Email this article Optimization of The Aqueous Enzymatic Extraction (AEE) of Rice Bran Oil With Cellulase Using Response Surface Methodology
Abstract
Rice Bran Oil (RBO) is an oil extracted from rice bran with unsaturated fat content according to World Health Organization (WHO) standards. The enzymatic extraction process of rice bran for oil extraction using cellulase enzymes is considered effective because it is capable of producing oil. Parameters of temperature and incubation time have an influence on the yield of oil produced. So that research is needed regarding the optimum conditions of the enzymatic extraction process including temperature and incubation time on the yield and levels of FFA RBO.Software Design Expert was used in this study to optimize RBO extraction with cellulase using the Response Surface Methodology (RSM) method in the Central Composite Design (CCD) factorial experimental design. The selected independent variables consisted of incubation temperatures, namely 35, 50, and 65 0C and incubation time for 2, 3, and 4 hours. The results show that the RBO yield is 1.7% and the minimum target for FFA levels is 8.4% at a temperature of 51.5 0C with an incubation time of 4 hours. Processing data with Design expert software produces an analysis of ANOVA experimental data. Incubation time has a significant level (p<0.05) on RBO yield and incubation temperature has a significant level (p<0.05) on RBO FFA.
Keywords
Full Text:
PDFReferences
American Oil Chemists’ Society (AOCS). 2009. AOCS Official Method Ca 5a-40: Free fatty acids. AOCS.
Arora, R., Toor, A. P., Wanchoo, R. K. 2015. Esterification of high free fatty acid rice bran oil: Parametric and kinetic study. Chemical and Biochemical Engineering Quarterly. 29(4): 617–623.
Bayuo, J., Pelig-Ba, K. B., Abukari, M. A. 2019. Optimization of adsorption parameters for effective removal of lead(II) from. Physical Chemistry: An Indian Journal. 14(1): 1–25.
Bhattacharya, S. 2021. Central composite design for response surface methodology and its application in pharmacy. In Response surface methodology in engineering science. IntechOpen.
Begum, A., Sarma, J., Borah, P., Moni Bhuyan, P., Saikia, R., Hussain Ahmed, T., Karki, S., Rupa Dowarah, J., Gupta, P., R Khanna, R., Rai, L., Lalhmangaihzuali, J., Goswami, A., Chowdhury, P. 2015. Microwave (MW) Energy in Enzyme Deactivation: Stabilization of Rice Bran from Few Widely Consumed Indigenous Rice Cultivars (Oryza sativa L.) from Eastern Himalayan Range. Current Nutrition & Food Science. 11(3): 240–245.
Candioti, L. V., De Zan, M. M., Cámara, M. S., Goicoechea, H. C. 2014. Experimental design and multiple response optimization. Using the desirability function in analytical methods development. Talanta. 124: 123–138.
Casas, M. P., González, H. D. 2017. Enzyme-assisted aqueous extraction processes. In Water Extraction of Bioactive Compounds. In Water Extraction of Bioactive Compounds From Plants to Drug Development. Elsevier.
Chelladurai, S. J. S., Murugan, K., Ray, A. P., Upadhyaya, M., Narasimharaj, V., Gnanasekaran, S. 2020. Optimization of process parameters using response surface methodology: A review. Materials Today: Proceedings. 37(2): 1301–1304.
Damayanti, A., Triwibowo, B., Megawati, M., Azhari, M., Fadriana, S. A. 2021. Optimization of Anthocyanin Extraction from Cockspur Coral (Erythrina Crista-Galli L.) Petals with Microwave-Assisted Extraction (MAE) using Response Surface Methodology. ASEAN Journal of Chemical Engineering. 21(2): 143.
Di Pietro, M. E., Mannu, A., Mele, A. 2020. NMR determination of free fatty acids in vegetable oils. Processes. 8(4): 410.
Fraterrigo, G., S., Tommasi, T., Fino, D. 2021. A short review of green extraction technologies for rice bran oil. Biomass Conversion and Biorefinery. 11(2): 569–587.
Garba, U., Singanusong, R., Jiamyangyuen, S., Thongsook, Ti. 2019. (PDF) Extraction and utilization of rice bran oil: A review extraction and utilization of rice bran oil: A review. Italian Journal of Fatty Substances. 96(3): 161–170.
Giovannoni, M., Gramegna, G., Benedetti, M., Mattei, B. 2020. Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility. Frontiers in Bioengineering and Biotechnology. 8.
Gokhale, A. A., Lu, J., Lee, I. 2013. Immobilization of cellulase on magnetoresponsive graphene nano-supports. Journal of Molecular Catalysis. B, Enzymatic, Complete. 90: 76–86.
Karthika, M. S. 2020. Effect of Enzyme Assisted Aqueous Extraction and Ohmic Heating Treatment on Rice Bran Oil Recovery. Indian Journal of Pure & Applied Biosciences. 8(5): 177–184.
Khelifa, H., Bezazi, A., Boumediri, H., Garcia del Pino, G., Reis, P. N. B., Scarpa, F., & Dufresne, A. 2021. Mechanical characterization of mortar reinforced by date palm mesh fibers: Experimental and statistical analysis. Construction and Building Materials. 300: 124067.
Kuddus, M. 2018. Introduction to food enzymes. Enzymes in Food Biotechnology: Production, Applications, and Future Prospects. 1–18.
