The Effect of Solvent Ratio and Extraction Time on Antioxidant Activity and Flavonoid Concentration of Kedawung Leaf (Parkia Biglobosa) Through Microwave-Assisted Extraction

Ferika Indrasari, Buanasari Buanasari

Abstract

Kedawung (Parkia biglobosa) contains various ingredients such as alkaloids, saponins, tannins, flavonoids, and terpenoids. The flavonoid content in Kedawung is thought to have an antioxidant effect. Antioxidants are able to counteract free radicals that enter the body by donating electrons and binding them. Currently, the microwave-assisted extraction (MAE) method is widely used because the solute mass transfer from the sample matrix into the solvent is higher than the Soxhlet method. The following study aimed to know the effect of solvent ratio and extraction time on the extraction yield, flavonoid concentration, and antioxidant activity of kedawung leaf through microwave-assisted extraction. In this method, we used 40% ethanol to make the varied solute: solvent ratio such as 1:20, 1:30, 1:40, and 1:50. The extraction time used in this method was 4-7 minutes respectively. Microwave-assisted extraction has good performance to extract the active substance in Kedawung leaves. The highest yield 16.36%, total flavonoid content (57.32±2,2 mg QE/g extract), and DPPH scavenging activity (88.87±1.062%) was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction time of 6 minutes. This method promises to take the active substance in a short time.

Keywords

antioxidant; DPPH; kedawung; Microwave-Assisted Extraction (MAE)

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References

Buanasari, B., Sugiyo, W., Rustaman, H. 2021. Effect of Ultrasonic Assisted on The Degree of Deacetylation of Chitosan Extracted from Portunus Pelagicus. 10(1):17-23.

Chen, K. H, Miyazaki, T., Tsai, H. F., Bennett, J. E. 2007. The bZip transcription factor Cgap1p is involved in multidrug resistance and required for activation of multidrug transporter gene CgFLR1 in Candida glabrata. Gene. 386(1-2):63-72.

Kamaludin, H., Lihan, T., Ali Rahman, Z., Mustapha, M. A., Idris, W. M. R., Rahim, S. A. 2014. Integration of remote sensing, RUSLE and GIS to model potential soil loss and sediment yield (SY). Hydrology and Earth System Sciences Discussions. 10: 4567–4596.

Kataoka, H. 2019. Microwave Assisted Extraction (MAE). Science Direct: Encyclopedia of Analytical Science (Third Edition). 231-255

Komolafe, K., Akinmoladun, A., Olaleye, M. 2016. Methanolic Leaf Extract of Parkia biglobosa Protect against Doxorubicin-in ducet Cardiotoxicity in Rats. International Journal of Applied Research in Natural Products. 6(3): 39-47.

Kurutas, E. B. 2016. The Importance of Antioxidants Which Play the Role in cellular Response Against Oxidative/Nitrosative Stress: Current State. Nutrition Journal. 15(71):1-22.

Kusnadi, K., Dedi, D., Yunianta, Y., Arumingtyas, E. L. 2017. Extraction of Phenol Compounds and Antioxidant Activity from Cayenne Pepper Fruit by Microwave Assisted Extraction. Jurnal Teknologi Pertanian. 18(3): 181-190.

Halliwell, B. 2012. How to characterize an antioxidant- An update. Biochemical Social Symposia. 61:73–101

Hidayati J. R., Yudiati, E., Pringgenies, D., Arifin, A., Oktaviyanti, D. T. 2019. Antioxidant Activities, Total Phenolic Compound And Pigment Contents of Tropical Sargassum sp. Extract, Macerated In Different Solvents Polarity. Jurnal Kelautan Tropis. 22(1):73-80

Mandal, V., Mohan, Y., Hemalatha, S. 2007. Microwave-Assisted Extraction-An Innovative and Promoting Extraction Tool for Medical Plant Research. Pharmacognosy Reviews. 1 (1): 7-18.

Miollogo-Kone, H., Lompo, M., Kini, F., Asimi, S., Guissou, I. P., Nacoulma, O. 2009. Evaluation of Flavonoids and Total Phenolic Content of Stem Bark and Leaves of Parkia bigobosa (Jacq) Bent (Mimosaceae)-Free Radical Scavenging and Antimicrobial Activities. Research Journal of Medical Sciences. 3(2):70-74.

Panche, A. N., Diwan, A. D., Chandra, S. R. 2016. Flavonoids: an overview. Journal of Nutritional Science. 5: 47.

Sholikhah, E. N. 2016. Indonesian Medicinal Plants as Sources of Secondary Metabolites for Pharmaceutical Industry. Journal of Medical Science. 48(4):226-239.

Shuncheng, R., Yani, C., Suyun, H., Yiwei, C. 2013. Study on the Simultaneous Extraction of Total Flavonoid and Total Sponins from Corn Silks by Microwave-Assisted Technology. Medicinal Plant. 4(8):68-73.

Sulasmi, E. S., Indriwati, S. E., Suarsini, E. 2016. Preparation of Various Type of Medicinal Plants Simplicia as Material of Jamu Herbal. International Conference on Education in the 21th.21:1014-1024

Wang, Y., O’Leary, L.A., Rickard, R.S., Mason, C.A. 2010.. Geocoding capacity of birth defects surveillance programs: Results from the National Birth Defects Prevention Network Geocoding Survey. Journal of Registry Management. 37: 22-26.

Zhang, Q. W., Lin, L. G., Ye, W. C. 2018. Techniques for Extraction and Isolation of Natural Product: a Comprehensive review. Chinese Medicine. 13:1-26.

DOI: https://doi.org/10.15294/jbat.v11i1.33426

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