Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei

Imam Rosadi, Mukh Syaifudin, Dewi Elfidasari

Abstract


Indonesia had a high number of malaria cases where the treatments are only perceived by 10% of patients in health facilities. The resistance of Plasmodium sp. to drugs causes the difficulty of controlling malaria. The use of irradiation to produce malaria vaccine material is being explored until nowadays. In previously study that dose rate of gamma irradiation delivered to parasite was at the low dose rate (380 Gy/hour) which produced several doses that not be able to attenuate the parasite. This research aimed to increase dose rate (740 Gy/hour) and to determine the dose irradiation effect of 150-250 Gy to the protein profile, erythrocyte and leukocyte numbers in mouse blood infected by P. berghei erythrocytic stage. Results showed that there was an influence of dose rate of 740 Gy/hour for doses of 150-250 Gy in treated mice and no difference in erythrocyte, leucocyte and body weight with negative control. Dose of 150 Gy was the optimal dose to attenuate parasites, whereas doses more than 150 Gy effectively killed the parasites. The changes of protein profile marked by the appearance of higher number of bands at molecular weight of 30-37 kDa that predicted as merozoite surface protein of P. berghei and/or antibodies elicited in mouse.

How to Cite

Rosadi, I., Syaifudin, M., & Elfidasari, D. (2016). Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei. Biosaintifika: Journal of Biology & Biology Education, 8(2), 155-164


Keywords


malaria vaccine; irradiation; Plasmodium berghei; leucocyte and erythrocyte; protein profiles

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References


Akuba, B. O. & Amlabu, E. (2013). Purification and biochemical characterization of serine proteases from Plasmodium berghei NK-65. International Journal of Science, 2(2), 104-112.

Asih, P. B. S., Syahrani, L., Rozi, I. E.P., Pratama, N. R., Marantina, S. S., Mangunwardoyo, W., Hawley, W., Laihad, F., Shinta., Arsyad, D.S., Sukowati, S., Lobo, N.F., Syafruddin, D. (2012). Existence of the rdl mutant alleles among the anopheles malaria vector in Indonesia. Malaria Journal, 11(1), 1.

Autino, B., Corbett, Y., Castelli, F. & Taramelli, D., (2012). Pathogenesis of malaria in tissues and blood. Mediterranean Journal of Hematology and Infectious Diseases, 4(1), 1-12.

Autino, B., Noris, A., Russo, R., Castelli, F. (2012). Epidemiology of malaria in endemic areas. Mediterranean Journal of Hematology and Infectious Diseases, 4(1), 2012060.

Bagai, U., Pawar, A., Kumar, V. (2010). Antibody responses to 43 and 48 kDa antigens of blood-stage Plasmodium berghei in Balb/c mice. Journal of Parasitic Diseases, 34(2), 6874.

Basir, R., Fazalul-Rahiman, S.S., Hasballah, K., Chong, W.C., Talib, H., Yam, M.F., Jabbarzare, M., Tie, T.H., Othman, F., Moklas, M.A.M., Abdullah, W.O. (2015). Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria. Iranian Journal Parasit, 7(4), 62-74.

Cortes, G. T., Winograd, E., Wiser, M. F. (2003). Characterization of proteins localized to a subcellular compartment associated with an alternate secretory pathway of the malaria parasite. Molecular and Biochemical Parasitology, 129(2), 127-135.

Crompton, P. D., Pierce, S. K., Miller, L. H. (2010). Advances and challenges in malaria vaccine development. Journal of Clinical Investigation, 120(12), 4168-4178.

Darlina, & Tetriana, D. (2006). Pengaruh irradiasi gamma pada Plasmodium berghei terhadap daya tahan mencit. Retrieved from http://www.batan.go.id/NHC/dokumen/devita4.pdf.

Delves, M., Plouffe, D., Scheurer, C., Meister, S., Wittlin, S., Winzeler, E.A., Sinden, R.E. and Leroy, D., (2012). The activities of current antimalarial drugs on the life cycle stages of Plasmodium: a comparative study with human and rodent parasites. PLOS Medicine, 9(2), 165-176.

Demicheli, M. C., Reis, B. S., Goes, A. M., De Andrade, A. S. R. (2006). Paracoccidioides brasiliensis: attenuation of yeast cells by gamma irradiation. Mycoses, 49(3),184-189.

Hill, A. V. (2011). Vaccines against malaria. Journal of Biological Sciences, 366(1579), 2806-2814.

