The Best Inoculation Technique Applied on Gyrinops versteegii Tree Trunk

Resti Wahyuni(1), Amalia Indah Prihantini(2),


(1) Center for Research and Development of Environmental Quality and Laboratory
(2) Non Timber Forest Product Technology Research and Development Institute

Abstract

Agarwood is a non-timber forest product having high economic value, however, its population in nature is getting decrease. Gyrinops versteegii, an agarwood-producing plant, has been listed in Appendix II CITES, therefore, exploitation of the agarwood in nature must be reduced. An effort to reduce the agarwood exploitation the nature is by agarwood cultivation including cultivation of agarwood-producing plant and agarwood inoculation. Some of agarwood inoculation methods are simpori and implant techniques. Simpori is a modified inoculation method using porous nail and Fusarium solani, meanwhile implant technique uses drill and pieces of wood that is soaked in F. solani spores. The present study aimed to determine the best inoculation technique applied to G. versteegii tree trunk at 4 to 5m height, comparing simpori and implant techniques. This study used completely randomizes design with two treatments (simpori and implant techniques). The result showed that the averages yield of production agarwood produced by simpori and implant techniques are 0.66 and 0.64%, respectively. Based on SNI 7631:2011, the visual quality of the produced agarwood belongs to kemedangan TG.C. In conclusion, both simpori and implant technique produced agarwood in similar quality and quantity at six months inoculation, however, simpori is more practical for application in the field. This study provides additional data about agarwood produced by simpori and implant technique and give the alternative methods for agarwood farmers producing their agarwood themselves.

Keywords

Agarwood; Implant; Simpori

Full Text:

PDF

References

Abdullah, A., Nik Ismail, N. K., Abdul Kadir, T. A., Md Zain, J., Jusoh, N. A., & Mohd Ali, N. (2007). Agar Wood Grade Determination System Using Image Processing Technique. Proceedings of the International Conference on Electrical Engineering and Informatics, 427–429.

Akter, S., Islam, M. T., Zulkefeli, M., & Khan, S. I. (2013). Agarwood production a multidisiplinary field to be explored in Bangladesh. International Journal of Pharmaceutical and Life Sciences, 2(1), 22–32.

Amin, M. R. M., Bejo, S. K., Ismail, W. I. W., & Mashohor, S. (2012). Colour extraction of agarwood images for fuzzy C-means classification. Walailak Journal of Science and Technology, 9(4), 445–459.

Anggadhania, L., Nugraheni, Y. M. M. A., Suryawan, I. G., & Sutariningsih, E. (2014). Peningkatan strain kapang pembentuka gaharu pada jenis Gyrinops verstegii di NTB.

Azah, M., Husni, S. S., Mailina, J., Sahrim, L., Majid, J. A., & Faridz, Z. M. (2013). Classification of Agarwood ( Gaharu ) By Resin Content. Journal of Tropical Forest Science, 25(2), 213–219.

Compton, J., & Ishihara, A. (2004). The use and trade of agarwood in Japan. In A TRAFFIC report to the CITES Secretariat (Vol. 6). http://144.76.93.178/sites/default/files/common/com/pc/15/X-PC15-06-Inf.pdf

Faizal, A., Esyanti, R. R., Aulianisa, E. N., Iriawati, Santoso, E., & Turjaman, M. (2017). Formation of agarwood from Aquilaria malaccensis in response to inoculation of local strains of Fusarium solani. Trees - Structure and Function, 31(1), 189–197. https://doi.org/10.1007/s00468-016-1471-9

Kakino, M., Tazawa, S., Maruyama, H., Tsuruma, K., Araki, Y., Shimazawa, M., & Hara, H. (2010). Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complementary and Alternative Medicine, 10(1), 68–75. https://doi.org/10.1186/1472-6882-10-68

Karlinasari, L., Indahsuary, N., Santoso, E., Turjaman, M., & Nandika, D. (2015). Sonic and Ultrasonic Waves in Agarwood Trees (Aquilaria microcarpa) Inoculated with Fusarium solani. Journal of Tropical Forest Sciense, 27(3), 351–356.

Li, W., Cai, C. H., Guo, Z. K., Wang, H., Zuo, W. J., Dong, W. H., Mei, W. L., & Dai, H. F. (2015). Five new eudesmane-type sesquiterpenoids from Chinese agarwood induced by artificial holing. Fitoterapia, 100, 44–49. https://doi.org/10.1016/j.fitote.2014.11.010

Liu, Y., Chen, H., Yang, Y., Zhang, Z., Wei, J., Meng, H., Chen, W., Feng, J., Gan, B., Chen, X., Gao, Z., Huang, J., Chen, B., & Chen, H. (2013). Whole-tree agarwood-inducing technique: an efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees. Molecules (Basel, Switzerland), 18(3), 3086–3106. https://doi.org/10.3390/molecules18033086

