Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa

Sri Puji Astuti Wahyuningsih, Nadyatul Ilma Indah Savira, Win Darmanto

Abstract


Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains ?-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.

How to Cite

Wahyuningsih, S. P. A., Savira, N. I. I, & Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology & Biology Education, 8(3), 308-313.


Keywords


Polysaccharide krestin; phagocytic activity; phagocytic capacity

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References


Abbas, A. K., Litchmann, A. H. H. & Pillai, S. (2012). Celluler and Molecular Immunology, 7th Edition. Philadelphia: WB Saunders Company.

Baratawidjaya, K. G. (2006). Imunologi Dasar, Edisi 7. Jakarta: Balai Penerbit FK UI.

Chan, G. C. F., Chan, W. K., & Sze, D. M. Y. (2009). The effects of ?-glucan on human immune and cancer cells. Journal of hematology & oncology, 2(1), 1.

Cui, J., & Chisti, Y. (2003). Polysaccharopeptides of Coriolus versicolor: physiological activity, uses, and production. Biotechnology advances, 21(2), 109-122.

DElios, M. M., Benagiano, M., Della Bella, C., & Amedei, A. (2011). T-cell response to bacterial agents. The Journal of Infection in Developing Countries, 5(09), 640-645.

Handojo, I. (2003). Pengantar Imunoasai Dasar. Surabaya: Airlangga University Press.

Hong, F., Yan J., Baran J. T., Allendorf D. J., Hansen R. D., & Ostroff G. R. (2004). Mechanism by which orally administered ?-1, 3-glucans enhance the tumoricidal activity of antitumor monoclonal antibodies in murine tumor models. The Journal of Immunology, 173(2), 797-806.

Jang, S. A., Park, S. K., Lim, J. D., Kang, S. C., Yang, K. H., Pyo, S. K., & Sohn, E. H. (2009). The comparative Immunomodulatory effects of ?-glucans from yeast, bacteria, and mushroom on the function of macrophages. Preventive Nutrition and Food Science, 14(2), 102-108.

Koendhori, E. B. (2008). Peran Ethanol Extract Propolis terhadap Produksi Interferon ?, Interleukin 10, dan Transforming Drowth Factor ?1 serta Kerusakan Jaringan Paru pada Mencit yang Diinfeksi Dengan Myvobacterium Tuberculosis. Disertasi. Surabaya: Program Pasca Sarjana. Universitas Airlangga.

Kusmardi, S. K., & Enif, E. T. (2007). Efek Imunomodulator Ekstrak Daun Ketepeng Cina (Cassia Alata L.) terhadap Aktivitas dan Kapasitas Fagositosis Makrofag. Makara Kesehatan, 11(2), 50-53

LeBlanc, B. W., Albina, J. E., & Reichner, J. S. (2006). The effect of PGG-?-glucan on neutrophil chemotaxis in vivo. Journal of Leukocyte Biology, 79(4), 667-675.

Ridconi, A., Hariyo S., & Uripno A. (2011). Midazolam Intravena Dosis Rendah Tidak Mempengaruhi Nitric Oxide Intraperitoneal Mencit Balb/C Yang Terpapar Lipopolisakarida. Jurnal Anestesiologi Indonesia, (2), 84-94

Rifai, M. (2009). Signal Transduksi dan Sistem Pertahanan Tubuh. Malang: Galaxy Science.

Todar, K. (2004). Textbook of Bacteriology: Pseudomonas aeruginosa. USA: University of Wisconsin-Madison Department of Bacteriology.

Wahyuningsih, S., & Win, D. (2010). Uji Toksisitas Akut Polisakarida Krestin dari Ekstrak dan Miselium Jamur Coriolus versicolor: Upaya Menggali Potensi Bahan Hayati sebagai Imunomodulator Respon Imun terhadap Mycobacterium tuberculosis. Laporan Penelitian. Surabaya: Universitas Airlangga.

Wahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysac-charide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.




DOI: https://doi.org/10.15294/biosaintifika.v8i3.6957

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