The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME

Yohanes Bernard Subowo(1), Arwan Sugiharto(2),

DOI: https://doi.org/10.15294/biosaintifika.v13i2.29660


(1) Microbiology Department, Centre Research of Biology, The Indonesian Institute of Sciences
(2) Microbiology Department, Centre Research of Biology, The Indonesian Institute of Sciences, Indonesia

Abstract

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME.

 

Keywords

POME degradation; inducer; laccase; Coltricia cinnamomea

Full Text:

PDF

References

Asgher, M. (2011). Characterization of a novel manganese peroxidase purified from solid state culture of Trametes versicolor IBL-04. Bioresources 6, 4317–4330.

Bala, J.D., Lalung, J., Al-Gheethi, A.A.S., Kaizar, H. & Ismail, N. (2018). Reduction of Organic Load and Biodegradation of Palm Oil Mill Effluent by Aerobic Indigenous Mixed Microbial Consortium Isolated from Palm Oil Mill Effluent (POME). Water Conserv Sci Eng, 18 pp. https://doi.org/10.1007/s41101-018-0043-9.

Bettin, F., Montanari, Q., Calloni, R., Gaio, T.A., Silveira, M.M., & Dillon, A.J.P. (2014). Additive effect of CuSO4 and aromatic compounds on laccase production by Pleurotus sajor-caju PS-2001 using sucrose as a Carbon source. Brazilian Journal of Chemical Engineering, 31(2), 335-346.

Baharuddin, A.S., Hock, L.S., Yusof, M.Z., & Rahman, N.A.A. (2010). Effects of palm oil mill effluent (POME) anaerobic sludge from 500 m3 of closed anaerobic methane digested tank on pressed-shredded empty fruit bunch (EFB) composting process. African Journal of Biotechnology 9 (16), 2427-2436.

Dana, M., Khaniki, G.B., Mokhtarieh, A.A., Davarpanah, S.J. (2017). Biotechnological and Industrial Applications of Laccase: A Review. Journal of Applied Biotechnology Reports, 4 (4), 675-679.

Dhakar, K., & Pandey, A. (2013). Laccase Production from a Temperature and pH Tolerant Fungal Strain of Trametes hirsuta (MTCC 11397). Hindawi Publishing Corporation Enzyme Research, 9 pp.

Glenn, J. K., & Gold, M. H. (1983). Decolorization of several polymeric dyes by the lignin–degrading Basidiomycetes Phanerochaete chrysosporium. Appl. Environ Microbiol. 45(6), 1741-1747

Gonzalez, J.C., Medina, S.C., Rodriguez, A., Osma, J.F., & Almeciga-Diaz, C.J. (2013). Production of Trametes pubescens laccase under submerged and semi-solid culture conditions on agroindustrial wastes. PLOS ONE. 8 (9), 1-14.

Hu, J., Zhang, Y., Xu, Y., Sun, Q., Liu, J., Fang, W., Xiao, Y., Kues, U., & Fang, Z. (2019). Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction. Appl Microbiol Biotechnol. 103, 411–425.

Irvan, Trisakti, B., Vincent, M., & Tandean, Y. (2012). Further processing of palm oil liquid waste used effective microorganism aerobically in order to reduce of TSS value. Jurnal Teknik Kimia USU 1 (2), 27-30.

Kocyigit, A., Yasa, I., Pazarbasi, M.B., & Ozdemir, G. (2012). Production of laccase from Trametes trogii TEM H2: a newly isolated white rot fungus by air sampling. Journal of Basic Microbiology 52, 1–9.

Kuo, M. (2010). Coltricia cinnamomea. Retrieved from the Mushroom Expert.Com Web site: http://www.mushroomexpert.com/coltricia_cinnamomea.html

Lanka, S., & Pydipalli, M. (2018). Reduction of organic load from palm oil mill effluent (POME) using selected fungal strains isolated from POME dump sites. African Journal of Biotechnology, 17 (36), 1138-1145.

Moreira, M.T., Viacava, C., & Vidal, G. (2004). Fed-batchdecolorization of poly R-478 by Trametes versicolor. Brazilian Archives of Biology and Technology 47(2),179-183.

Neoh, C.H., Lam, C.Y., Lim, C.K., Yahya, A., Ibrahim, Z. (2013). Decolorization of palm oil mill effluent using growing cultures of Curvularia clavata. Environ Sci Pollut Res, 12 pp.