López-Sampson, A., & Page, T. (2018). History of Use and Trade of Agarwood. Economic Botany, 72(1), 107–129. https://doi.org/10.1007/s12231-018-9408-4

Mohamed, R., Jong, P. L., & Irdayu, I. N. (2014). Succession patterns of fungi associated to wound-induced agarwood in wild Aquilaria malaccensis revealed from quantitative PCR assay. World Journal of Microbiology and Biotechnology, 30(9), 2427–2436. https://doi.org/10.1007/s11274-014-1668-2

Mohamed, R., Jong, P. L., & Kamziah, A. K. (2014). Fungal inoculation induced agarwood in young Aquilaria malaccensis trees in the nursery. Journal of Forestry Research, 25(1), 201–204.

Mucharromah. (2010). Pengembangan Gaharu di Bengkulu, Sumatera (The Development of Agarwood in Bengkulu, Sumatra). Info Hutan, 7(2), 117–128.

Naziz, P. S., Das, R., & Sen, S. (2019). The scent of stress: Evidence from the unique fragrance of agarwood. Frontiers in Plant Science, 10, 1–13. https://doi.org/10.3389/fpls.2019.00840

Nurbaya, Kuswinanti, T., Rosmana, A., & Millang, S. (2014). Growth rate and identification of Fusarium spp . assosiated with Aquillaria spp . from Nunukan regency , North Kalimantan. International Journal of Current Research and Academic Review, 2(11), 33–40.

Okudera, Y., & Ito, M. (2009). Production of agarwood fragrant constituents in Aquilaria calli and cell suspension cultures. Plant Biotechnology, 26(3), 307–315. https://doi.org/10.5511/plantbiotechnology.26.307

Roemantyo, R., & Partomihardjo, T. (2010). Analisis Prediksi Sebaran Alami Gaharu Marga Aquilaria dan Gyrinops di Indonesia [Natural Distribution Prediction Analyses of Agarwood Genera of Aquilaria and Gyrinops in Indonesia]. Berita Biologi, 10(2), 189–198. https://doi.org/10.14203/beritabiologi.v10i2.1972

Subasinghe, S. M. C. U. P., & Hettiarachchi, D. S. (2013). Agarwood resin production and resin quality of Gyrinops walla Gaertn. Journal of Agricultural Sciences, 3(1), 357–362.

Subasinghe, S. M. C. U. P., Hettiarachchi, D. S., & Rathnamalala, E. (2012). Agarwood-type Resin from Gyrinops walla Gaertn: A New Discovery. Journal of Tropical Forestry and Environment, 2(2), 43–48. https://doi.org/10.31357/jtfe.v2i2.594

Subasinghe, S. M. C. U. P., Hitihamu, H. I. D., & Fernando, K. M. E. P. (2019). Use of two fungal species to induce agarwood resin formation in Gyrinops walla. Journal of Forestry Research, 30(2), 721–726. https://doi.org/10.1007/s11676-018-0654-1

Van Thanh, L., Van Do, T., Son, N. H., Sato, T., & Kozan, O. (2015). Impacts of biological, chemical and mechanical treatments on sesquiterpene content in stems of planted Aquilaria crassna trees. Agroforestry Systems, 89(6), 973–981. https://doi.org/10.1007/s10457-015-9829-3

Wahyuni, R, Prihantini, A., Mansyur, & Ramdiawan. (2018). Laporan hasil pengembangan pilot iptek inokulasi gaharu.

Wahyuni, Resti, Prihantini, A. I., & Anggadhania, L. (2020). Formation of Gyrinops versteegii Agarwood by Fusarium solani Bioinduction with Simpori Technique. Jurnal Ilmu Pertanian Indonesia, 25(1), 152–159. https://doi.org/10.18343/jipi.25.1.152

Wahyuni, Resti, Triadiati, T., & Falah, S. (2018). Induction of agarwood in Aquilaria malaccensis using nitrogen fertilizer and Fusarium solani. Jurnal Penelitian Kehutanan Wallacea, 7(2), 165–171. https://doi.org/10.18330/jwallacea.2018.vol7iss2pp165-171

Wong, M. T., Siah, C. H., Faridah, Q. Z., & Mohamed, R. (2013). Characterization of wound responsive genes in Aquilaria malaccensis. Journal of Plant Biochemistry and Biotechnology, 22(2), 168–175. https://doi.org/10.1007/s13562-012-0144-z

Xu, Y., Zhang, Z., Wang, M., Wei, J., Chen, H., Gao, Z., Sui, C., Luo, H., Zhang, X., Yang, Y., Meng, H., & Li, W. (2013). Identification of genes related to agarwood formation: transcriptome analysis of healthy and wounded tissues of Aquilaria sinensis. BMC Genomics, 14, 227–242. https://doi.org/10.1186/1471-2164-14-227

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.