Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw

Ateng Supriyatna, Ukit Ukit

Abstract


A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 0C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. Bacillus sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24th, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (Hermetia illucens) have competencein organic waste degradation, because in Black Soldier Flays Larvaes gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially Bacillus sp,

How to Cite

Supriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw. Biosaintifika: Journal of Biology & Biology Education, 8(3), 314-320.


Keywords


The enzyme activity; cellulolytic bacteria; Larva Black Soldier; Isolation and Screening

Full Text:

PDF

References


Apun, K., Jong, B. C., & Salleh, M. A. (2000). Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste. The Journal of general and applied microbiology, 46(5), 263-267.

Baharuddin, R., Hock, A., Aziz, R., & Shah, H. (2010). (2010). Isolation and characterization of thermophilic cellulase-producing bacteria from empty fruit bunches-palm oil mill effluent compost. American Journal of Applied Sciences, 7(1), 56-62.

Benson, H. J. (2002). Microbiogical Applications Laboratory Manual in General Microbiology 8th Edition. NewYork: Mc Graw Hill.

Demirkan, E., Dincbas, S., Sevinc, N., & Ertan, F. (2011). Immobilization of B. amyloliquefaciens ?-amylase and comparison of some of its enzymatic properties with the free form. Romanian Biotechnological Letters, 16(6), 6690-6701.

Denyer, S. P., Hodges, N. A. & Gorman, S. P. (2004). Pharmaceutical Microbiology. Australia: Blackwell Publishing.

Drake, D., Nader, G., & Forero, L. (2002). Feeding rice straw to cattle. Canada: UCANR Publications.

Hong, S.T., Chang, G., dan Ho, K. (2007). Characterization of facultative thermophilic microbial community of composts by ARDRA. Taiwania, 52(2), 117-126.

Howard, R. L., Abotsi, E., Van Rensburg, E. J., & Howard, S. (2003). Lignocellulose biotechnology: issues of bioconversion and enzyme production. African Journal of Biotechnology, 2(12), 602-619.

Johnvesly, B., & Naik, G. R. (2001). Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium. Process biochemistry, 37(2), 139-144.

Kim, Y. K., Lee, S. C., Cho, Y. Y., Oh, H. J., & Ko, Y. H. (2012). Isolation of cellulolytic Bacillus subtilis strains from agricultural environments. ISRN microbiology, 2012.

Ma, J., Zhang, Z., Wang, B., Kong, X., Wang, Y., Cao, S., & Feng, Y. (2006). Overexpression and characterization of a lipase from Bacillus subtilis. Protein expression and purification, 45(1), 22-29.

Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analit Chem, 31(3), 426-428

Ohkuma, M. (2003). Termite symbiotic systems: efficient bio-recycling of lignocellulose. Applied microbiology and biotechnology, 61(1), 1-9.

Pelczar, M. J. & Chan, E. C. S. (1988). Dasar-dasar Mikrobiologi. Jakarta: UI Press.

Pouderoyen, G, V., Eggert, T., Jaeger, K. E., & Dijkstra, B. W. (2001). The crystal structure of Bacillus subtili lipase: a minimal ?/? hydrolase fold enzyme. Journal of molecular biology, 309(1), 215-226.

Shaheb, M, S, A., Youris, M, A, M., Fezayen, F, F., Eldein, M, A, N. (2010). Production Of Cellulase In Low-Cost Medium By Bacillus subtilis KO Strain. World ApplSci J, 8(1), 35-42.

Sheppard, D. C., Newton, G. L., Thompson, S. A., & Savage, S. (1994). A value added manure management system using the black soldier fly. Bioresource Technology, 50(3), 275-279.

Singh, M, J., Surav, K., Srivastava, N., & Kannabrian, K. (2010). Lipase Production By Bacillus Subtilis OCR-4 In Solid Fermentation Using Ground Nut Oil Cakes As Substrate. Current Research Journal of Biological Sciences, 2(4), 241-245.

Wannapeera, J., Worasuwannarak, N., & Pipatmanomai, S. (2008) Product Yields and Characteristic Of Rice Husk, rice straw and corncob during fast pyrolysis in a drop-tube/fixed-bed reactor. Technol, 30(3), 393-404.

Yin, L, J., Lin, H, H., & Xiao, Z, R. (2010). Purification and Characterization Of A Cellulase FromBacillus subtilis YJ1. Journal of Marine Science and Technology, 18(3), 466-471

Yu, G., Cheng, P., Chen, Y., Li, Y., Yang, Z., Chen, Y., Tomberlin, J, K., (2011). Inoculating Polutry Manure With Companion Bacteria Influences Growth and Development of Balck Soldier Fly (Diptera: Strtiomyidae) Larvae. Entomology, 40(1), 30-35.




DOI: https://doi.org/10.15294/biosaintifika.v8i3.6762

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.