Hoffman, S.L., Goh, L.M.L., Luke, T.C., Schneider, I., Le, T.P., Doolan, D.L., Sacci, J., Vega, Pdl., Dowler, M., Paul, C., Gordon, D.M., Stoute, J.A., Church, L.W.P., Sedegah, M., Heppner, D.G., Ballou, W.R., Richie, T.L. (2002). Protection of Humans against Malaria by Immunization with Radiation-Attenuated Plasmodium falciparumSporozoites. Journal of Infectious Diseases, 185(8), 1155-1164.

Jobe, O., Lumsden, J., Mueller, A.K., Williams, J., SilvaRivera, J., Kappe, S.H.I., Schwenk, R.J., Matuschewski, K., Krzych, U. (2007). Genetically-attenuated Plasmodium berghei liver-stages induce sterile protracted protection that is mediated by MHC class I dependent IFN-? producing CD8+ T cells. Journal of Infectious Diseases, 196(4), 599-607.

Krishna, S., Bharti, P.K., Chandel, H.S., Ahmad, A., Kumar, R., Singh, P.P., Singh, M.P. Singh, N., (2015). Detection of mixed infections with Plasmodium spp. by PCR, India, 2014. Emerging Infectious Diseases, 21(10), 1853.

Lamikanra, A.A., Brown, D., Potocnik, A., Casals-Pascual, C., Langhorne, J., Roberts, D. J. (2007). Malarial anemia: of mice and men. Blood, 110(1), 18-28.

Marti, M., Good, R.T., Rug, M., Knuepfer, E., Cowman, A.F. (2004). Targeting malaria virulence and remodeling proteins to the host erythrocyte. Science, 306(5703), 1930-1933.

Ministry of Health Republic Indonesia (KEMENKES). (2011). Epidemiologi Malaria di Indonesia. Jakarta : Bakti Husada.

Mlambo, G. & Kumar, N. (2008). Transgenic rodent Plasmodium berghei parasites as tools for assessment of functional immunogenicity and optimization of human malaria vaccines. Eukaryotic Cell, 7(11), 1875-1879.

Spiering, M. J. (2015). Primer on the Immune System : current reviews. J Alcohol Res, 37(2), 171-175.

Syaifudin, M. (2014). Peranan Faktor Imun dan Profil Protein dalam Penelitian dan Pengembangan Vaksin Malaria Iradiasi. Biosaintifika: Journal Biology & Biology Education, 6(1), 8-17.

Syaifudin, M., Nurhayati, S., Tetriana, D. (2008). Pengembangan vaksin malaria dengan radiasi pengion. Dalam: Prosiding Seminar Nasional Sains dan Teknologi-II. Lampung, 17-18 November 2008. Lampung: SemnasSaintek-II.

Syaifudin, M., Tetriana, D., Darlina, Nurhayati, S. (2013). Studies on the protein profiles of gamma ray induced blood stage of Plasmodium berghei for developing candidate of malaria vaccine. International Journal of Engineering Research and Applications, 3(1), 314-319.

Tetriana, D., Darlina., Armanu., Syaifudin, M. (2008). Pengaruh radiasi gamma terhadap profil protein Plasmodium berghei stadium eritrositik. Dalam: Prosiding Seminar Nasional Keselamatan, Kesehatan dan Lingkungan IV dan International Seminar on Occupational Health and Safety I. Buku 1. Depok, 27 Agustus 2008. Depok: Int on Occup Health and Safe I. pp. 282-292.

Tewari, R., Ogun, S.A., Gunaratne, R.S., Crisanti, A., Holder, A.A. (2004). Disruption of Plasmodium berghei merozoite surface protein 7 gene modulates parasite growth in vivo. Blood, 105(1), 394-396.

Wickham, M. E., Culvenor, J. G., Cowman, A. F. (2003). Selective inhibition of a two-step egress of malaria parasites from the host erythrocyte. Journal of Biological Chemistry, 278(39), 37658-37663.

Yahya, M.F.Z.R. & Kadir, R.A. (2013). Palm oil supplementation prevents further tyrosine dephosphorylation of erythrocytes 30-kda protein during Plasmodium berghei infection. Journal of Pharma and Bio Sciences, 3, 131-137.

Zuzarte-Luis, V., Mota, M. M., Vigrio, A. M. (2014). Malaria infections: what and how can mice teach us. Journal of Immunological Methods, 410, 113-122.




DOI: https://doi.org/10.15294/biosaintifika.v8i2.6341

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