HIDROLISIS MINYAK KELAPA DENGAN LIPASE TERIMOBILISASI ZEOLIT PADA PEMBUATAN PERISA ALAMI

dwina moentamaria, Girlian Agaian, Meilita Mustika Ridhawati, Achmad Chumaidi, Nanik Hendrawati

Abstract

Free Fatty acid resulting from hydrolisis of various types of oil enzymatically has been great interest recently to save energy, in other hand that the product are environmentally friendly. Lipases as biocatalysts for synthesis reactions will be dissolved with the product, making difficult their reuse. Efficiency can be done with the use of enzyme immobilization, which can be used for repeated reaction. The products of free fatty acids from coconut oil by hydrolysis of lipase can be used as a natural substrate for making flavor that can be consumed and safe for health. The effect of free lipase and immobilization of lipase on hydrolisis were studied. Reaction time of hydrolisis was varied as 30, 60, 90, 120 and 150 minutes. The variation of concentration of lipase addition was 4, 5, 6, 7 and 8 % . The types of treatment were used in this research free lipase and the immobilized lipase. The results shows that the highest conversion on hydrolisis of coconut oil by using free lipase treatment was performed by 6 % of lipase addition with reaction time 60 minutes that are 52,31%. While, the highest conversion on hydrolisis of coconut oil by using the immobilized lipase was shown by 8% of lipase addition with reaction time 120 minutes that is 56,01%. The results of the hydrolysis process in the form of fatty acid was used as the base material esterification process resulting ester product (natural flavor). Ester yield was produced by free lipase esterification was 28,21 and 32,14 % in immobilized lipase esterification.

Keywords

Hydrolysis, Lipase, Immobilization, Zeolit, free lipase

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References

Banu, Oztur. (2001), Immobilization of Lipase from Candida rugosa on Hydropobic and Hydrophilic Support Turkey : Izmir Institute of Technology

Clber Calgarotoa, Robison P. Scherera, Selma Calgarotob, J. Vladimir Oliveiraa, Dbora de Oliveiraa, Sibele B.C. Pergher (2011) , Immobilization of porcine pancreatic lipase in zeolite MCM 22 with different Si/Al ratios, Applied Catalysis A: General 394 (2011) 101104

Fessenden & fessenden. 2006. Kimia Organik Jilid 1. Jakarta: Erlangga

H. Treichel, D. Oliveira, M.A. Mazutti, M. Di Luccio, V.J. Oliveira (2010), Effect of treatment with compressed propane on lipases hydrolytic activity, Food Bioprocess Technol. 3 (2010) 182188.

H.C.T. Cardias, C.C. Grininger, H.C. Trevisan, J.M. Guisan, R.L.C. Giordano (1999), Influence ofActivation on the Multipoint Immobilization of Penicillin G Acylase on Macroporous Silica, Braz. J. Chem. Eng., 16 (1999) 141-148

Handayani, Rini, Joko Sulistyo (2005), Transesterifikasi Ester Asam Lemak Melalui Pemanfaatan

Teknologi Lipase. B i o d i v e r s i t a s. Volume 6 , 164-167.

Lidya & Djenar (2000), Dasar Bioproses Direktorat Pembinaan dan Fenolinin dan Pengabdian Masyarakat Direktorat Jenderal Pendidikan Tinggi Departemen Pendidikan Nasional: Jakarta

Martin. A.James swarbrick and Arthur chammarata,(1993),Farmasi. Fisik Jakarta : Penerbit Universitas Indonesia

Macario, A. Katovic, G. Giordano, L. Forni, F. Carloni, A. Filippini, L. Setti ( 2005), Immobilization of Lipase on microporous and mesoporous materials studies of the support surfaces. Stud. Surf. Sci. Catal. 155, 381394

Pahoja, V.M., Dahot, M.U. dan Sethar, M.A (2001), Characteristic properties of lipase crude extract of Caesalpinia bounducella L. Seeds, Journal of Biological Sciences 1: 775-778.

Satyarthi J.K., Srinivas D., & Ratnasamy P (2011), Hydrolysis of vegetable oils and fats to fatty

acids over solid acid catalysts, Applied Catalysis A: General, 391:427435.

Setyopratomo, Puguh (2012), Produksi Asam Lemak Dari Minyak Kelapa Kelapa Dengan Proses Hidrolisis, Jurnal Teknik Kimia Vol.7, No.1 hal 26-31

Sri Wahyuni, Y (2005), Pengaruh Besar aukuran Partikel dan Suhu terhadap Solubilisasi Paracetamol Menggunakan Tween 80, Skripsi S1, Jurusan Farmasi,STIFI Perintis Padang.

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