Transaminase Enzyme and Liver Histopatological Structure of Mice Facing to Smoke Cigarettes After Administerred with Enhalus acoroides Peel Extract
(1) Biology Education Program, Postgraduate Program University of Pattimura, Ambon Indonesia
(2) Biology Education Program Faculty of Teacher Training and Education University of Pattimura, Ambon Indonesia
(3) Biology Education Program Faculty of Teacher Training and Education University of Pattimura, Ambon Indonesia
Abstract
The objective of this research were aimed to determine the level of transaminase enzymes and the histopathological structure of mice liver exposed to cigarette smoke after treatment of E. acoroides peel extract. Completely randomized design was conducted with 3 doses treatment groups (P1, P2, P3) and a control group (K+, K-) with 3 times repeated. The test was carried out during 14 days for cigarette smoke, and 14 days for seagrass peel extract tretment. The data measured were the levels of SGOT, SGPT and were analyzed using the Anova test. Liver histopathology (necrosis, parenchymatous, hydropic degeneration and normal) were described descriptively. The results showed that the administration of seagrass E. acoroides peel extract had a significant effect on decreasing levels of SGOT, SGPT (p value <0.05) when compared with K+. The P3 treatment had lower levels of SGOT (80.07±3.1 IU/L) and SGPT (88.3±2.1 IU/L). Liver histopathology consist of 34 necrosis cells less than the positive control group (K+) and normal cells were 47, higher than the positive control group (K+). It can be concluded that E. acoroides peel extract had a significant effect on SGOT, SGPT levels in mice exposed to cigarette smoke. Peel extract of E. acoroides 75 mg/kgBW (P3) was an effective dose to reduce the levels of SGOT, SGPT and improve the histopathological structure of mice liver exposed to cigarette smoke. This research is expected to be useful for the community, especially in the coastal areas regarding the benefits of using E. acoroides as a therapeutic agent against free radicals originating from cigarette smoke.
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Alvina, A. (2016). Hepatic enzyme concentrations as indicators of nonalcoholic fatty liver disease. Universa Medicina, 28 (3): 139–145. https://doi.org/10.18051/UnivMed.2009.v28.139-145
Contreras-Zentella, M.L., & Hernández-Muñoz, R. (2016). Is liver enzyme release really associated with cell necrosis induced by oxidant stress. Oxidative Medical Cell Longevity, 2016:3529149. doi: 10.1155/2016/3529149. Epub 2015 Dec 20. PMID: 26798419; PMCID: PMC4699024.
Dewi, C.S.U., Kasitowati, R.D., Siagian, J.A. PShytochemical compounds of Enhalus acoroides from Wanci Island (Wakatobi) and Talango Island (Madura) Indonesia. IOP Conference Series: Earth and Environmental Science, 2017(137).
El-Zayadi, A.R., (2006). Heavy smoking and liver. World Journal of Gastroenterol, 14(12), 6098-101. doi: 10.3748/wjg.v12.i38.6098. PMID: 17036378; PMCID: PMC4088100.
Huang, X.J., Choi, Y.K., Im, H.S., Yarimaga, O., Yoon, E., & Kim, H.S. (2006). Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. Sensors (Basel). 2006;6(7):756–82. PMCID: PMC3894536.
Gono, C.M.P., Ahmadi, P., Hertiani, T., Septiana, E., & Putra, M.Y. (2022). Chianese G. A comprehensive update on the bioactive compounds from Seagrasses. Marine Drugs. 20(406). https://doi.org/10.3390/md20070406
Li, S., Tan, H.Y., Wang, N., Zhang, Z.J., Lao, L., Wong, C.W., & Feng, Y. (2015). The role of oxidative stress and antioxidants in liver diseases. Internasional Journal of Molecular Science, 16(11), 87-124. doi: 10.3390/ijms161125942. PMID: 26540040; PMCID: PMC4661801.
Lala, V., Zubair, M,, Minter, D.A. (2022). Liver function tests. [Updated 2022 Oct 5]. In: Stat Pearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK482489/
Lang, A.L., & Beier, J.I. (2018). Interaction of volatile organic compounds and underlying liver disease: a new paradigm for risk. Biological Chemistry, 25(11), 1237-1248. doi: 10.1515/hsz-2017-0324. PMID: 29924722; PMCID: PMC6181143.
Machado-Junior, P.A., Araújo, N.P.S., Souza, A.B.F., Castro, T.F., & Oliveira, M. (2020). Protective effects of quercetin on livers from nice exposed to long-term cigarette smoke. Biomedical Research International. 2020;15;2020:2196207. doi: 10.1155/2020/2196207. PMID: 33282940; PMCID: PMC7685793.
Mumtazah, D.F., Busman, H., Kanedi, M., & Pratami, G.D. (2021). A modified high-fat diet and its effect on histopathological features of mice liver as an alternative diet for animal model of liver cell damage. Biogenesis: Jurnal Ilmiah Biologi, 9(2), 171–177. doi: https://doi.org/10.24252/bio.v9i2.24081.
