Preparation of Polyelectrolyte Complex Films of Chitosan-Alginate Incorporated by Eugenol and its Potency as an Antioxidant Packaging

Baiq Amelia Riyandari


Preparation of PEC chitosan-alginate films incorporated by eugenol has been investigated. Incorporation of eugenol in chitosan-alginate films was conducted by using the different concentration of eugenol including 0.25% 0.5%, and 1% (% w/v). The effect of eugenol incorporation in chitosan-alginate films was investigated through some properties of the films such as tensile strength, elongation at break, transparency value, and water vapor permeability. Meanwhile, the effectiveness of eugenol incorporation as an active compound of the films was investigated from antioxidant activity of chitosan-alginate films incorporated eugenol. Polyelectrolyte complex (PEC) films of chitosan-alginate was occurred through molecular interaction between polycationic groups of chitosan and polyanionic groups of alginate. The formation of chitosan-alginate PEC films was synthesized at pH ± 4.0. Based on FTIR analysis, the ionic interaction between amine groups (–NH3+) and carboxylate groups (–COO¬) formed strongly. Characterization of films also indicated that PEC films of chitosan-alginate incorporated of eugenol was formed. Study showed that PEC chitosan-alginate films had good mechanical properties. Antioxidant activity assay through  fixed reaction time method  using DPPH radical (α,α-difenil-β- pikrilhidrazil) resulted in good percentage of radical scavenging activity (%RSA) from the films. The E3 films which contain 1% eugenol has 55.99% of  RSA value in 96 hours.


alginate; antioxidant; chitosan; eugenol; packaging

Full Text:



Bourtoom T., 2008, Plasticizer effect on the properties of biodegradable blend film from rice starch-chitosan. J. Sci. Technol. 30(1): 149–165.

Gata, M. O., Oshi, M. H., Rano, S. U., & Ndo, T. E. (2000). Antioxidant Activity of Eugenol and Related Monomeric and Dimeric Compounds. Chem. Pharm. Bull, 48: 1467–1469

Hosseini, M. ., Razavi, S. ., & Mousavi, M. . (2009). Antimicrobial, physical, and mechanical properties of chitosan-based films incorporated with thyme, clove, and cinnamon essential oils. Journal of Food Processing and Preservation, 33:727–743.

Kulig, D., & Zimoch-korzycka, A. (2017). Polyelectrolyte Complex on the Storage Quality of Pork. Molecules, 22(98): 1–16.

Lawrie, G., Keen, I., Drew, B., Chandler-temple, A., Rintoul, L., Fredericks, P., & Grøndahl, L. (2007). Interactions between Alginate and Chitosan Biopolymers Characterized Using FTIR and XPS. Biomacromolecules, 8:2533–2541.

Li, J., Miao, J., Wu, J., Chen, S., & Zhang, Q. (2014). Preparation and characterization of active gelatin-based fi lms incorporated with natural antioxidants. Food Hydrocolloids, 37:166–173.

Muratore, F., Goni, M.L., Strumia, M.C., Barbosa S.E., Ganan, N.A., & Martini R.E. (2019). Eugenol as an active component in food packaging materials. Eugenol: Biosynthesis, Toxicity, and Uses. 1-19.

Peng, Y., Wu, Y., & Li, Y. (2013). Development of tea extracts and chitosan composite films for active packaging materials. International Journal of Biological Macromolecules, 59:282–289.

Ramos, M., Jiménez, A., Peltzer, M., & Garrigós, M. C. (2012). Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. Journal of Food Engineering, 109(3):513–519.

Riyandari, B. A., Suherman, & Siswanta, D. (2018). The Physico-Mechanical Properties and Release Kinetics of Eugenol in Chitosan-Alginate Polyelectrolyte Complex Films as Active Food Packaging. Indonesian Journal of Chemistry, 18(1):82–91.

Rubilar, J. F., Cruz, R. M. S., Silva, H. D., Vicente, A. A., Khmelinskii, I., & Vieira, M. C. (2013). Physico-mechanical properties of chitosan films with carvacrol and grape seed extract. Journal of Food Engineering, 115(4):466–474.

Tongnuanchan, P., Benjakul, S., & Prodpran, T. (2013). Physico-chemical properties , morphology and antioxidant activity of film from fish skin gelatin incorporated with root essential oils. Journal of Food Engineering, 117(3):350–360.

Woranuch, S., & Yoksan, R. (2013). Eugenol-loaded chitosan nanoparticles : II . Application in bio-based plastics for active packaging. Carbohydrate Polymers, 96(2):586–592.

Yan, X., Khor, E., & Lim, L. (2000). PEC Films Prepared from Chitosan-Alginate Coacervates. Chem. Pharm. Bull, 48(7):941–946.


  • There are currently no refbacks.