Nita Rukminasari, Muhammad Lukman, Rahmadi Tambaru, Dwi Fajriyati Inaku, Suharto Suharto


Temperature and availability nutrients played an important role on growth and lipid production of microalgae. In this study, we examined the effect of increasing suhu and excessed and depleted NO3 on growth rate, biomass and free fatty acid concentration in the Spirulina sp and Skeletonema sp. Two microalgae were culture on Conway and Milne media for 21 days using continuous culture technique. There were four temperature treatments, such as 28oC, 30oC, 32oC and 34oC and three nutrient treatments, which were control nutrient treatment, without NO3 and two times NO3 concentrations from control treatments with three replicates for each treatments. Results found that increasing temperature significantly affected on biomass and concentration free fatty acid, meanwhile nutrient treatments affected on growth rate, biomass and concentration of organic Carbon. In general, increasing temperature was more affected on Spirulina sp in terms of increasing growth rate, biomass and free fatty acid concentration. However, Skeletonema sp was more responsive to availability of NO3 in the media culture for increasing free fatty acid, and percentage of free fatty acid per dry weight.


Increasing temperature; nutrient availability; free fatty acid; Spirulina sp.; Skeletonema sp; biodiesel

Full Text:



Bo ?lling, C., Fiehn, O. 2005. Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation. Plant Physiology. 139: 1995 2005.

Chen, M., Tang, H., Maa, H., Holland, T.C., Simon Ng, K.Y., Salley, S.O. 2011. Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresources Technology, 102: 1649 1655.

Chisti, Y. 2008. Biodiesel from microalgae beats bioethanol. Trends Biotechnology. 26:126131.

Hadi, P. U., Djulin, A., Zakaria, A. K., Darwis, V., Situmorang, J. 2006. Development prospect of energy alternative (biofuel): focus on jarak plant. Center of Socio-Economic Analysis and Agricultural Policy. Ministry of Agriculture. Jakarta.

Hanif, M., Dewanti, D. P. 2015. Design for microalgae conversion process to be a biofuel as eco-friendly innovation technology. Journal of Environmental Technology. 16(1): 18.

Hayun, A. 2009. Development priority of alternative energy (biofuel) in Indonesia. Prioritas pengembangan energi alternatif biofuel di Indonesia. Department of Industrial Engineering. Bina Nusantara University. Jakarta.

Ketaren, S. 1986. Introduction to oil and lipid technology. Indonesian University Press. Jakarta.

Li, X., Xu, H., Wu, Q. 2008. Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnology and Bioengineering. 98:764771.

Rukminasari, N. 2013. Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production. International Journal of Marine Science.3 (17): 135-144.

Rupprecht, J. 2009. From systems biology to fuelChlamydomonas reinhardtii as a model for a systems biology approach to improve biohydrogen production. Journal of Biotechnology. 142(1): 1020.

Sialve, B., Nicolas, B., Olivier, B. 2009. Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnology Advances.

Sobari, R., Susanto, A.B., Susilaningsih, D., Yunita, D. 2013. Kandungan lipid beberapa jenis sianobakteria laut sebagai bahan sumber penghasil biodiesel. Journal of Marine Science. 2(1): 112 119.

Song D, Fu J, Shi D. 2008. Exploitation of Oil-bearing Microalgae for Biodiesel. Chinese Journal of Biotechnology. 24(3):341-348.

Subejo. 2009. Energy crisis, biofuel and food. Faculty of Agriculture. Gajah Mada University. Yogyakarta.

Um, B. H., Kim, Y. S. 2009. Review: A chance for Korea to advance algal-biodiesel technology. Journal of Industrial and Engineering Chemistry. 15(1):1-7.

Vonshak, A., Cohen, Z., Richmond, A. 1985. The Feasibility of Mass Cultivation of Porphyridium. Biomass. 8: 13 25.

Walker, T. L., Purton, S., Becker, D. K., Collet, C. 2005. Microalgae as bioreactors. Plant Cell Reports. 24(11): 629-641.

Wang, B., Li, Y., Wu, N., Lan, C. Q. 2008. CO2 bio-mitigation using microalgae. Applied Microbiology and Biotechnology. 79:707-718.

Wijarnako, B., Putri, L.D. 2012. Lipid extraction from Ekstraksi lipid microalgae (Nannochloropsis sp.) with methanol and chloroform as a solvent. Journal of Industrial and Chemical Technology. 1(1): 130 138.


  • There are currently no refbacks.