Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

Lenni Fitri, Anja Meryandini, Dyah Iswantini, Yulin Lestari

Abstract


Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 g/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.


Keywords


antiobesity; endophyte; pancreatic lipase inhibitor; Streptomyces sp. AEBg12

Full Text:

PDF

References


Adnyana, I. K., Sukandar, E. Y., Yuniarto, A., & Finna, S. (2014). Anti-obesity effect of the pomegaranate leaves ethanol extract (Punicagranatum L) in high-fat diet induced mice. International Journal of Pharmacy and Pharmaceutical Sciences, 6(4), 626-631.

Ahn, J. H., Liu, Q., Lee, C., Ahn M. J., Yoo, H. S., Hwang, B. Y., & Lee M. K. (2012). A new pancreatic lipase inhibitor from Broussonetia kanzinoki. Bioorganic & Medicinal Chemistry Letters, 22(8), 2760-2763

Arifianti, L., Oktarina, R. D., & Kusumawati, I. (2014). Pengaruh jenis pelarut pengektraksi terhadap kadar sinensetin dalam ekstrak daun Orthosiphon stamineus Benth. E-Journal Planta Husada, 2(1), 1-4.

Ayini, U., Harnina, B. S., & Dewi, T. C. (2014). Efek antibakteri ekstrak daun mimba (Azadirachta indica A. Juss) terhadap bakteri Vibrio algynoliticus secara in vitro. Biosaintifika: Journal of Biology & Biology Education, 6(1), 67-75.

Azis, T., Febrizky, S., & Mario, A. D. (2014). Pengaruh jenis pelarut terhadap persen yield alkaloid dari daun salam india (Murraya koenigii). Teknik Kimia, 2(20), 1-6.

Dzomba, P., & Musekiwa, C. (2014). Anti-obesity and antioxidant activity of dietary flavonoids from Dioscorea steriscus tubers. Journal of Coastal Life Medicine, 2(6), 465-470.

Etoundi, C. B., Kuat, D., Ngondi, J. L., & Oben, J. (2010). Anti-amylase, anti-lipase and antioxidant effects of aqueous extracts of some Cameroonian spices. Journal of Natural Products, 3, 165-171.

Ferreira, T., & Rasband, W. (2012). ImageJ user guide IJ 1.46r. National Institute of Health [internet]. Diambil dari http://www.imagej.nih.gov/ij/docs/guide. diakses 12 Mei, 2016.

Hadrich, F., Cher, S., Gargouri, Y. T., & Adel, S. (2014). Antioxidant and lipase inhibitory activities and essential oil composition of pomegranate peel extracts. Journal of Oleo Science, 63(5), 515-525.

Iswantini, D., Silitonga, R. F., Martatilofa, E., & Darusman, L. K. (2011). Zingiber cassumunar, Guazomaulmifolia, and Murraya paniculata extracts as anti-obesity: in vitro inhibitory effect on pancreatic lipase activity. Hayati, 18(1), 6-10.

Kekuda, T. R. P., & Onkarappa, R. (2014). Antioxidant, antihelmintic and enzyme inhibitory potential of Streptomyces variabilis Strain PO-178 Isolated form western ghat soil, Agumbe, Karnataka, India. Journal of Biological Scientific Opinion, 2(2), 170-176.

Kekuda, T. R. P., Shobha, K. S., & Onkarappa, R. (2011). Pancreatic lipase inhibitory and cytotoxic potential of a Streptomyces species isolated from western ghat soil, Agumbe, Karnataka, India. International Journal of Pharmaceutical and Biological Archives, 2(3), 932-937.

Kumar, S., & Alagawadi, K. R. (2013). Anti-obesity effects of galangin, a pancreatic lipase inhibitor in cafeteria diet fed female rats. Pharmaceutical Biology, 51(5), 607-613

Kusari, S., Verma, V. C., Lamshoeft, M., & Spiteller, M. (2012). An endophytic fungus from Azadirachta indica A. Juss.that produces azadirachtin. World Journal of Microbiology and Biotechnology, 28(3),1287-1294.

Lee, E. M., Lee, S. S., Chung, B. Y., Cho, J. Y., Lee, I. C., Ahn, S. R., Jang, S. J., & Kim, T. H. (2010). Pancreatic lipase inhibition by C-Glycosidic flavones isolated from Eremochloa ophiuroides. Molecules, 15(11), 8251-8259.

Markham K. R. (1988). Cara mengidentifikasi flavonoid. Bandung: Penerbit ITB.

Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E., & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Journal of Medicinal Plants Research, 45(05), 31-34.

Mopuri, R., & Meriga, B. (2014). Anti-Lipase and anti-obesity activities of Terminalia paniculata bark in high calorie diet-induced obese rats. Global Journal of Pharmacology, 8(1), 114-119.

Muaja, A. D., Koleangan, H. S. J., & Runtuwene, M. R. J. (2013). Uji toksisitas dengan metode BSLT dan analisis kandungan fitokimia ekstrak daun soyogik (Saurauia bracteosa DC) dengan metode soxhletasi. Jurnal Mipa Unsrat, 2(2), 115-118.

Pradono, D. I., Darusman, L. K.,& Susanti, A. (2011). Inhibisi lipase pankreas secara in vitro oleh ekstrak air dan etanol daun asam jawa (Tamarindus indica) dan rimpang kunci pepet (Kaempferiae rotundae). Jurnal Natur Indonesia, 13(2), 146-154.

Pujiyanto, S. (2012). Kajian inhibitor ?-glukosidase aktinomiset endofit asal brotowali (Tinospora crispa). Disertasi. Bogor: Program Pasca Sarjana. Institut Pertanian Bogor.

Shin, J. E., Han, M. J., & Kim, D. H. (2003). 3-Methylethergalangin isolated from Alpinia officinarum inhibits pancreatic lipase. Biological & Pharmaceutical Bulletin, 26(6), 854-857.

Septiana, A. T., & Asnani, A. (2012). Kajian sifat fisikokimia ekstrak rumput laut coklat Sargassum duplicatum menggunakan berbagai pelarut dan metode ekstraksi. Agrointek, 6(1), 22-28.

Tan, R. X., & Zou, W. X. (2001). Endophytes: a rich source of functional metabolites. Natural Products Reports, 18(4), 448-459.

Tantithanagorngul, W., Sujitwanit, A., Piluk, J., Tolieng, V., Petsom, A., Sangvanich, P., Palaga, T., Puthong, S., Thamchaipenet, A., & Pinphanichakarn, P. (2011). Screening for brine shrimp larvicidal activity of Streptomyces sp. isolated from soil and anti-tumor activity of the active isolates. Australian Journal of Basic and Applied Sciences, 5(7), 15-22.

Veeresham, C., Kumar, M. R., Sowjanya, D., Kokate, C. K., & Apte, S. S. (1998). Production of azadirachtin from callus cultures of Azadirachta indica. Fitoterapia, 69(5), 423-424.

Yuhernita, J. (2011). Analisis senyawa metabolit sekunder dari ekstrak metanol daun surian yang berpotensi sebagai antioksidan. Makara Sains, 15(1), 48-52.

Yuniarto, A., Purwani, H., Juanda, D., Setiawan, F., & Kurnia, I. (2015). Kumis kucing (Orthosiphon stamineus [benth.]) leaves ethanol extract as anti-obesity agent in high-fat diet-induced obese mice. Asian Journal of Pharmaceutical and Clinical Research, 8(6), 234-236.




DOI: https://doi.org/10.15294/biosaintifika.v9i2.8907

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.