Resistance Inheritance of Plutellaxylostella Population to Residual of Emamectin Benzoat

Udi Tarwotjo, Rully Rahardian

Abstract


Excessive use of insecticides drives the increasing ability of pests to become resistant. The objectives of this research were to study the susceptibility and the resistance inheritance of the eleven population of P. xylostella to emamectin benzoate. The leaf-dip bioassay was applied to determine the sensitivity of P. xylostella to emamectin benzoate. The offspring of backcrossed F2 were tested whether the resistance was controlled by monogenic. The results showed that the LC50 of the Selo population was 53.42 ppb, and the Puasan population was 212.13 ppb. The genetic analysis showed that the backcrosseddegree of dominance (D) was less than 1. It was indicated that the P. xylostella resistance to emamectin benzoate was recessive. The value of LC50 of the backcrossed F1? x ?S (177.99 ppb) and its reciprocals x ?R (F1) (201.69 ppb) were not significantly different with the value of LC50 resistance population. This suggests that the nature of P. xylostella resistance to emamectin benzoate was controlled by monogenic.The result of the study would be beneficial for developing strategy to maintain susceptible population using refugee plant during lack of their host.


Keywords


resistance; Plutellaxylostella; emamectinbenzoate; recessive; monogenic

Full Text:

PDF

References


Abate, T., van Huis, A., & Ampofo, J. K. O. (2000). Pest management strategies in traditional agriculture: an African perspective. Annual review of entomology, 45(1), 631-659.

Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol, 18(2), 265-267.

Attique, M. N. R., Khaliq, A., & Sayyed, A. H. (2006). Could resistance to insecticides in Plutella xylostella (Lep., Plutellidae) be overcome by insecticide mixtures?. Journal of Applied Entomology, 130(2), 122-127.

Bailey, W. D., Brownie, C., Bacheler, J. S., Gould, F., Kennedy, G. G., Sorenson, C. E., & Roe, R. M. (2001). Species diagnosis and Bacillus thuringiensis resistance monitoring of Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae) field strains from the southern United States using feeding disruption bioassays. Journal of Economic Entomology, 94(1), 76-85.

Brewer, M. J., & Trumble, J. T. (1991). Inheritance and fitness consequences of resistance to fenvalerate in Spodoptera exigua (Lepidoptera: Noctuidae). Journal of Economic Entomology, 84(6), 1638-1644.

Busvine, J. R. (2002). A Critical Review of the Techniques for Testing Insecticides. London: Common-wealth Agriculture Bureaux.

Bynum, E. D., & Archer, T. L. (2000). Identifying insecticide-resistant greenbugs (Homoptera: Aphididae) with diagnostic assay tests. Journal of Economic Entomology, 93(4), 1286-1292.

Carrire, Y., Ellers-Kirk, C., Liu, Y. B., Sims, M. A., Patin, A. L., Dennehy, T. J., & Tabashnik, B. E. (2001). Fitness costs and maternal effects associated with resistance to transgenic cotton in the pink bollworm (Lepidoptera: Gelechiidae). Journal of Economic Entomology, 94(6), 1571-1576.

Cummings, M. R., & Klugs, W. S. (1997). Conceps of Genetics. New Jersey: Prentice Hall Inc.

Georghiou, G. P., & Taylor, C. E. (1986). Factors Influencing the Evolution of Resistance, pp. Pest Population (eds), Pesticides Resistance: Strategies and Tactics for Management.National Academy Press Washington, D.C.

Groeters, F. R., & Tabashnik, B. E. (2000). Roles of selection intensity, major genes, and minor genes in evolution of insecticide resistance. Journal of Economic Entomology, 93(6), 1580-1587.

Harwanto. (2014). Pengaruh Ekstrak Limbah Daun Tembakau Madura terhadap Aktivitas Makan Larva Spodoptera exigua. Biosaintifika: Journal of Biology & Biology Education, 4(1), 1-4.

Hillocks, R. J. (2012). Farming with fewer pesticides: EU pesticide review and resulting challenges for UK agriculture. Crop Protection, 31(1), 85-93.

Huang, F., & McGaughey, W. H. (1999). Inheritance of resistance to Bacillus thuringiensis toxin (Dipel ES) in the European corn borer. Science, 284(5416), 965-967.

Khodijah. (2014). Kelimpahan Serangga Predator kutu daun Aphis gossypii di Sentra Tanaman Sayuran di Sumatera Selatan. Biosaintifika: Journal of Biology & Biology Education, 6(2), 52-53.

