from Ocimum basilicum (L.) against Culex tritaeniorhynchus, Aedes albopictus and Anopheles subpictus (Diptera: Culicidae). Experimental Parasitology, 134(1), 7-11.

Ismayati, M., Zulfiana, D., Tarmadi, D., Lestari, A.S., Krishanti, N.P.R.A., Himmi, S.K., Fajar, A., Yusuf, S. (2019). Biological control of wood destroying organism using plant extracts collected from Mt. Merapi National Park, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 11(3), 360-368.

Kerchner, A., and Farkas, A. (2020). Worldwide poisoning potential of Brugmansia and Datura. Forensic Toxicology, 38, 30-41.

Meenakshi, S.V., Jayaprakash, K. (2014). Mosquito larvicidal efficacy of leaf extract from mangrove plant Rhizophora mucronata (Family: Rhizophoraceae) against Anopheles and Aedes species. Journal of Pharmacognosy and Phytochemistry, 3(1), 78-83.

Nour, A.H., Sandanasamy, J., Nour, A.H. (2012). Larvicidal activity of extracts from different parts of Neem (Azadirachta indica) against Aedes aegypti mosquitoes’ larvae. Scientific Research and Essays, 7(31), 2810-2815.

Pavela, R., Vrchotová, N., Tříska, J. (2016). Larvicidal activity of extracts from Ammi visnaga Linn. (Apiaceae) seeds against Culex quinquefasciatus Say. (Diptera: Culicidae). Experimental Parasitology, 165, 51-57.

Rawani, A., Ghosh, A., Chandra, G. (2010). Mosquito larvicidal activities of Solanum nigrum L. leaf extract against Culex quinquefasciatus Say. Parasitology Research, 107, 1235–1240.

Samy, A.M., Elaagip, A.H., Kenawy, M.A., Ayres, C.F.J., Peterson, A.T., Soliman, D.E. (2016). Climate change influences on the global potential distribution of the mosquito Culex quinquefasciatus, vector of west nile virus and lymphatic filariasis. PloS One, 11, e0163863.

Santhoshkumar, T., Rahuman, A.A., Rajakumar, G., Marimuthu, S., Bagavan, A., Jayaseelan, C., Zahir, A.A., Elango, G., Kamaraj, C. (2010). Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitology Research, 108, 693–702.

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., Latha, L. Y. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1-10.

Sundaravadivelan, C., Padmanabhan., M.N., Sivaprasath, P., Kishmu, L. (2013). Biosynthesized silver nanoparticles from Pedilanthus tithymaloides leaf extract with anti-developmental activity against larval instars of Aedes aegypti L. (Diptera; Culicidae). Parasitology Research, 112, 303-311.

Tarmadi, D., Gunandini, D.J. and Yusuf, S. (2018). Larvicidal activity of Cerbera odollam Gaertn against a dengue vector, Aedes aegypti (Diptera: Culicidae). Sustainable Future for Human Security. Singapore: Springer.

Tarmadi D., Himmi, S.K., Yusuf, S. (2014). Termiticidal activity of an extract of Brugmansia candida leaf against a subterranean termite Copotermes gestroi Wasmann and a drywood termite Cryptotermes cynocephalus Light. Proceedings of the 10th Pacific-Termite Research Group Conference, Kuala Lumpur, Malaysia, 26-28 February 2014. pp. S3:4.

Ujihara K. (2019). The history of extensive structural modifications of pyrethroids. Journal of Pesticide Science, 44(4), 215–224.

Vivekanandhan, P., Nathan, S.S., Shivakumar, M.S. (2018). Larvicidal, pupicidal and adult smoke toxic effects of Acanthospermum hispidum (DC) leaf crude extracts against mosquito vectors. Physiological and Molecular Plant Pathology, 101, 156-162.



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