Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus

Awan Purnawan, Yopi Yopi, Tun Tedja Irawadi

Abstract


The increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan.


Keywords


Palm Kernel Cake (PKC); Streptomyces cyaenus; mannanase enzyme; mannooligosaccharide

Full Text:

PDF

References


Agblevor, F. A., Murden, A., & Hames, B. R. (2004). Improved method of analysis of biomass sugars using high-performance liquid chromatography. Biotechnology letters, 26(15), 1207-1211.

Cerqueira, M. A., Bourbon, A. I., Pinheiro, A. C., Martins, J. T., Souza, B. W. S., Teixeira, J. A., & Vicente, A. A. (2011). Galactomannans use in the development of edible films/coatings for food applications. Trends in Food Science & Technology, 22(12), 662-671.

Cerver, J. M., Skovgaard, P. A., Felby, C., Srensen, H. R., & Jrgensen, H. (2010). Enzymatic hydrolysis and fermentation of palm kernel press cake for production of bioethanol. Enzyme and microbial technology, 46(3), 177-184.

Chapla, D., Pandit, P., & Shah, A. (2012). Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. Bioresource Technology, 115, 215-221.

Dhawan, S., & Kaur, J. (2007). Microbial mannanases: an overview of production and applications. Critical reviews in biotechnology, 27(4), 197-216.

Hgglund, P. (2002). Mannan-hydrolysis by hemicellulases Enzyme-polysaccharide interaction of a modular ?-mannanase. Desertation. Sweden: Department of Biochemistry Lund University.

Hilge, M., Gloor, S. M., Rypniewski, W., Sauer, O., Heightman, T. D., Zimmermann, W., ... & Piontek, K. (1998). High-resolution native and complex structures of thermostable ?-mannanase from Thermomonospora fuscasubstrate specificity in glycosyl hydrolase family 5. Structure, 6(11), 1433-1444.

Jrgensen, H., Sanadi, A. R., Felby, C., Lange, N. E. K., Fischer, M., & Ernst, S. (2010). Production of ethanol and feed by high dry matter hydrolysis and fermentation of palm kernel press cake. Applied biochemistry and biotechnology, 161(1-8), 318-332.

Juanssilfero, A. B., Djohan, A. C., Purnawan, A., & Yopi, Y. (2015). Bioethanol Production from Indica IR. 64 Rice Straw Biomass by Direct Saccharification and Fermentation. International Journal on Advanced Science, Engineering and Information Technology, 5(1), 1-5.

Miller, G. L. (1959). Use oi Dinitrosalicylic Acid Reagent tor Determination oi Reducing Sugar. Analytical chemistry, 31(3), 426-428.

Moreira, L. R. S. (2008). An overview of mannan structure and mannan-degrading enzyme systems. Applied microbiology and biotechnology, 79(2), 165.

Muchtadi, D. (1996). Gizi dan kesehatan :Oligosakarida yang menyehatkan. Bogor: Departemen Teknologi Ilmu Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor.

Nakakuki, T. (2002). Present status and future of functional poligosaccharide development in Japan. Pure Appl. Chem, 74(7), 12451251.

Sachslehner, A., Foidl, G., Foidl, N., Gbitz, G., & Haltrich, D. (2000). Hydrolysis of isolated coffee mannan and coffee extract by mannanases of Sclerotium rolfsii. Journal of Biotechnology, 80(2), 127-134.

Sachslehner, A., & Haltrich, D. (1999). Purification and some properties of a thermostable acidic endo-?-1, 4-D-mannanase from Sclerotium (Athelia) rolfsii. FEMS Microbiology Letters, 177(1), 47-55.

Sae, L. N. (2007). The production of fungal mannanase, cellulase and xylanase using palm kernel cake as a substrate. Walailak J Sci & Tech. 4(1), 67-82.

Said, G. (1987). Biondustri: Penerapan Teknologi Fermentasi. Bogor. Pusat Antar Universitas Institut Pertanian Bogor

Van Zyl, W. H., Rose, S. H., Trollope, K., & Grgens, J. F. (2010). Fungal ?-mannanases: mannan hydrolysis, heterologous production and biotechnological applications. Process Biochemistry, 45(8), 1203-1213.

Vendruscolo, C. W., Ferrero, C., Pineda, E. A., Silveira, J. L., Freitas, R. A., Jimnez-Castellanos, M. R., & Bresolin, T. M. (2009). Physicochemical and mechanical characterization of galactomannan from Mimosa scabrella: Effect of drying method. Carbohydrate Polymers, 76(1), 86-93.

Wahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concentrations of Mus Musculus serum Exposed by Mycobacterium tuberculosis. Biosaintifika: journal of Biology & Biology Education, 8(1), 12-17.

Yopi, Susilaningsih, D., Thontowi, A., Purnawan, A., Djohan, A. C., Fahrurrozi, & Lisdiyanti, P. (2012). Study on Hemicellulolytic Bacteria: Production of Oligosaccharides from Palm Kernel Cake using Fermentation. Proceedings in Internasional Seminar Advances in Biological Science:Contribution Towards a Better Human Prosperity 111-113.

Yopi, Purnawan, A., Thontowi, A., Hermansyah, H., & Wijanarko, A. (2006). Preparasi mannan dan mannanase kasar dari bungkil kelapa sawit. J. Teknol, 4(20), 312-319.

Yopi, Thontowi, A., Susilaningsih, D., & Lisdiyanti, P. (2006) Analisis mikroba mannolitik dari strain BTCC. Prosiding Seminar Nasional Bioteknologi 442-448.

Yopi, Susilaningsih, D., Purnawan, A., Hermansyah, Thontowi, A., Djohan, A. C. (2010). Praharyawan S,LisdiyantiPalm kernel cake biomass fermentation using stirrer fermentor for mannanase and saccharides production. Prosiding Asean-Korea Symposium and Workshop on Biorefinery Technology 455-459.




DOI: https://doi.org/10.15294/biosaintifika.v9i1.9201

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.