Listyaningrum, W., Trisyono, Y. A., & Aziz, P. (2003). Pewarisan Sifat resistensi terhadap deltamethrin pada Plutella xylostella. L. J. Perlin. Tan. Indon, 9(1), 135-140

Maron, P. C. R. G., Siegfried, B. D., Spencer, T., & Hutchison, W. D. (2000). Development of diagnostic concentrations for monitoring Bacillus thuringiensis resistance in European corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology, 93(3), 925-930.

Mascarenhas, R. N., & Boethel, D. J. (2000). Development of diagnostic concentrations for insecticide resistance monitoring in soybean looper (Lepidoptera: Noctuidae) larvae using an artificial diet overlay bioassay. Journal of Economic Entomology, 93(3), 897-904.

Moekasan. (2004). Percampuran Spodoptera Nuclear Polyhidrosis Virus dengan Insektisida Kimia untuk Mortalitas Larva Spodoptera exygua Hubn. Di Laboratorium. J. Hort. 14(3), 178-187.

Moulton, J. K., Pepper, D. A., Jansson, R. K., & Dennehy, T. J. (2002). Pro-active management of beet armyworm (Lepidoptera: Noctuidae) resistance to tebufenozide and methoxyfenozide: baseline monitoring, risk assessment, and isolation of resistance. Journal of Economic Entomology, 95(2), 414-424.

Nuryanti, N. S. P. (2001). Sensitivity Some Plutellaxylostella populations in Central Java and Yogyakarta to deltamethrin. Thesis. Yogyakarta: Graduate School, Faculty of Agriculture, University of Gadjah Mada.

Prez, C. J., Alvarado, P., Narvez, C., Miranda, F., Hernndez, L., Vanegas, H., Hruska, A., & Shelton, A. M. (2000). Assessment of Insecticide Resistance in Five Insect Pests Attacking Field and Vegetable Crops in Nicaragua. Journal of Economic Entomology, 93(6), 1779-1787.

Sato, M. E., Miyata, T., Da Silva, M., Raga, A., & De Souza Filho, M. F. (2004). Selections for fenpyroximate resistance and susceptibility, and inheritance, cross-resistance and stability of fenpyroximate resistance in Tetranychus urticae Koch (Acari: Tetranychidae). Applied Entomology and Zoology, 39(2), 293-302.

Setiawati, W. (2000). Pengendalian hama kubis Plutella xylostella L. dan Crocidolomia binotalis Zell. dengan Spinosad 25 SC serta pengaruhnya terhadap parasitoid Diadegma semiclausum Hellen. J. Hort, 10(1), 30-39.

SShelton, A. M., Sances, F. V., Hawley, J., Tang, J. D., Boune, M., Jungers, D., Collins, H. L., & Farias, J. (2000). Assessment of Insecticide Resistance. After the Outbreak of Diamondback Moth (Lepidoptera: Plutellidae) in California in 1997. Journal of Economic Entomology, 93(3), 931-936.

Siegfried, B. D., Spencer, T., & Nearman, J. (2000). Baseline susceptibility of the corn earworm (Lepidoptera: Noctuidae) to the Cry1Ab toxin from Bacillus thuringiensis. Journal of Economic Entomology, 93(4), 1265-1268.

Tabashnik, B. E., Schwartz, J. M., Finson, N., & Johnson, M. W. (1992). Inheritance of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology, 85(4), 1046-1055.

Tabashnik, B. E., Liu, Y. B., Dennehy, T. J., Sims, M. A., Sisterson, M. S., Biggs, R. W., & Carrire, Y. (2002). Inheritance of resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Lepidoptera: Gelechiidae). Journal of Economic Entomology, 95(5), 1018-1026.

Trisyono. (2002). Ecdyson Agonists New Insecticides with Novel Mode of Action. J. Perlind. Tan. Indon. 8, 75-85.

Ware, G. W. (2004). Fundamental of Pesticide. 2nd Edition. Thomson Publication Fresno

Wearing, C. H., Lo, P., & Walker, J. T. S. (2000). Concern about Managing Organophosphat-Resistant Populations of Leafrollers in New Zelland with Tebufenozide. Pestic.Sci. 9, 8-9.

Winarto, L., & Nazir. (2004). Teknologi Pengendalian Hama Plutella xylostella dengan Insektisida dan Agensia Hayati pada Kubis di Kabupaten Karo. Jurnal Pengkajian dan Pengembangan Teknologi Pertanian, 7(1), 27-33.

Woodford, J., Moll, A. J., Palalo, K., & Suparta, L. (2015). The Use of Agrochemicals on Tomato and Cabbage Crops in West Java. Bandung: Balittan Lembang.

Zhao, J. Z., Bishop, B. A., & Grafius, E. J. (2000). Inheritance and synergism of resistance to imidacloprid in the Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 93(5), 1508-1514.




DOI: https://doi.org/10.15294/biosaintifika.v9i1.8048

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.