Li, P., Zhang, W., Han, X., Liu, J., Liu, Y., Gasmalla, M. A. A., Yang, R. 2017. Demulsification of oil-rich emulsion and characterization of protein hydrolysates from peanut cream emulsion of aqueous extraction processing. Journal of Food Engineering. 204: 64–72.
Loypimai, P., Moongngarm, A., Chottanom, P. 2015. Impact of stabilization and extraction methods on chemical quality and bioactive compounds of rice bran oil. Emirates Journal of Food and Agriculture. 27(11): 849–856.
Mahesar, S. A., Sherazi, S. T. H., Khaskheli, A. R., Kandhro, A. A., Uddin, S. 2014. Analytical approaches for the assessment of free fatty acids in oils and fats. Analytical Methods. 6(14): 4956–4963.
Maran, J. P., Manikandan, S., Thirugnanasambandham, K., Vigna Nivetha, C., Dinesh, R. 2013. Box–Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydrate Polymers. 92(1): 604–611.
Mat Yusoff, M., Gordon, M. H., Niranjan, K. 2015. Aqueous enzyme assisted oil extraction from oilseeds and emulsion de-emulsifying methods: A review. Trends in Food Science & Technology. 41(1): 60–82.
Moreno, J. J. M., Palmer, P. A., Sesé, A. A., Cajal, B. B. 2013. Using the R-MAPE index as a resistant measure of forecast accuracy. Psicothema. 25(4): 500–506.
Mounika, E., Smith, Dr. D., Edukondalu, Dr. L., Raja, D. S. 2020. Quality parameters of ultrasound assisted aqueous enzymatic extraction of rice bran oil. International Journal of Chemical Studies. 8(5): 1765–1768.
Mwaurah, P. W., Kumar, S., Kumar, N., Attkan, A. K., Panghal, A., Singh, V. K., Garg, M. K. 2020. Novel oil extraction technologies: Process conditions, quality parameters, and optimization. Comprehensive Reviews in Food Science and Food Safety. 19(1): 3–20.
Noureen, H., Alam, S., Al Ayoubi, S., Qayyum, A., Sadiqi, S., Atiq, S., Naz, A., Bibi, Y., Ahmed, W., Khan, M. M., Sammi, S., Liaquat, M., Ahmad, S. 2021. Mechanism of rice bran lipase inhibition through fermentation activity of probiotic bacteria. Saudi Journal of Biological Sciences. 28(10): 5841–5848.
Pandey, R., Shrivastava, S. L. 2018. Comparative evaluation of rice bran oil obtained with two-step microwave assisted extraction and conventional solvent extraction. Journal of Food Engineering. 218: 106–114.
Rao, K. V., Murthy, P. B. G. S. N. 2018. Modeling and optimization of tool vibration and surface roughness in boring of steel using RSM, ANN and SVM. Journal of Intelligent Manufacturing. 29(7): 1533–1543.
Qian, J., Zhao, X., Zhao, C., Yang, H., Gou, L., Wang, W., Guo, H. 2021. Pretreatment Camellia Seeds by Protease and Application to Extraction of Camellia Oil. European Journal of Lipid Science and Technology. 123(1): 2000223.
Vallabha, V. S., Indira, T. N., Jyothi Lakshmi, A., Radha, C., Tiku, P. K. 2015. Enzymatic process of rice bran: a stabilized functional food with nutraceuticals and nutrients. Journal of Food Science and Technology. 52(12): 8252–8259.
Salimon, J., Abdullah, B. M., Salih, N. 2011. Hydrolysis optimization and characterization study of preparing fatty acids from Jatropha curcas seed oil. Chemistry Central Journal. 5(1): 1–9.
Sharif, M. K., Butt, M. S., Anjum, F. M., Khan, S. H. 2014. Rice Bran: A Novel Functional Ingredient. Critical Reviews in Food Science and Nutrition. 54(6): 807–816.
Singh, S., Singla, P., Arora, S. 2015. Statistical, Diagnostic And Response Surface Analysis of Nefopam Hydrochloride Nanospheres Using 35 Box-Behnken Design. International Journal of Pharmacy and Pharmaceutical Sciences. 7(10): 89–101.
Souza, T. S. P., Dias, F. F. G., Koblitz, M. G. B., Bell, M. L. N. de M.. 2019. Aqueous and Enzymatic Extraction of Oil and Protein from Almond Cake: A Comparative Study. Processes, 7(7): 472.
Tambunan, A. H., Situmorang, J. P., Silip, J. J., Joelianingsih, A., Araki, T. 2012. Yield and physicochemical properties of mechanically extracted crude Jatropha curcas L oil. Biomass and Bioenergy. 43: 12–17.
Xu, D., Hao, J., Wang, Z., Yang, H., Gao, Q., Ma, N., Wang, J., Ma, Y., Zhang, M.. 2020. Aqueous enzymatic extraction of oil from rice bran and its quality evaluation. Authorea Preprints.
Zaidel, D. N. A., Muhamad, I. I., Daud, N. S. M., Muttalib, N. A. A., Khairuddin, N., Lazim, N. A. M. 2019. Production of biodiesel from rice bran oil. Biomass, Biopolymer-Based Materials, and Bioenergy. pp. 409–447. Elsevier.
Zigoneanu, I. G., Williams, L., Xu, Z., Sabliov, C. M. 2008. Determination of antioxidant components in rice bran oil extracted by microwave-assisted method. Bioresource Technology. 99(11): 4910–4918.
Refbacks
- There are currently no refbacks.