Papinutti, V.L., Diorio, L.A., & Forchiassin, F. (2003). Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran. J. Ind. Microbiol. Biotechnol. 30, 157-160.

Piscitelli, A., Giardina, P., Lettera, V., Pezzella, C., Sannia, G., & Faraco, V. (2011).

Induction and Transcriptional Regulation of Laccases in Fungi. Current Genomics, 12, 104-112.

Rajeswari, M., & Bhuvaneswari, V. (2016). Production of extracellular laccase from the newly isolated Bacillus sp. PK4. African Journal of Biotechnology, 15 (34), 1813-1826.

Rao, M.A., Scelza, R., Acevedo, F., Diez, M.C., Gianfreda, L. (2014). Enzymes as useful tools for environmental purposes. Chemosphere, 107, 145-162.

Saraiva, J.A., Tavares, A.P.M., Xavier, A.M.R.B. (2012). Effect of the Inducers Veratryl Alcohol, Xylidine, and Ligninosulphonates on Activity and Thermal Stability and Inactivation Kinetics of Laccase from Trametes versicolor. Appl Biochem Biotechnol. 167, 685-693.

Schneider, W.D.H., Fontana, R.C., Mendonca, S., de Siqueira, F.G., Dillon, A.J.P., Camassola, M. (2018). High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources. Process Biochemistry, 69, 1-11.

Sitarz, A.K., Mikkelsen, J.D., & Meyer, A.S. (2016). Structure, functionality and tuning up of laccases for lignocellulose and other industrial applications. Critical Reviews in Biotechnology, 36(1), 70-86.

Songulashvili, G., Elishasvili, V., Wasser, S.P., Nevo, E., Hadar, Y. (2007). Basidiomycetes laccase and manganese peroxidase activity in submerged fermentation of food industry wastes. Enzyme and Microbial Technology 41, 57-61.

Soleimaninanadegani, M., & Manshad, S. (2014). Enhancement of Biodegradation of Palm Oil Mill Effluents by Local Isolated Microorganisms. Hindawi Publishing Corporation International Scholarly Research Notices, 8 pp.

Subowo, Y.B. (2017). The utilization of ink cap mushroom (Coprinus cinereus) on palm oil mill effluent degradation. Journal of Biological Researches 22 (21), 61-65.

Subowo, Y.B., & Sugiharto, A. (2019). The Interaction between Marasmius pulcheripes J8 and Soil Fungi on Laccase Activity for POME Degradation. Biosaintifika 11 (3): 178-184.

Taurisia, P.P., Proborini, M.W., Nurhantoro, I. (2015). The effect of media on the growth and biomass of fungi Alternaria alternata (Fries) Keissler. Jurnal Biologi 19 (1),30-33.

Tien, M. & Kirk, T.K. (1983). Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 221 (4611), 661–663.

Upadhyay, P., Shrivastava, R.R., Agrawal, P.K. (2016). Bioprospecting and biotechnological applications of fungal laccase. Biotech, 6(15), 12 pp.

Usha, K.Y., Praveen, K., & Reddy, B.R. (2014). Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea. Hindawi Publishing Corporation Biotechnology Research International, 9 pp.

Xia, C. Y., Zuo, J.L., Lu, J., Li, J., Chen, L. Z., Li, S.H., Tao, T., Li, T., Li, X., Wang, Y. L., Liu, Y. (2009). A spectrophotometric method for determination of chemical oxygen demand using home- made reagents. Desalination 239, 139-145.

Yang, J., Wang, G., Ng, T.B., Lin, J., &Ye, X. (2016). Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp. in Response to Metal Ions, Aromatic Compounds, and Nutrients. Frontiers in Microbiology, 6, 1-11.

Yoshida, S., Yonehara, S,. Minami, S., Ha, H., Iwahara, K., Watanabe, T., Honda, Y., & Kuwahara, M. (1996). Production and characterization of ligninolytic enzymes of Bjerkandera adusta grown on wood meal/wheat bran culture and production of these enzymes using a rotary-solid fermenter. Mycoscience 37 (4), 417-425.

Zahra, H., Kurniawan, I., Hakim, A. (2020). The Efficiency of Melanoidin Based-Waste Degradation with Different Biological Methods. Curr. Biochem. 7(2), 52-60.

Zucca, P., Cocco, G., Sollai, F., Sanjust, E. (2015). Fungal laccases as tools for biodegradation of industrial dyes. Biocatalysis 1, 82–108.

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.