Park, S., Kim, J.W., & Yun, H. (2016). Mainstream cigarette smoke accelerates the progression of nonalcoholic steatohepatitis by modulating Kupffer cell-mediated hepatocellular apoptosis in adolescent mice. Toxicology Letters, 2016 (256), 53–63. doi: 10.1016/j.toxlet.2016.05.012.
Pietrangelo, A. (2016). Iron and the liver. Liver International, 2016(36) (Suppl. S1), 116–123. https://doi.org/10.1111/liv.13020
Parthasarath, A., Vanitha, V., Bharathi, N., Jayalakshmi, M., & Mohanasundaram, S. Phytochemycal analysis and invitro antioxidant screening of sea grass-Enhalus acoroides. International Journal of Research in Pharmaceutical Sciences, 8(1), 251-258.
Ramadhani, M.R., Bachri, M.S., & Widyaningsih, W. (2017). Effects of ethanolic extract of arrowroot tubers (maranta arundinacea l.) on the level of MDA, SGPT and SGOT in ethanol induced rats. Indonesian Journal of Medicine and Health. 8(1), 10-18.
Rose, J.J., Wang L, Xu Q., McTiernan CF., Shiva S, Tejero J., & Gladwin, M.T. (2017). Carbon monoxide poisoning: pathogenesis, management, and future directions of therapy. American Journal of Respiratory Criterion Care Medical, 195(5): 596-606. doi: 10.1164/rccm.201606-1275CI
Rutledge, S.M., & Asgharpour, A. (2020). Smoking and liver disease. Gastroenterol Hepatol (N Y), 16(12), 617-625. PMID: 34035697; PMCID: PMC8132692.
Saputro, I.D., Zarasade, L., & Kurniawan, Z. (2022). Elevated serum transaminase (SGOT/SGPT) and sepsis in burn patients in a tertiary hospital, Surabaya, Indonesia. Fol Med Indones, 58(2), 156-161. doi: 10.20473/fmi.v58i2.32865
Supattra, K., Benjaporn, B., Wanida, C., & Somsak, N. (2018). Pharmacognostical and physicochemical studies of Enhalus acoroides (L.F.) Royle (Rhizome). Pharmacognosy Journal. 10(6), 89-94.
Santoso, J., Anwariyah, S., Rumiantin, R.O., Putri, A.P., Ukhty, N.,
& Yoshie-Stark, Y. (2012). Phenol content, antioxidant activity and fibers
profile of four tropical seagrasses from Indonesia. Journal of Coastal Develpopment. 15(2), 189-196.
Simioni, C., Zauli, G., Martelli, A.M., Vitale, M., Sacchetti, G., Gonelli, A., & Neri, L.M. (2018). Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging. Oncotarget, 30;9(24): 17181-17198. doi: 10.18632/oncotarget.24729. PMID: 29682215; PMCID: PMC5908316.
Suparmi, S., Isradji, I., & Fatmawati, D. (2022). The level of SGOT and SGPT after the administration of powdered colorant pigment isolated from Kesumba Kelingâ (Bixa Orellana) seed coats an experimental study in Balb/C Mice. Sains Medika: Jurnal Kedokteran dan Kesehatan, 3(1): 69-77. 2022. doi:http://dx.doi.org/10.26532/sainsmed.v3i1.411.
Setyawati, I., & Aggraeni, R. (2018). Effectiveness of sweet orange peel extract (Citrus sinensis) on the improvement of liver functions of animal trials induced by
cigarette smoke. Journal of Young Pharmacists, 10 (2 Suppl), 132-135.
Shojaie, L., Iorga, A., & Dara, L. Cell Death in Liver Diseases: A Review. International Journal of Molecular Science, 2020(21), 86-92. doi:10.3390/ijms21249682
Wang, L., Wang, Y., Chen, J., & Li, M. (2022). A review of toxicity mechanism studies of electronic cigarettes on respiratory systems. Internasional Journal of Molecular Science. 23(9), 50-30; https://doi.org/10.3390/ijms23095030
Widiastuti, E.L., Rima, K., & Busman, H. Anticancer Potency of Seagrass (Enhalus acoroides) Methanol Extract in the HeLa Cervical Cancer Cell
Culture. Advances in Engineering Research, 2021(202), 38-42.
Widiastut, E.L, Khairani, I.A., Cristianto, Y., Nurcahyani. E., Nurcahyani, N., Satria, H. (2019). Effect of Jeruju leaves and lamun methanolic extract also
taurine on blood and liver histophatology of male mice
induced by benzo(α)piren. Jurnal Lembaga Penelitian dan Pengabdian Masyarakat Universitas Lampung 2019.
Zong, D., Liu, X., Li, J., Ouyang, R., & Chen, P. (2019). The role of cigarette smoke-induced epigenetic alterations in inflammation. Epigenetics & Chromatin, 12(1): doi: 10.1186/s13072-019-0311-8
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