Enzymatic Modification of Cellulose Based Materials for Pomising of Bioethanol Production

  • Asmaul Karima Institut Teknologi Sepuluh Nopember, Indonesia
  • Sri Nurhatika Institut Teknologi Sepuluh Nopember, Indonesia
  • Endry Nugroho Prasetyo Institut Teknologi Sepuluh Nopember, Indonesia

Abstract

Cellulose is the most abundant D-glukosabiopolymer in nature with b-1,4 glycosidic bonds whichhas crystalline structure and difficult to hydrolized. Saccharification or enzymatic hydrolysis of cellulosic materials could be producedglucose.In this study, cellulase hasbeen produced from Trichoderma harzianum and Trichoderma sp.LM 1039. The result of enzyme purification showedthat highest activity on Trichoderma harzianumis amount of  233,89 U/ml and the protein content is amount of 0,174 mg/ml while on Trichoderma sp.LM 1039 of 223,334 U/ml and 0,159 mg/ml. Isoelectric point and SDS-PAGE analysis showed that the isoelectricpoint of enzyme ison pH 5 by the molecules weightis on 40,36 kDa. The result of enzymatic hydrolysis using crude cellulase on the seaweed substrate,  newspapers and Whatman no.1 filter paper showed that the highestto the lowest reducing sugar on seaweed substrate is amount of 1,43-1,44 mM/ml, 0,54-0,70 mM/ml on newspaper, and 0,55-0,78 mM/ml on Whatman filter paper.The FTIRanalysis on newspaper showed that on 894 cm-1 changes to the eter groups (C-O-C) in cellulose chain.

Published
2017-03-08